Volume 31, Issue 1 (April 2020)
Studies in Medical Sciences 2020, 31(1): 7-14 |
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Vardast M R, Ranjkeshzadeh N. NEW METHOD FOR DETERMINATION OF AMIODARONE IN SERUM WITH DISPERSIVE LIQUID-LIQUID MICROEXTRACTION BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH EXPERIMENTAL METHOD. Studies in Medical Sciences 2020; 31 (1) :7-14
URL: http://umj.umsu.ac.ir/article-1-4596-en.html
URL: http://umj.umsu.ac.ir/article-1-4596-en.html
Department of Medicinal Chemistry, School of Pharmacy, Urmia University of Medical Sciences, Urmia, Iran (Corresponding Author) , mrvardast@gmail.com
Abstract: (3024 Views)
Background & Aims: Amiodarone is an anti-arthritis drug (III) used to treat various types of arrhythmias. Determining the concentration of this drug in the blood of patients is important for the administration of this drug and its interaction with other drugs (due to its high interaction with other drugs). It is necessary to use precision instruments such as HPLC with expensive detectors, but the analysis is often time-consuming and the methods of extraction have a high cost. Therefore, the liquid-liquid extraction method for rapid and accurate measurement of this medication was used in serum.
Materials & Methods: The liquid-liquid extraction method was used with a variety of solvent extraction and different types of solvent. After selecting suitable solvents and optimizing various experimental parameters, this method was used to extract and measure the concentration of amiodarone in Blood serum.
Results: The results showed that carbon tetrachloride is suitable as a solvent extraction and acetonitrile is suitable as a solvent, and their optimal volume is 75 and 200 μl, respectively, and the optimal volume of the serum is 100 μl and the appropriate pH is in the two extraction steps 1 and 6. Mean calibration curve of Amiodarone is linear in the range of 0.05-0.0 μg / ml and has a relative standard deviation of 3.79%.
Conclusion: The results indicate that this method is suitable for measuring amiodarone in blood serum and reduces the time and cost of the analysis to a satisfactory level and has a good repeatability. With this method, it is easy to measure the effect of serum tissue in measuring amiodarone.
Materials & Methods: The liquid-liquid extraction method was used with a variety of solvent extraction and different types of solvent. After selecting suitable solvents and optimizing various experimental parameters, this method was used to extract and measure the concentration of amiodarone in Blood serum.
Results: The results showed that carbon tetrachloride is suitable as a solvent extraction and acetonitrile is suitable as a solvent, and their optimal volume is 75 and 200 μl, respectively, and the optimal volume of the serum is 100 μl and the appropriate pH is in the two extraction steps 1 and 6. Mean calibration curve of Amiodarone is linear in the range of 0.05-0.0 μg / ml and has a relative standard deviation of 3.79%.
Conclusion: The results indicate that this method is suitable for measuring amiodarone in blood serum and reduces the time and cost of the analysis to a satisfactory level and has a good repeatability. With this method, it is easy to measure the effect of serum tissue in measuring amiodarone.
Keywords: Amiodarone, Dispersive liquid-liquid microextraction, High-performance liquid chromatography
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