Volume 36, Issue 3 (9-2025)
Studies in Medical Sciences 2025, 36(3): 195-202 |
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Hajati A, Mahdavi Fikjvar E, Mohammadi Jonaghani M, Satvat P, Amini N. Chemical Profile, Antioxidant Potential, and Antibacterial Activity of Heracleum persicum Essential Oil against Staphylococcus aureus and Escherichia coli. Studies in Medical Sciences 2025; 36 (3) :195-202
URL: http://umj.umsu.ac.ir/article-1-6444-en.html
URL: http://umj.umsu.ac.ir/article-1-6444-en.html
Amirmohammad Hajati 
, Edris Mahdavi Fikjvar * 
, Maedeh Mohammadi Jonaghani , Parisa Satvat , Nooshin Amini
, Edris Mahdavi Fikjvar * , Maedeh Mohammadi Jonaghani , Parisa Satvat , Nooshin Amini
Medical Biotechnology Research Centre, School of Paramedicine, Guilan University of Medical Sciences, Guilan, Iran , ias.intl.scientists@gmail.com
Abstract: (54 Views)
Abstract
Background and Aim: The medicinal plant Heracleum persicum has attracted attention due to its bioactive compounds and antimicrobial properties. This study aimed to identify the constituents of essential oil from dried H. persicum fruits and evaluate its antibacterial and antioxidant activities.
Materials and Methods: Essential oil was extracted from dried fruits using a Clevenger-type distillation apparatus. The components were identified by gas chromatography–mass spectrometry. Total phenolic and flavonoid contents were determined. Antibacterial activity against Staphylococcus aureus and Escherichia coli was assessed using the disk diffusion method and by determining the minimum inhibitory concentration. Antioxidant activity was measured using the DPPH radical scavenging assay.
Results: Thirty-one compounds were identified, with n-hexyl butyrate (49.98%) and octyl acetate (15.39%) being the most abundant. The essential oil contained high levels of phenolics (108.25 ± 0.1 mg/g) and flavonoids (21.29 ± 0.1 mg/g). Its antioxidant activity was lower than BHT (IC50 = 1443.92 µg/mL). Significant antibacterial effects were observed against Staphylococcus aureus (MIC = 0.2 mg/mL) and, to a lesser extent, Escherichia coli (MIC = 0.5 mg/mL), whereas activity against other tested bacteria was weaker (MIC > 16 mg/mL).
Conclusion: The essential oil of H. persicum fruits contains bioactive compounds and exhibits notable antibacterial activity, particularly against Staphylococcus aureus. Its antioxidant activity was lower than the synthetic antioxidant BHT, and further studies are required to assess safety and efficacy in in vivo conditions.
Background and Aim: The medicinal plant Heracleum persicum has attracted attention due to its bioactive compounds and antimicrobial properties. This study aimed to identify the constituents of essential oil from dried H. persicum fruits and evaluate its antibacterial and antioxidant activities.
Materials and Methods: Essential oil was extracted from dried fruits using a Clevenger-type distillation apparatus. The components were identified by gas chromatography–mass spectrometry. Total phenolic and flavonoid contents were determined. Antibacterial activity against Staphylococcus aureus and Escherichia coli was assessed using the disk diffusion method and by determining the minimum inhibitory concentration. Antioxidant activity was measured using the DPPH radical scavenging assay.
Results: Thirty-one compounds were identified, with n-hexyl butyrate (49.98%) and octyl acetate (15.39%) being the most abundant. The essential oil contained high levels of phenolics (108.25 ± 0.1 mg/g) and flavonoids (21.29 ± 0.1 mg/g). Its antioxidant activity was lower than BHT (IC50 = 1443.92 µg/mL). Significant antibacterial effects were observed against Staphylococcus aureus (MIC = 0.2 mg/mL) and, to a lesser extent, Escherichia coli (MIC = 0.5 mg/mL), whereas activity against other tested bacteria was weaker (MIC > 16 mg/mL).
Conclusion: The essential oil of H. persicum fruits contains bioactive compounds and exhibits notable antibacterial activity, particularly against Staphylococcus aureus. Its antioxidant activity was lower than the synthetic antioxidant BHT, and further studies are required to assess safety and efficacy in in vivo conditions.
Keywords: Antibacterial agent, Escherichia coli, Essential oil, Heracleum persicum, Staphylococcus aureus
Type of Study: Research |
Subject:
فارماکولوژی
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