Volume 36, Issue 3 (9-2025)
Studies in Medical Sciences 2025, 36(3): 186-194 |
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Torabi P, Rostami Motamed H, Ebadi M. Investigation of the Effects of 5-Fluorouracil and Green Synthesized Silver Chloride Nanoparticles on Embryonic Kidney Cells (HEK293). Studies in Medical Sciences 2025; 36 (3) :186-194
URL: http://umj.umsu.ac.ir/article-1-6443-en.html
URL: http://umj.umsu.ac.ir/article-1-6443-en.html
Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran , aic.intl.conferences@gmail.com
Abstract: (57 Views)
Abstract
Background & Aim:
Despite its anticancer efficacy, 5-Fluorouracil exhibits toxicity toward normal cells. Similarly, silver chloride nanoparticles (AgCl NPs), although known for their anticancer potential, raise safety concerns. Due to limited data on the combined effects of these two agents on normal renal cells, this study aimed to evaluate the individual and combined cytotoxic effects of 5-Fluorouracil and AgCl NPs on HEK293 cells.
Materials & Methods:
HEK293 cells were treated with various concentrations of 5-Fluorouracil, AgCl NPs, and their combination. Cell viability was assessed using the MTT assay, and IC₅₀ values were calculated. Statistical analysis was performed using one-way ANOVA and Tukey’s post hoc test.
Results:
All treatments reduced cell viability in a dose-dependent manner. IC₅₀ values were calculated as 125 µg/ml for AgCl NPs, 174 µg/ml for 5FU, and 181 µg/ml for the combination. The combined treatment exhibited a higher IC₅₀ than either single agent, suggesting potential protective interactions.
Conclusion:
Co-treatment with 5-Fluorouracil and AgCl NPs may reduce cytotoxicity to normal cells while maintaining therapeutic efficacy. These findings highlight the need for further in vivo studies and mechanistic investigations to optimize safe and effective nanoformulation-based therapies.
Background & Aim:
Despite its anticancer efficacy, 5-Fluorouracil exhibits toxicity toward normal cells. Similarly, silver chloride nanoparticles (AgCl NPs), although known for their anticancer potential, raise safety concerns. Due to limited data on the combined effects of these two agents on normal renal cells, this study aimed to evaluate the individual and combined cytotoxic effects of 5-Fluorouracil and AgCl NPs on HEK293 cells.
Materials & Methods:
HEK293 cells were treated with various concentrations of 5-Fluorouracil, AgCl NPs, and their combination. Cell viability was assessed using the MTT assay, and IC₅₀ values were calculated. Statistical analysis was performed using one-way ANOVA and Tukey’s post hoc test.
Results:
All treatments reduced cell viability in a dose-dependent manner. IC₅₀ values were calculated as 125 µg/ml for AgCl NPs, 174 µg/ml for 5FU, and 181 µg/ml for the combination. The combined treatment exhibited a higher IC₅₀ than either single agent, suggesting potential protective interactions.
Conclusion:
Co-treatment with 5-Fluorouracil and AgCl NPs may reduce cytotoxicity to normal cells while maintaining therapeutic efficacy. These findings highlight the need for further in vivo studies and mechanistic investigations to optimize safe and effective nanoformulation-based therapies.
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