Volume 36, Issue 2 (6-2025)
Studies in Medical Sciences 2025, 36(2): 123-130 |
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Bafahm D, Ghane M, Mohammadi M. Extraction of probiotic bacteria from camel milk and its effect on liver cancer. Studies in Medical Sciences 2025; 36 (2) :123-130
URL: http://umj.umsu.ac.ir/article-1-6428-en.html
URL: http://umj.umsu.ac.ir/article-1-6428-en.html
Department of Biology, IsI.C., Islamic Azad University, Islamshahr, Iran , mh_mohamadi@yahoo.com
Abstract: (218 Views)
Background Nowadays, the use of natural and alternative therapies for the prevention and treatment of cancer, including probiotics such as lactic acid bacteria, has gained increasing attention. This study aimed to investigate the anticancer, pro-apoptotic, and antioxidant effects of lactic acid bacteria isolated from camel milk in Mashhad on liver cancer, as well as their impact on the expression of BAX and BCL-2 genes using the Real-time PCR method.
Methods Camel milk samples were collected from Mashhad, and the isolated strains were identified using biochemical tests. The anticancer, anti-apoptotic, and antioxidant effects of the isolated LAB were evaluated using laboratory tests, including the MTT assay and the DPPH radical scavenging method. The effect of isolated LAB on BAX and BCL-2 gene expression in HCC cells was investigated using real-time PCR.
Results Eight Lactobacillus strains with probiotic properties were identified. Two strains, A4 and A8, had the highest probiotic activity. The DPPH radical scavenging activity for A4 was between 2–23% and for A8 between 2–19%. Strains A4 and A8 showed time- and dose-dependent toxicity on HepG2 hepatocytes. The IC₅₀ values for the strain A4 were 30.66 μg/mL at 48 hours and 51.89 μg/mL at 72 hours, while for the strain A8, they were 32.03 μg/mL at 48 hours and 67.29 μg/mL at 72 hours, respectively. Caspase activity increased in hepatocytes after treatment with A4 and A8.
Conclusion The findings of this study demonstrated the anticancer, anti-apoptotic, and antioxidant effects of LAB isolated from camel milk in Mashhad on liver cancer cells and their influence on BAX and BCL-2 gene expression.
Methods Camel milk samples were collected from Mashhad, and the isolated strains were identified using biochemical tests. The anticancer, anti-apoptotic, and antioxidant effects of the isolated LAB were evaluated using laboratory tests, including the MTT assay and the DPPH radical scavenging method. The effect of isolated LAB on BAX and BCL-2 gene expression in HCC cells was investigated using real-time PCR.
Results Eight Lactobacillus strains with probiotic properties were identified. Two strains, A4 and A8, had the highest probiotic activity. The DPPH radical scavenging activity for A4 was between 2–23% and for A8 between 2–19%. Strains A4 and A8 showed time- and dose-dependent toxicity on HepG2 hepatocytes. The IC₅₀ values for the strain A4 were 30.66 μg/mL at 48 hours and 51.89 μg/mL at 72 hours, while for the strain A8, they were 32.03 μg/mL at 48 hours and 67.29 μg/mL at 72 hours, respectively. Caspase activity increased in hepatocytes after treatment with A4 and A8.
Conclusion The findings of this study demonstrated the anticancer, anti-apoptotic, and antioxidant effects of LAB isolated from camel milk in Mashhad on liver cancer cells and their influence on BAX and BCL-2 gene expression.
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