Volume 35, Issue 11 (12-2024)
Studies in Medical Sciences 2024, 35(11): 938-949 |
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Research code: 73992
Ethics code: IR.TBZMED.VCR.REC.1403.098
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Andishmand H, Ezzati Nazhad Dolatabadi J. DESIGNING AND FABRICATION OF SOLID LIPID NANOPARTICLES CONTAINING KETOTIFEN FUMARATE AND EVALUATION OF ITS STRUCTURAL AND BIOLOGICAL PROPERTIES. Studies in Medical Sciences 2024; 35 (11) :938-949
URL: http://umj.umsu.ac.ir/article-1-6383-en.html
URL: http://umj.umsu.ac.ir/article-1-6383-en.html
Associate professor, Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran (Corresponding Author) , ezzatij@tbzmed.ac.ir
Abstract: (485 Views)
Background & Aims: Solid lipid nanoparticles (SLNs) have been introduced as an alternative to other traditional colloidal carriers, and they can largely overcome the limitations associated with them. The aim of this research was to design and manufacture a new formulation of lipid nanoparticles loaded with ketotifen fumarate and evaluate its structural properties.
Materials & Methods: Solid lipid nanoparticles loaded with ketotifen fumarate (KT) were prepared using the hot-probe sonication homogenization method at 80 ℃ in a weight-weight ratio of 1:0.2:0.03:1 Comprital:ketotifen fumarate:Tween 80:Pluronic 407.
Results: Particle size, polydispersity index (PDI), encapsulation efficiency (EE), and loading capacity (LC) of SLNs loaded with KT in a ratio of 1:0.2:0.03:1 w/w Comprital:ketotifen fumarate:Tween 80:Pluronic 407 were obtained as 61.97±4.8 nm, 0.551±0.06, 62.82±2.97%, and 12.56±0.594%, respectively. The results showed that within 24 hours, the cumulative release of KT for free and loaded drug in SLNs was 94.88% and 73.01%, respectively. The survival of A549 cells treated with SLNs loaded with KT was better than that of the free drug after 24 and 48 hours, and SLNs loaded with KT did not show significant cytotoxicity.
Conclusion: The results of the Zetasizer were consistent with the results of the transmission electron microscopy (TEM) images, which showed the spherical shape of SLNs in nanoscale dimensions. Cytotoxicity studies confirmed the low toxicity of KT-loaded SLNs. Ease of fabricating SLNs, a high level of EE, and favorable biocompatibility were the advantages of SLNs loaded with KT.
Keywords: Ketotifen fumarate, Encapsulation, Nanocarriers, Lipid-based nanoparticles, Controlled release, Cytotoxicity
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