Volume 35, Issue 5 (August 2024)
Studies in Medical Sciences 2024, 35(5): 425-433 |
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Ethics code: IR.QUMS.REC.1403.073
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Safavi S, Peyman gohar M, Khakpour M, Fardsanei F, Nikkhahi F. EVALUATION OF THE EFFECT OF CHLORHEXIDINE AND EDTA ON MULTI-DRUG RESISTANT STRAINS OF KLEBSIELLA PNEUMONIAE AND BIOFILM-PRODUCING AND NON-PRODUCING ESCHERICHIA COLI. Studies in Medical Sciences 2024; 35 (5) :425-433
URL: http://umj.umsu.ac.ir/article-1-6272-en.html
URL: http://umj.umsu.ac.ir/article-1-6272-en.html
Assistant Professor of Medical Bacteriology, Medical Microbiology Research Center, Qazvin, Iran (Corresponding Author) , farhadnikkhahi@gmail.com
Abstract: (287 Views)
Background & Aims: Escherichia coli and Klebsiella pneumoniae are important bacteria that cause hospital-acquired infections and have become resistant to beta-lactam antibiotics through the acquisition of beta-lactamase-encoding plasmids. Resistance to disinfectants has also been observed in these strains. Therefore, the aim of this study was to investigate the antimicrobial effect of the disinfectant chlorhexidine on multidrug-resistant strains of Escherichia coli and Klebsiella pneumoniae.
Materials & Methods: Clinical samples were collected from the intensive care unit of Qazvin hospital. All isolates were analysed by phenotypic method. Finally, the combined effect of chlorhexidine and EDTA on the collected strains was performed.
Results: 45% of the 70 Escherichia coli isolates were MDR and 83.3% of the 74 Klebsiella pneumoniae isolates. All samples were equally sensitive to chlorhexidine. EDTA showed no synergistic effect with this substance.
Conclusion: The results of our study show that chlorhexidine can effectively destroy Escherichia coli and Klebsiella pneumoniae producing and non-producing biofilm strains. Therefore, this disinfectant can be used at low concentrations to control antibiotic resistance in multidrug-resistant Escherichia coli and Klebsiella pneumoniae bacteria in hospital-acquired infections.
Materials & Methods: Clinical samples were collected from the intensive care unit of Qazvin hospital. All isolates were analysed by phenotypic method. Finally, the combined effect of chlorhexidine and EDTA on the collected strains was performed.
Results: 45% of the 70 Escherichia coli isolates were MDR and 83.3% of the 74 Klebsiella pneumoniae isolates. All samples were equally sensitive to chlorhexidine. EDTA showed no synergistic effect with this substance.
Conclusion: The results of our study show that chlorhexidine can effectively destroy Escherichia coli and Klebsiella pneumoniae producing and non-producing biofilm strains. Therefore, this disinfectant can be used at low concentrations to control antibiotic resistance in multidrug-resistant Escherichia coli and Klebsiella pneumoniae bacteria in hospital-acquired infections.
Type of Study: Research |
Subject:
میکروبیولوژی
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