Volume 32, Issue 5 (August 2021)                   Stud Med Sci 2021, 32(5): 317-328 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Khang nikfarjam S, Baharara J, Nejad shahrokhabady K. CYTOTOXIC EFFECTS OF ARTEMISIA ABSINTHIUM EXTRACT ON A2780 CELL LINE (OVARIAN CANCER) AND ALTERATION OF APOPTOTIC GENES EXPRESSION LEVELS. Stud Med Sci. 2021; 32 (5) :317-328
URL: http://umj.umsu.ac.ir/article-1-5552-en.html
Islamic Azad University, Mashhad , baharara78@gmail.com
Abstract:   (423 Views)
Background & Aims: Ovarian cancer is the third most common cancer in women. Artemisia is one of the most commonly used medicinal plants, and Artemisia absinthium is one of the important species of this genus. The aim of this study was to evaluate the effect of cytotoxicity and the ability to induce apoptosis methanolic extract of A. absinthium in A2780 cell line (human ovarian cancer).
Materials & Methods: In this experimental study, methanolic extract of A. absinthium was prepared. MTT assay was used to evaluate the cytotoxic effects of A. absinthium extract on A2780 cancer cell proliferation and DAPI staining and flow cytometry analysis were used to evaluate the ability to induce apoptosis in treated cells with A. absinthium extract. Changes in "BAX, Caspase 3,9, p53" genes expression were evaluated by Real-time PCR. Quantitative data were analyzed by one-way ANOVA at a significant level of p<0.05.
Results: The MTT assay results showed that methanolic extract of A. absinthium, inhibits proliferation of ovarian cancer A2780 cells in a concentration-dependent manner (p<0.001***). Morphological observation with DAPI staining and flow cytometry analysis results showed an increased percentage of apoptotic cells. The real-time PCR results showed an increase in the expression of apoptotic genes "BAX, Caspase 3,9, p53" in treatment groups p <0.01**; p<0.001***.
Conclusion: The results of this study showed that the methanolic extract of A. absinthium inhibits the proliferation of A2780 cancer cells and induces apoptosis in these cells.
Full-Text [PDF 951 kb]   (192 Downloads)    
Type of Study: Research | Subject: ژنتیک

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.