Volume 31, Issue 12 (March 2021)                   Studies in Medical Sciences 2021, 31(12): 921-926 | Back to browse issues page

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Ebrahimi R, Yakhchali M, Malekinejad H. IN-VITRO ASSESSMENT OF RESISTANCE TO ALBENDAZOLE AND FENBENDAZOLE IN GASTROINTESTINAL NEMATODES. Studies in Medical Sciences 2021; 31 (12) :921-926
URL: http://umj.umsu.ac.ir/article-1-4921-en.html
Professor, Department of Pathobiology, Parasitology Division, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. (Corresponding Author) , m.yakhchali@urmia.ac.ir
Abstract:   (1459 Views)
Background & Aims: Drug resistance is a great concern worldwide and in Iran. This study was carried out to assess drug resistance of gastrointestinal nematodes to Albendazole (Alb) and Fenbendazole (Feb) in sheep.
Materials & Methods: A total number of 90 fresh fecal samples were directly collected from the rectum of infected sheep with an average egg per gram of feces (EPG)≤150. A dilution of 1000 eggs per 2mL was added to each well of control, Alb (0.1µg/ml) and Feb (0.1µg/ml) groups and incubated. To determine drug resistance, a lethal dose of 50% (LD50) was calculated based on percentage of the hatched eggs and first larvae stage (L1) counting.
Results: EPG was lower in Feb treated groups (43.76±1.73) than Alb treated groups (65.51±1.4) and control group (93.96±0.76). There was a significant difference between percentage of the hatched eggs and both treated and control groups. LC50 demonstrated resistance to Alb in treated groups; while it uncovered suspicion to drug resistance in Feb treated groups.
Conclusion: It was concluded that there was resistance to Alb and suspected resistance to Feb in sheep examined.
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Type of Study: Research | Subject: انگل و قارچ شناسی

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