Volume 31, Issue 12 (March 2021)                   Studies in Medical Sciences 2021, 31(12): 921-926 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ebrahimi R, Yakhchali M, Malekinejad H. IN-VITRO ASSESSMENT OF RESISTANCE TO ALBENDAZOLE AND FENBENDAZOLE IN GASTROINTESTINAL NEMATODES. Studies in Medical Sciences 2021; 31 (12) :921-926
URL: http://umj.umsu.ac.ir/article-1-4921-en.html
Professor, Department of Pathobiology, Parasitology Division, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. (Corresponding Author) , m.yakhchali@urmia.ac.ir
Abstract:   (2046 Views)
Background & Aims: Drug resistance is a great concern worldwide and in Iran. This study was carried out to assess drug resistance of gastrointestinal nematodes to Albendazole (Alb) and Fenbendazole (Feb) in sheep.
Materials & Methods: A total number of 90 fresh fecal samples were directly collected from the rectum of infected sheep with an average egg per gram of feces (EPG)≤150. A dilution of 1000 eggs per 2mL was added to each well of control, Alb (0.1µg/ml) and Feb (0.1µg/ml) groups and incubated. To determine drug resistance, a lethal dose of 50% (LD50) was calculated based on percentage of the hatched eggs and first larvae stage (L1) counting.
Results: EPG was lower in Feb treated groups (43.76±1.73) than Alb treated groups (65.51±1.4) and control group (93.96±0.76). There was a significant difference between percentage of the hatched eggs and both treated and control groups. LC50 demonstrated resistance to Alb in treated groups; while it uncovered suspicion to drug resistance in Feb treated groups.
Conclusion: It was concluded that there was resistance to Alb and suspected resistance to Feb in sheep examined.
Full-Text [PDF 575 kb]   (1160 Downloads)    
Type of Study: Research | Subject: انگل و قارچ شناسی

1. Gholamian A, Eslami A, Nabavi L, Rasekh AR, Galedari H. A field survey on resistance to Albendazole in gastrointestinal nematodes of sheep in Khozestan province of Iran. Journal of Veterinary Research 2007; 62(1): 45-51. (Persian) [Google Scholar]
2. Eslami A. Veterinary helminthology: Nematodes and Acantocephala. Tehran, Iran: University of Tehran;1997. [URL]
3. Coles GC. Anthelmintic resistance in sheep. Vet Clin N Am 1986; 2: 423-32. [DOI:10.1016/S0749-0720(15)31253-6]
4. Taylor M, Hunt K. Anthelmintic drug resistance in the UK. Vet Rec 125: 143-7. [DOI:10.1136/vr.125.7.143] [PMID]
5. Waller PJ. International approaches to the concept of integrated control of nematode parasites of livestock. Int J Parasitol 1999; 29: 155-64. [DOI:10.1016/S0020-7519(98)00178-7]
6. Conder GA, Campbell W. Chemotherapy of nematode infections of veterinary importance with special reference to drug resistance. Adv Parasit 1995; 35(3): 1-8. [DOI:10.1016/S0065-308X(08)60069-X]
7. Taylor MA, Hunt KR, Goodyear KL. Anthelmintic resistance detection methods. Vet Parasitol 2002; 103: 183-94. [DOI:10.1016/S0304-4017(01)00604-5]
8. Amarante AFT, Pomroy WE, Pomroy WP, Leathwick D, Tornero MTT. Evalution of a larval development assay for the detection of anthelmintic resistance in Ostertagia. Int J Parasitol 1997; 27(3): 305-11. [DOI:10.1016/S0020-7519(96)00183-X]
9. Coles GC, Jackson F, Pomroy WE, Prichard RK, Von Samson-Himmelstjerna G, Sylvester A, et al. The detection of anthelmintic resistance in nematodes of veterinary importance. Vet Parasitol 2006; 136: 167-85. [DOI:10.1016/j.vetpar.2005.11.019] [PMID]
10. Hunt KR, Taylor MA. Use of the egg hatch assay on sheep faecal samples for the detection of benzimidazole resistant worms. Vet Rec 1989; 125: 153-4. [DOI:10.1136/vr.125.7.153] [PMID]
11. Coles GC, Bauer C, Borgsteede FHM, Geerts S, Klei TR, Taylor MA, et al. World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P) methods for detection of anthelmintic resistance in nematodes of veterinary importance. Vet Parasitol 1992; 44(1-2): 35-44. [DOI:10.1016/0304-4017(92)90141-U]
12. Gholamian A, Eslami A, Nabavi L, Rasekh AR, Galedari H. A survey on drug resistance in gastrointestinal nematodes of sheep using larval development assay. Iranian Veterinary Journal 2008; 4(3): 48-57. (Persian) [Google Scholar]
13. Bartley DJ, Jackson F, Jackson E, Sargison N. Characterisation of two triple resistant field isolates of Teladorsagia from Scottish lowland sheep farms. Vet Parasitol 2004; 123: 189-99. [DOI:10.1016/j.vetpar.2004.06.018] [PMID]
14. Hosseini SH, Mesghi B, Fattahpur S, Mahdavi A, Nazar Alipour R. Evaluation of trichlabandazole and albendazole drug resistance against Fasciola species in Gilan province. Iranian Veterinary Medicine 2010; 6(4): 29-37. (Persian) [Google Scholar]
15. Kumar SC, Renukaprasad PE, Pradeep BS. Evaluation of Albendazole against sheep strongyles in farms of Karnataka state by different in vitro tests. Indian J Anim Sci 2014; 43(5): 330-9. [Google Scholar]
16. Maingi N. Resistance to thiabendazole, fenbendazole and levamizole in Haemonchus and Trichstrongylus species in sheep on a Kenyan farm. Vet Parasitol 1991; 39: 285-91. [DOI:10.1016/0304-4017(91)90045-W]
17. Martin RJ. Modes of action anthelmintic drug. Vet J 1997; 154(1): 11-34. [DOI:10.1016/S1090-0233(05)80005-X]
18. Papadopoulos E, Himonas C, Coles GC. Drought and flock isolation may enhance the development of anthelmintic resistance in nematodes. Vet Parasitol 2001; 97: 253-9. [DOI:10.1016/S0304-4017(01)00435-6]
19. Deepa CK, Devada K. In vitro detection of benzimidazole resistance of gastrointestinal nematodes in goats. J Anim Sci 2011; 42: 73-5.
20. Rialch A, Vatsya S, Kumar RR. Detection of benzimidazole resistance in gastrointestinal nematodes of sheep and goats of sub-Himalyan region of northern India using different tests. Vet Parasitol 2013; 198: 312-8. [DOI:10.1016/j.vetpar.2013.09.018] [PMID]
21. Singh S, Yadav CL. A Survey of anthelmintics resistance by nematodes by three sheep and two goat farms in Hisar (India). Vet Res Commun 1997; 21: 447-51. [DOI:10.1023/A:1005811503644] [PMID]
22. Varady M, Cernanska D, Corba J. Use of two in vitro methods for the detection of anthelmintic resistant nematode parasites on Slovak sheep farms. Vet Parasitol 2006; 135: 325-31. [DOI:10.1016/j.vetpar.2005.10.006] [PMID]
23. Chartier C, Pors I, Hubert J, Rocheteau D, Benoit C, Bernard N. Prevalence of anthelmintic resistant nematodes in sheep and goats in Western France. Small Ruminant Res 1998; 29: 33-41. [DOI:10.1016/S0921-4488(97)00116-8]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Studies in Medical Sciences

Designed & Developed by : Yektaweb