Received: 1 May , 2013 Accepted: 2 Jul , 2013
Abstract
Background & Aims: Brucellosis is a
worldwide zoonotic disease that caused by Brucella spp.
They are facultative intracellular pathogens which replicate inside macrophages
and in non professional phagocytes cells.
The experiences show that medicinal plants can be used
successfully to treat bacterial diseases. The aim of this study is evaluation of bactericidal
effects of Eucalyptus globulus extracts on intramacrophage Brucella
abortus S99.
Material & Methods: In this study aquatic,
ethanolic and acetonic extracts of Eucalyptus globulus leaves prepared.
Then effect of extracts on intramacrophge surviving of B. abortus S99 studied
on cell culture of Balb/c mice peritoneal macrophages. Therefore, after lysis of macrophages, preparation of serial dilution and
culture on Muller-Hinton Agar media, number of grown CFU counted.
Results: Results indicated that Eucalyptus globulus extracts
have antimicrobial activity on intramacrophage B. abortus S99 after 24h.
Conclusion: Overall cell culture results indicated that Eucalyptus
globulus extracts have bactericidal activity on intramacrophage
B. abortus S99 and ethanolic extract
has the most effective antimicrobial activity on
intramacrophage brucellae.
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