Volume 31, Issue 1 (April 2020)                   Studies in Medical Sciences 2020, 31(1): 7-14 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

URL: http://umj.umsu.ac.ir/article-1-4596-en.html
Department of Medicinal Chemistry, School of Pharmacy, Urmia University of Medical Sciences, Urmia, Iran (Corresponding Author) , mrvardast@gmail.com
Abstract:   (3080 Views)
Background & Aims: Amiodarone is an anti-arthritis drug (III) used to treat various types of arrhythmias. Determining the concentration of this drug in the blood of patients is important for the administration of this drug and its interaction with other drugs (due to its high interaction with other drugs). It is necessary to use precision instruments such as HPLC with expensive detectors, but the analysis is often time-consuming and the methods of extraction have a high cost. Therefore, the liquid-liquid extraction method for rapid and accurate measurement of this medication was used in serum.
Materials & Methods: The liquid-liquid extraction method was used with a variety of solvent extraction and different types of solvent. After selecting suitable solvents and optimizing various experimental parameters, this method was used to extract and measure the concentration of amiodarone in Blood serum.
Results: The results showed that carbon tetrachloride is suitable as a solvent extraction and acetonitrile is suitable as a solvent, and their optimal volume is 75 and 200 μl, respectively, and the optimal volume of the serum is 100 μl and the appropriate pH is in the two extraction steps 1 and 6. Mean calibration curve of Amiodarone is linear in the range of 0.05-0.0 μg / ml and has a relative standard deviation of 3.79%.
Conclusion: The results indicate that this method is suitable for measuring amiodarone in blood serum and reduces the time and cost of the analysis to a satisfactory level and has a good repeatability. With this method, it is easy to measure the effect of serum tissue in measuring amiodarone.
Full-Text [PDF 1742 kb]   (1029 Downloads)    
Type of Study: Research | Subject: داروسازی

1. Pena Arranz M.I, Moro C. Amiodarone Determination by high performance liquidchromatography. J Pharm Biomed Anal 1989; 7: 1909-13. [DOI:10.1016/0731-7085(89)80213-4] [PMID]
2. James J, Heger MD, Elizabeth B, Solow MS. Plasma and Red Blood Cell Concentrations of Amiodarone during Chronic Therapy. Am J Cardiol 1984; 53: 912-17. [DOI:10.1016/0002-9149(84)90524-1] [PMID]
3. Eskes S.A, Wiersinga W.M. Amiodarone and thyroid. Best Pract Res Clin Endocrinol Metab 2009; 23: 735-51. [DOI:10.1016/j.beem.2009.07.001] [PMID]
4. Pérez-Ruiz T, Martinez-Lozano C, Martín J.Simultaneous determination of amiodarone and its metabolite desethylamiodarone by high-performance liquid chromatography with chemiluminescent detection. Anal Chim Acta 2008; 623: 89-95. [DOI:10.1016/j.aca.2008.06.003] [PMID]
5. Kuhn J, Tting C, Kleesiek K. Simultaneous measurement of amiodarone and desethylamiodarone in human plasma and serum by stable isotope dilution liquid chromatography-tandem mass spectrometry assay. J Pharm Biomed Anal 2010; 51: 210-6. [DOI:10.1016/j.jpba.2009.08.004] [PMID]
6. Ge D, Kee Lee H. Ionic liquid based dispersive liquid-liquid microextraction coupled with micro-solid phase extraction of antidepressant drugs from environmental water samples. J Chromatogr A2013; 1317: 217- 22. [DOI:10.1016/j.chroma.2013.04.014] [PMID]
7. Usama A, Nilgun G, Gog˘er N.E. Dispersive liquid-liquid microextraction combined with field-amplified sample stacking in capillary electrophoresis for the determination of non-steroidal anti- inflammatory drugs in milk and dairy products. Food Chem 2013; 138: 890-7. [] [PMID]
8. Chaomei X, Jinlan R, Yaling C, Ying T. Extraction and determination of some psychotropic drugs in urine samples using dispersive liquid-liquid microextraction followed by high-performance liquid Chromatography. J Pharm Biomed Anal 2009; 49: 572-8. [DOI:10.1016/j.jpba.2008.11.036] [PMID]
9. Bolderman R.W, Hermans J.J.R, Maessen J.G. Determination of the class III antiarrhythmic drugs dronedarone and amiodarone, and their principal metabolites in plasma and myocardium by HPLC and UV detection. J Chromatogr B 2009; 877(18-19):1727-31. [DOI:10.1016/j.jchromb.2009.04.029] [PMID]
10. Rezaee M, Assadi Y. Determination of organic compounds in water using dispersive liquid-liquid microextraction. J Chromatogr A 2006; 1116: 1-9. [DOI:10.1016/j.chroma.2006.03.007] [PMID]
11. Berijani S, Assadi Y. Dispersive liquid-liquid microextraction combined with gas chromatography-flame photometric detection Very simple, rapid and sensitive method for the determination of organophosphorus pesticides in water. J Chromatogr A 2006; 1123: 1-9. [DOI:10.1016/j.chroma.2006.05.010] [PMID]
12. Fattahi N, Samadi S, Assadi Y. Solid-phase extraction combined with dispersive liquid-liquid Microextraction-ultra preconcentration of chlorophenols in aqueous samples. J Chromatogr A 2007; 1169: 63-9. [DOI:10.1016/j.chroma.2007.09.002] [PMID]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Studies in Medical Sciences

Designed & Developed by : Yektaweb