Volume 35, Issue 7 (10-2024)
Studies in Medical Sciences 2024, 35(7): 545-554 |
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Moshtaghie S A, Shirani A, Fouladgar M. THE PROTECTIVE EFFECTS OF ZINC ON THE ACTIVITY OF LIVER ENZYMES IN RATS POISONED WITH ALUMINUM. Studies in Medical Sciences 2024; 35 (7) :545-554
URL: http://umj.umsu.ac.ir/article-1-6248-en.html
URL: http://umj.umsu.ac.ir/article-1-6248-en.html
Department of Biology, Flowerjan Branch, Islamic Azad University, Isfahan, Iran (Corresponding Author) , Alimoshtaghie@gmail.com
Abstract: (820 Views)
Background & Aims: Zinc is required for the activity of many enzymes such as alkaline phosphatase. Aluminum, however, has toxic effects on the body by inhibiting the function of some enzymes. In this study, the protective effects of zinc on the activity of gamma-glutamyl transferase, aspartic aminotransferase, alanine aminotransferase, and alkaline phosphatase were investigated in rats poisoned with aluminum.
Materials & Methods: In this experimental study, 60 male rats were divided into 12 groups of 5. Aluminum chloride at concentrations of 1 mg/kg and 5 mg/kg was injected into the rats for 15 (6 groups) and 45 (6 groups) days. Additionally, a combination of zinc chloride and aluminum chloride was injected. Enzyme activity levels were measured by routine methods. Statistical analysis was performed using one-way analysis of variance and post hoc analysis, specifically the LSD test, using SPSS version 20 software. Significance levels were set at p<0.05, p<0.01, and p<0.001.
Results: During the short-term simultaneous injection of 3% zinc chloride and 5% aluminum chloride, a significant decrease in ALT and AST enzyme activity was observed (P<0.05). During both short and long-term simultaneous injections of 3% zinc chloride and 1% aluminum chloride, a significant increase and decrease in the activity of ALT and ALP, respectively, were observed (P<0.05). Furthermore, in the short period of simultaneous injection of 3% zinc chloride and 5% aluminum chloride, there was a significant increase in enzyme activity compared to the control group and a significant decrease compared to the group receiving 5% aluminum chloride (P<0.05). Additionally, in the long-term administration of simultaneous injections of 1% aluminum chloride and 1% zinc chloride, an increase in GGT activity was observed (P<0.05).
Discussion: Based on the results, zinc appears to have the ability to protect the liver against aluminum poisoning. However, further studies with larger sample sizes are needed.
Materials & Methods: In this experimental study, 60 male rats were divided into 12 groups of 5. Aluminum chloride at concentrations of 1 mg/kg and 5 mg/kg was injected into the rats for 15 (6 groups) and 45 (6 groups) days. Additionally, a combination of zinc chloride and aluminum chloride was injected. Enzyme activity levels were measured by routine methods. Statistical analysis was performed using one-way analysis of variance and post hoc analysis, specifically the LSD test, using SPSS version 20 software. Significance levels were set at p<0.05, p<0.01, and p<0.001.
Results: During the short-term simultaneous injection of 3% zinc chloride and 5% aluminum chloride, a significant decrease in ALT and AST enzyme activity was observed (P<0.05). During both short and long-term simultaneous injections of 3% zinc chloride and 1% aluminum chloride, a significant increase and decrease in the activity of ALT and ALP, respectively, were observed (P<0.05). Furthermore, in the short period of simultaneous injection of 3% zinc chloride and 5% aluminum chloride, there was a significant increase in enzyme activity compared to the control group and a significant decrease compared to the group receiving 5% aluminum chloride (P<0.05). Additionally, in the long-term administration of simultaneous injections of 1% aluminum chloride and 1% zinc chloride, an increase in GGT activity was observed (P<0.05).
Discussion: Based on the results, zinc appears to have the ability to protect the liver against aluminum poisoning. However, further studies with larger sample sizes are needed.
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