Volume 35, Issue 3 (June 2024)
Studies in Medical Sciences 2024, 35(3): 192-203 |
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Ethics code: IR-UU-AEC-3/40
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Babadi Akasheh M, Delirazh N, Seyyedi S. EVALUATION OF TOLL-LIKE RECEPTORS (TLRS) AGONISTS-TREATED MOUSE BONE MARROW-DERIVED MESENCHYMAL STEM CELLS AND THEIR CULTURE SUPERNATANT ON NK CELLS’ ACTIVITY AGAINST YAC-1 CELLS. Studies in Medical Sciences 2024; 35 (3) :192-203
URL: http://umj.umsu.ac.ir/article-1-6224-en.html
URL: http://umj.umsu.ac.ir/article-1-6224-en.html
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran (Corresponding Author) , delirezhn@gmail.com
Abstract: (30 Views)
Background & Aims: Mesenchymal stem cells (MSCs) are multipotent, non-hematopoietic precursor cells that can be found in many adult tissues. The multipotency and immunomodulatory potential of MSCs make these cells a remarkable tool for the treatment of some diseases. It seems that stimulation of toll-like receptors expressed on the surface of mesenchymal stem cells may potentiate the immunomodulatory potential of these cells. This study was conducted to investigate the effects of polarized bone marrow-derived mesenchymal stem cells from mice on the cytotoxic activity of natural killer (NK) cells.
Materials & Methods: MSCs were isolated from the bone marrow of the femur and tibia of NMRI mice. The third passage of cells was treated with LPS and Poly I-C, then the effects of polarized MSCs, as well as their culture supernatant, on the cytotoxic activity of NK cells against lymphoid cancer cells Yac-1 (as target cells), were evaluated using flow cytometry after 12, 24, and 72 hours.
Results: MSCs treated with LPS and Poly I-C, and their respective culture supernatants, decreased the percentage of dead cells (necrosis and apoptosis) (38% and 35% respectively) compared to untreated MSCs (44%). In the case of cell viability, this effect was the opposite; that is, the untreated cells showed higher viability.
Conclusions: Previous studies indicated that MSCs inhibit the expansion and cytotoxicity of NK cells on tumor cells; however, our results revealed that MSCs treated with LPS potentiated the inhibitory effects of MSCs on NK cell activity.
Materials & Methods: MSCs were isolated from the bone marrow of the femur and tibia of NMRI mice. The third passage of cells was treated with LPS and Poly I-C, then the effects of polarized MSCs, as well as their culture supernatant, on the cytotoxic activity of NK cells against lymphoid cancer cells Yac-1 (as target cells), were evaluated using flow cytometry after 12, 24, and 72 hours.
Results: MSCs treated with LPS and Poly I-C, and their respective culture supernatants, decreased the percentage of dead cells (necrosis and apoptosis) (38% and 35% respectively) compared to untreated MSCs (44%). In the case of cell viability, this effect was the opposite; that is, the untreated cells showed higher viability.
Conclusions: Previous studies indicated that MSCs inhibit the expansion and cytotoxicity of NK cells on tumor cells; however, our results revealed that MSCs treated with LPS potentiated the inhibitory effects of MSCs on NK cell activity.
Type of Study: Research |
Subject:
ایمونولوژی
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