Volume 34, Issue 4 (7-2023)
Studies in Medical Sciences 2023, 34(4): 206-214 |
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Ethics code: IR.IAU.NAJAFABAD.REC. 1401.153
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Moshtaghie A, Shirani A, Fouladgar M. COMPARATIVE EFFECTS OF MAGNESIUM AND SELENIUM ELEMENTS ON GAMMA-GLUTAMYL TRANSFERASE, ASPARTIC AMINOTRANSFERASE, AND ALANINE AMINOTRANSFERASE ACTIVITY IN RATS POISONED WITH LEAD IN THE SHORT AND LONG TERMS. Studies in Medical Sciences 2023; 34 (4) :206-214
URL: http://umj.umsu.ac.ir/article-1-5987-en.html
URL: http://umj.umsu.ac.ir/article-1-5987-en.html
Department of Biology, Flowerjan Branch, Islamic Azad University, Isfahan, Iran (Corresponding Author) , alimoshtaghie@gamil.com
Abstract: (1776 Views)
Background & Aim: Lead is one of the heavy element and toxic metal with wide uses. It may enter the human body through breathing and skin. On the other hand, magnesium and selenium can play a protective role against lead toxicity with their antioxidant effects. In this study, we investigated the protective role of magnesium and selenium elements on the activities of gamma-glutamyl transferase, aspartic aminotransferase, and alanine aminotransferase in rats poisoned with lead in the short and long term.
Materials & Methods: In this experimental study, 60 male rats were divided into 12 groups of 5 each. First, lead was injected into the rats with concentrations of 280mg/kg and 80mg/kg in periods of 15 and 45 days. In the experimental groups, selenium + lead and magnesium + lead salts were injected. The level of enzyme activity was analyzed using one-way ANOVA and Duncan Test by SPSS version 22 software and evaluated with significant levels of P<0.05, P<0.01, and P<0.001.
Results: In both time periods, the simultaneous injection of lead and selenium showed a significant increase in ALT enzyme activity compared to the control group (P values = 0.01 and 0.05, respectively), but no significant changes were observed in AST and GGT enzymes. (P>0.05). In the groups receiving lead and magnesium simultaneous, no significant changes in ALT and AST enzymes activity were observed compared to the control (p>0.05). In the long term, in the groups receiving lead and magnesium, a decrease in AST activity alone was observed (p<0.001). In the same period, in the groups receiving selenium, a decrease in GGT activity alone was observed (P<0.05).
Discussion: According to the obtained results, it is expected that magnesium has the ability to protect the liver against lead poisoning, however, in the case of recipients of selenium along with lead, the effects of poisoning exacerbation were observed.
Materials & Methods: In this experimental study, 60 male rats were divided into 12 groups of 5 each. First, lead was injected into the rats with concentrations of 280mg/kg and 80mg/kg in periods of 15 and 45 days. In the experimental groups, selenium + lead and magnesium + lead salts were injected. The level of enzyme activity was analyzed using one-way ANOVA and Duncan Test by SPSS version 22 software and evaluated with significant levels of P<0.05, P<0.01, and P<0.001.
Results: In both time periods, the simultaneous injection of lead and selenium showed a significant increase in ALT enzyme activity compared to the control group (P values = 0.01 and 0.05, respectively), but no significant changes were observed in AST and GGT enzymes. (P>0.05). In the groups receiving lead and magnesium simultaneous, no significant changes in ALT and AST enzymes activity were observed compared to the control (p>0.05). In the long term, in the groups receiving lead and magnesium, a decrease in AST activity alone was observed (p<0.001). In the same period, in the groups receiving selenium, a decrease in GGT activity alone was observed (P<0.05).
Discussion: According to the obtained results, it is expected that magnesium has the ability to protect the liver against lead poisoning, however, in the case of recipients of selenium along with lead, the effects of poisoning exacerbation were observed.
References
1. Sadeghi Hashjin G, Dehravie M, Arab HA, Mohammadyar L. Effects of choronic lead poisoning on risky behaviors in mice. J Shaheed Sadoghi Univ Med Sci2010;18(3): 159-63 (Persian) [URL]
2. Moshtaghie AA, Ani M, Mirhashemi SM. Comparative effects of lead on serum, liver and brain high molecular weight alkaline phosphatase in rats. Pak J Biol Sci 2006;9(12): 2278-82 [DOI:10.3923/pjbs.2006.2278.2282]
3. Mokhtari M, Shariati M, Gashmardi N. The effect of oral lead on the concentration of thyroid hormones and liver enzymes in rats. Hormozgan Med J 1386;11(2): 115-20 (Persian) [Google Scholar]
4. Garmabi B, Jafarisani M, Khastar H. Improvement of renal oxidative stress and function in lead acetate- induced renal toxicity in rats followed by ascorbic acid consumption. J Knowledge Health 2019;14(2): 33-7 (Persian) [Google Scholar]
5. Fathi F, Mehrpour M, Arefi Oskoui A. investigating the role of serum selenium in parkinsons disease. Res Med 1392;37(2): 90-2 (persian) [URL]
6. Moshtaghie AA. modern clinical biochemistry. isfahan: academic jihad (2017).
7. Aminipor M, Barkhordari Firouz Abadi A, Ehram Poush Mh, Hakiman Am.Determining blood lead concentration of lead and zinc mine works. J Med Res 1999;16(2)24-30
8. Wani AL, Ara A, Usmani JA. Interdiscipline Toxicol Pub Online 2015;8(2)55-64 [DOI:10.1515/intox-2015-0009] [PMID] [PMCID]
9. Akbaeadeh S, Aani M, Moshtaghi AA, Et Al. Effects of magnesium on erythrocyte sodium-lithium countertransport and some of plasma biochemical parameters in rabbit. Iran South Med J 2009;11(2): 116-22 (Persian) [Google Scholar]
10. Ramezan Zadeh Late Roudi T, Moshtaghi AA. Comparative effects of lead and magnesium on serom parameters related to kidney function in male wistar rats. Iranian Congress Of Trace Elements Hamadan-Iran. 2005;22(1): 107 (Persian)
11. Moradi F, Eidi A, Mortazavi P. Antioxidant effects of magnesium in reducing oxidative stress by injected via an intraperitoneally carbon tetrachloride in the wistar male adult rats. Yumsj 2014;19(8): 91 (persian) [URL]
12. Ebrahmi Daryani N, Taher M, Shirzad S. Liver enzyme disorder. J Med Organ Islam Rep Iran 2002;30(1): 119.
13. Nouri S, Zoghi A, Sharifi M R, Et Al. Evaluation of thyroid hormones in patients with lead poisoning. Tehran Univ Med J 2018;76(7): 477-83(Persian) [URL]
14. El-Demerdash F. Antioxidant effect of vitamin e and selenium on lipid peroxidation, enzyme activities and biochemical parameters in rats exposed to aluminum. J Trace Elem Med Biol 2004;18(1): 113-21 [DOI:10.1016/j.jtemb.2004.04.001] [PMID]
15. Shokrzadeh M, Abbasi A, Shadborestan A, Omidi M, Jivad F. The protective role of selenium in preventing tissue damage induced by diazinon in rats. J Mazandaran Univ Med Sci 2013;23(98): 92-8 (Persian) [Google Scholar]
16. Moshtaghi E, Moshtaghi AA, Nayeri H, Investigating the comparative effects of lead and zinc on the serum concentration of parameters related ti liver function in male rats. Iranian Congress Of Trace Elements Hamadan-Iran. 2005;22(1): 16 (Persian)
17. Hajivandi A, Movahed A, Akbarzadeh S. Investigating the histochemical effects of lead acetate on rat liver. Iran South Med J 2010 (Persian)
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