Volume 32, Issue 10 (January 2022)                   Studies in Medical Sciences 2022, 32(10): 723-736 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

URL: http://umj.umsu.ac.ir/article-1-5619-en.html
Associate Professor, Department of Biology, Faculty of Science, Zand Institute of Higher Education, Shiraz, Iran (Corresponding Author) , ebrahim.hossini@yahoo.com
Abstract:   (1184 Views)
Background & Aims: Diseases caused by high-fat diets and obesity are now recognized as a health problem. The aim of this study was to evaluate effect of aloe Vera hydroalcoholic extract on spermatogenesis parameters and tissue structure of testis of rats fed with a high-fat diet.
Materials & Methods: 40 adult male Wistar rats were randomly divided into 5 groups (control group (Con), control group fed with a high-fat diet (HFD), and experimental groups 1 to 3 (HFD +150 mg aloe Vera, HFD +300 mg aloe Vera, and HFD + 600 mg aloe Vera(. High-fat emulsion and three doses of aloe Vera were administered orally for 60 days. Finally, after dissection and preparation, testicular tissue was examined histopathologically. The number of spermatogonia, spermatocytes, spermatids, Sertoli, and Leydig cells was counted. Data were analyzed by SPSS software and the means were compared using ANOVA and Post hoc Tukey's test at 0.05%.
Results: The results showed a significant decrease in mean of spermatogonia, spermatocytes, spermatids, and Leydig cells in the group receiving a high-fat diet (HFD) compared to the control group. Aloe Vera extract significantly increased the number of sex cells in the testis. Increasing the dose of aloe Vera to 600 mg/kg further improved histopathological tissue damage.
Conclusion: The results of study showed that the hydroalcoholic extract of aloe Vera in mice fed with a high-fat diet significantly reduces the risk of pathological tissue damage in testes and increases fertility by increasing the number of sex cells.
Full-Text [PDF 1443 kb]   (561 Downloads)    
Type of Study: Research | Subject: نفرولوژی

1. Lu YC, Sudirman S, Mao CF, Kong ZL. Glycoprotein from Mytilus edulis extract inhibits lipid accumulation and improves male reproductive dysfunction in high-fat diet-induced obese rats. Biomed Pharmacother 2019;109:369-76. [DOI:10.1016/j.biopha.2018.10.180] [PMID]
2. Higuera-H.F, Reyes-C.E, Gutiérrez-M.M, Budde H, Blanco-C.C, Veras A, et al. Blueberry intake included in hypocaloric diet decreases weight, glucose, cholesterol, triglycerides and adenosine levels in obese subjects. J Funct Foods 2019; 60: 103-11. [DOI:10.1016/j.jff.2019.06.011]
3. Hirako S, Wada N, Kageyama H, Takenoya F, Izumida Y, Kim H, et al. Autonomic nervous system-mediated effects of galanin-like peptide on lipid metabolism in liver and adipose tissue. Sci Rep 2016; 6: 21-8. [DOI:10.1038/srep21481] [PMID] [PMCID]
4. Arosio B, Gagliano N, Fusaro LM, Parmeggiani L, Tagliabue J, Galetti P, et al. Aloe-Emodin quinone pretreatment reduces acute liver injury induced by carbon tetrachloride. Pharmacol Toxicol 2000; 87(5): 229-33. https://doi.org/10.1034/j.1600-0773.2000.d01-79.x [DOI:10.1034/j.1600-0773.2000.pto870507.x] [PMID]
5. Wagner IV, Klöting N, Atanassova N, Savchuk I, Spröte C, Kiess W, et al. Prepubertal onset of obesity negatively impacts on testicular steroidogenesis in rats. Mol Cell Endocrinol 2016; 437: 154-62. [DOI:10.1016/j.mce.2016.08.027] [PMID]
6. Chahardoli M, Mahmoodi M, Hajizadeh M, Khoramdel Azad H, Khoshdel A, Mirzaei M. Effect of Aloe Vera Hydroalcoholic Extract on Blood Glucose, Serum Insulin and the Key Enzymes in Metabolic Pathways of Glycolysis and Gluconeogenesis in Hepatocytes of Type 1 Diabetic Rats. J Rafsanjan Univ Med Sci 2015; 13 (8):669-82 [Google Scholar]
7. Tanoomand S, Hosseini E, Edalatmanesh A. Investigating on Protective Effect of Aloe vera Alcoholic Extract Gel on Serum Levels of Interleukin-1, Interferon Gamma and TGF-β in Experimental Rat Models with Multiple Sclerosis. Electronic Journal of General Medicine 2019;16(2):em122. [DOI:10.29333/ejgm/93482]
8. Yazdani N, Hosseini S E. Protective effect of hydro-alcoholic extract of Aloe Vera gel on enzymes and liver tissue structure of high-fat diet rats. J Gorgan Univ Med Sci 2018; 20 (2):48-54. [Google Scholar]
9. Mehrdoost S, Yaghmaei P, Jafary H, Ebrahim-Habibi A. The therapeutic effects of berberine plus sitagliptin in a rat model of fatty liver disease. Iran J Basic Med Sci 2021;24(4):451-9. [PMID] [PMCID]
10. Johnsen S G. Testicular biopsy score count--a method for registration of spermatogenesis in human testes: normal values and results in 335 hypogonadal males. Hormones 1970; 1(1): 2-25. [DOI:10.1159/000178170] [PMID]
11. Zhang L, Wei J, Duan J, Guo C, Zhang J, Ren L, et al. Silica nanoparticles exacerbates reproductive toxicity development in high-fat diet-treated Wistar rats. J Hazard Mater 2020; 384: 121-36. [DOI:10.1016/j.jhazmat.2019.121361] [PMID]
12. Galarza RA, Rhon-Calderón EA, Bizzozero M, Meneghini MA, Cortez AE, Lux-Lantos VA, et al. Impact of maternal overweight on the sexual maturity of male offspring in rats. J Nutr Biochem 2019;71: 27-34. [DOI:10.1016/j.jnutbio.2019.05.014] [PMID]
13. Ferramosca A, Moscatelli N, Di Giacomo M, Zara V. Dietary fatty acids influence sperm quality and function. Andrology 2017; 5(3): 423-30. [DOI:10.1111/andr.12348] [PMID]
14. Rahoui W, Merzouk H, El Haci E, Bettioui R, Azzi R, Benali M. Beneficial effects of Aloe vera gel on lipid profile, lipase activities and oxidant/antioxidant status in obese rats.J Funct Foods 2018;48: 525-32. [DOI:10.1016/j.jff.2018.07.050]
15. Chokboribal J, Tachaboonyakiat W, Sangvanich P, Ruangpornvisuti V, Jettanacheawchankit S, Thunyakitpisal P. Deacetylation affects the physical properties and bioactivity of acemannan, an extracted polysaccharide from Aloe vera. Carbohydr Polym 2015; 133: 556-66. [DOI:10.1016/j.carbpol.2015.07.039] [PMID]
16. Sharma K, Mittal A, Chauhan N. Aloe Vera as Penetration Enhancer. Int J Drug Dev Res 2015; 7: 31-43. [Google Scholar]
17. Minjares-Fuentes R, Rodríguez-González VM, González-Laredo RF, Eim V, González-Centeno MR, Femenia A. Effect of different drying procedures on the bioactive polysaccharide acemannan from Aloe vera (Aloe barbadensis Miller). Carbohydr Polym 2017; 168: 327-36. [DOI:10.1016/j.carbpol.2017.03.087] [PMID]
18. Tada A, Misawa E, Tanaka M, Saito M, Nabeshima K, Yamauchi K, et al. Investigating Anti-Obesity Effects by Oral Administration of Aloe vera Gel Extract (AVGE): Possible Involvement in Activation of Brown Adipose Tissue (BAT). J Nutr Sci Vitaminol 2020; 66(2): 176-84. [DOI:10.3177/jnsv.66.176] [PMID]
19. Shakib Z, Shahraki N, Razavi BM, Hosseinzadeh H. Aloe vera as an herbal medicine in the treatment of metabolic syndrome:A review. Phytother Res 2019;33(10):2649-60. [DOI:10.1002/ptr.6465] [PMID]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2023 CC BY-NC 4.0 | Studies in Medical Sciences

Designed & Developed by : Yektaweb