Volume 32, Issue 5 (August 2021)
Studies in Medical Sciences 2021, 32(5): 317-328 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Khang nikfarjam S, Baharara J, Nejad shahrokhabady K. CYTOTOXIC EFFECTS OF ARTEMISIA ABSINTHIUM EXTRACT ON A2780 CELL LINE (OVARIAN CANCER) AND ALTERATION OF APOPTOTIC GENES EXPRESSION LEVELS. Studies in Medical Sciences 2021; 32 (5) :317-328
URL: http://umj.umsu.ac.ir/article-1-5552-en.html
URL: http://umj.umsu.ac.ir/article-1-5552-en.html
Islamic Azad University, Mashhad , baharara78@gmail.com
Abstract: (3044 Views)
Background & Aims: Ovarian cancer is the third most common cancer in women. Artemisia is one of the most commonly used medicinal plants, and Artemisia absinthium is one of the important species of this genus. The aim of this study was to evaluate the effect of cytotoxicity and the ability to induce apoptosis methanolic extract of A. absinthium in A2780 cell line (human ovarian cancer).
Materials & Methods: In this experimental study, methanolic extract of A. absinthium was prepared. MTT assay was used to evaluate the cytotoxic effects of A. absinthium extract on A2780 cancer cell proliferation and DAPI staining and flow cytometry analysis were used to evaluate the ability to induce apoptosis in treated cells with A. absinthium extract. Changes in "BAX, Caspase 3,9, p53" genes expression were evaluated by Real-time PCR. Quantitative data were analyzed by one-way ANOVA at a significant level of p<0.05.
Results: The MTT assay results showed that methanolic extract of A. absinthium, inhibits proliferation of ovarian cancer A2780 cells in a concentration-dependent manner (p<0.001***). Morphological observation with DAPI staining and flow cytometry analysis results showed an increased percentage of apoptotic cells. The real-time PCR results showed an increase in the expression of apoptotic genes "BAX, Caspase 3,9, p53" in treatment groups p <0.01**; p<0.001***.
Conclusion: The results of this study showed that the methanolic extract of A. absinthium inhibits the proliferation of A2780 cancer cells and induces apoptosis in these cells.
Materials & Methods: In this experimental study, methanolic extract of A. absinthium was prepared. MTT assay was used to evaluate the cytotoxic effects of A. absinthium extract on A2780 cancer cell proliferation and DAPI staining and flow cytometry analysis were used to evaluate the ability to induce apoptosis in treated cells with A. absinthium extract. Changes in "BAX, Caspase 3,9, p53" genes expression were evaluated by Real-time PCR. Quantitative data were analyzed by one-way ANOVA at a significant level of p<0.05.
Results: The MTT assay results showed that methanolic extract of A. absinthium, inhibits proliferation of ovarian cancer A2780 cells in a concentration-dependent manner (p<0.001***). Morphological observation with DAPI staining and flow cytometry analysis results showed an increased percentage of apoptotic cells. The real-time PCR results showed an increase in the expression of apoptotic genes "BAX, Caspase 3,9, p53" in treatment groups p <0.01**; p<0.001***.
Conclusion: The results of this study showed that the methanolic extract of A. absinthium inhibits the proliferation of A2780 cancer cells and induces apoptosis in these cells.
References
1. Vassilev A, DePamphilis ML. Links between DNA replication, stem cells and cancer. Genes (Basel) 2017;8(2):1-33. [DOI:10.3390/genes8020045] [PMID] [PMCID]
2. Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, et al. Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J Clin 2021;71(3):209-49. [DOI:10.3322/caac.21660] [PMID]
3. Farhood B, Geraily G, Alizadeh A. Incidence and mortality of various cancers in Iran and compare to other countries: A review article. Iran J Public Health 2018;47(3):309-16. [PMID] [PMCID]
4. Ottevanger PB. Ovarian cancer stem cells more questions than answers. Semin Cancer Biol 2017;44:67-71. [DOI:10.1016/j.semcancer.2017.04.009] [PMID]
5. Lisio MA, Fu L, Goyeneche A, Gao ZH, Telleria C. High-grade serous ovarian cancer: Basic sciences, clinical and therapeutic standpoints. Int J Mol Sci 2019;20(4):1-33. [DOI:10.3390/ijms20040952] [PMID] [PMCID]
6. Rezaianzadeh A, Mokhtari AM, Hassanipour S, Maghsoudi A, Dehghani SL, Nazarzadeh M, et al. The age-standardized incidence rate of ovarian cancer in iranian women: A systematic review and meta-analysis. Middle East J Cancer 2018;9(3):171-8. [Google Scholar]
7. Momenimovahed Z, Tiznobaik A, Taheri S, Salehiniya H. Ovarian cancer in the world: Epidemiology and risk factors. Int J Womens Health 2019; 11:287-99. [DOI:10.2147/IJWH.S197604] [PMID] [PMCID]
8. Rasmussen CB, Kjaer SK, Albieri V, Bandera E V, Doherty JA, Høgdall E, et al. Pelvic inflammatory disease and the risk of ovarian cancer and borderline ovarian tumors: A pooled analysis of 13 case-control studies. Am J Epidemiol 2017;185(1):8-20. [DOI:10.1093/aje/kww161] [PMID] [PMCID]
9. Liu J, Bai J, Jiang G, Li X, Wang J, Wu D, et al. Anti-tumor effect of Pinus massoniana bark proanthocyanidins on ovarian cancer through induction of cell apoptosis and inhibition of cell migration. PLoS One 2015;10(11):1-15. [DOI:10.1371/journal.pone.0142157] [PMID] [PMCID]
10. Zhang Y, Zhang Y. Marsdenia tenacissima extract inhibits proliferation and promotes apoptosis in human ovarian cancer cells. Med Sci Monit 2018; 24:6289-97. [DOI:10.12659/MSM.909726] [PMID]
11. Paramee S, Sookkhee S, Sakonwasun C, Na Takuathung M, Mungkornasawakul P, Nimlamool W, et al. Anti-cancer effects of Kaempferia parviflora on ovarian cancer SKOV3 cells. BMC Complement Altern Med 2018;18(1):1-13. [DOI:10.1186/s12906-018-2241-6] [PMID] [PMCID]
12. Mohammad RM, Muqbil I, Lowe L, Yedjou C, Hsu HY, Lin LT, et al. Broad targeting of resistance to apoptosis in cancer. Semin Cancer 2015; 35:78-103. [DOI:10.1016/j.semcancer.2015.03.001] [PMID] [PMCID]
13. Matsuura K, Canfield K, Feng W, Kurokawa M. Metabolic Regulation of Apoptosis in Cancer. Int Rev Cell Mol Biol 2016; 327:43-87. [DOI:10.1016/bs.ircmb.2016.06.006] [PMID] [PMCID]
14. Goldar S, Khaniani MS, Derakhshan SM, Baradaran B. Molecular mechanisms of apoptosis and roles in cancer development and treatment. Asian Pacific J Cancer Prev 2015;16(6):2129-44. [DOI:10.7314/APJCP.2015.16.6.2129] [PMID]
15. Khalid EB, Ayman E, Rahman H, Abdelkarim G, Najda A. Natural products against cancer angiogenesis. Tumor Biol 2016;37(11):14513-36. [DOI:10.1007/s13277-016-5364-8] [PMID]
16. Huang XM, Yang ZJ, Xie Q, Zhang ZK, Zhang H, Ma JY. Natural products for treating colorectal cancer: A mechanistic review. Biomed Pharmacother 2019;117:109142. [DOI:10.1016/j.biopha.2019.109142] [PMID]
17. Zyad A, Tilaoui M, Jaafari A, Oukerrou MA, Mouse HA. More insights into the pharmacological effects of artemisinin. Phyther Res 2018;32(2):216-29. [DOI:10.1002/ptr.5958] [PMID]
18. Kooti W, Servatyari K, Behzadifar M, Asadi-Samani M, Sadeghi F, Nouri B, et al. Effective Medicinal Plant in Cancer Treatment, Part 2: Review Study. J Evidence-Based Complement Altern Med 2017;22(4):982-95. [DOI:10.1177/2156587217696927] [PMID] [PMCID]
19. Aiello P, Sharghi M, Mansourkhani SM, Ardekan AP, Jouybari L, Daraei N, et al. Medicinal plants in the prevention and treatment of colon cancer. Oxid Med Cell Longev 2019; 2019:1-51. [DOI:10.1155/2019/2075614] [PMID] [PMCID]
20. Taleghani A, Emami SA, Tayarani-najaran Z. Artemisia a promising plant for the treatment of cancer. Bioorg Med Chem 2019;28:1-22. [DOI:10.1016/j.bmc.2019.115180] [PMID]
21. Martínez-Díaz RA, Ibáñez-Escribano A, Burillo J, de las Heras L, del Prado G, Agulló-Ortuño MT, et al. Trypanocidal, trichomonacidal and cytotoxic components of cultivated Artemisia absinthium Linnaeus (Asteraceae) essential oil. Mem Inst Oswaldo Cruz 2015;110(5):693-9. [DOI:10.1590/0074-02760140129] [PMID] [PMCID]
22. Shafi G, Hasan TN, Syed NA, Al-Hazzani AA, Alshatwi AA, Jyothi A, et al. Artemisia absinthium (AA): A novel potential complementary and alternative medicine for breast cancer. Mol Biol Rep 2012;39(7):7373-9. [DOI:10.1007/s11033-012-1569-0] [PMID]
23. Choi E, Kim G. Effect of Artemisia species on cellular proliferation and apoptosis in human breast cancer cells via estrogen receptor-related pathway. J Tradit Chinese Med 2013;33(5):658-63. [DOI:10.1016/S0254-6272(14)60038-8]
24. Lian G, Li F, Yin Y, Chen L, Yang J. Herbal extract of Artemisia vulgaris (mugwort) induces antitumor effects in HCT-15 human colon cancer cells via autophagy induction, cell migration suppression and loss of mitochondrial membrane potential. J BUON 2018;23(1):73-8. [Google Scholar]
25. Gordanian B, Behbahani M, Carapetian J, Fazilati M. In vitro evaluation of cytotoxic activity of flower, leaf, stem and root extracts of five Artemisia species. Res Pharm Sci 2014;9(2):91-6. [Google Scholar]
26. Kim J, Jung KH, Yan HH, Cheon MJ, Kang S, Jin X, et al. Artemisia Capillaris leaves inhibit cell proliferation and induce apoptosis in hepatocellular carcinoma. BMC Complement Altern Med 2018;18(1):1-10. [DOI:10.1186/s12906-018-2217-6] [PMID] [PMCID]
27. Kim EJ, Kim GT, Kim BM, Lim EG, Kim SY, Kim YM. Apoptosis-induced effects of extract from Artemisia annua Linné by modulating PTEN/p53/PDK1/Akt/ signal pathways through PTEN/p53-independent manner in HCT116 colon cancer cells. BMC Complement Altern Med 2017;17(1):1-12. [DOI:10.1186/s12906-017-1702-7] [PMID] [PMCID]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
