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Background & Aims: Developing bacterial vaginosis is of high prevalence especially in pregnant women. The most common causes of bacterial vaginosis are anaerobic microorganisms such as Gardnerella vaginalis. The aim of this study was to determine the prevalence of Gardenerella vaginalis isolated in women with bacterial and non-bacterial vaginosis.
Materials & Methods: The study was conducted on 500 women attending to Gynecology Clinic of Imam Hossein Hospital. Demographic data of patients were studied. Based on Amsel criteria, vaginosis was divided into bacterial and non-bacterial vaginosis groups. For diagnosis of Gardnerella vaginalis isolates, the secretion was cultured in specific Colombia-Agar- medium under anaerobic conditions and after gram staining, biochemical tests were performed. Molecular PCR method was used to identify isolates. Data analysis was performed using SPSS software.
Results: Among 220 patients with vaginosis, 56.81% had bacterial and 43% had non bacterial vaginosis. The prevalence of Gardenerella vaginalis was 21.8%. From these, 60.4% and 39.5% were from patients with bacterial and non-bacterial vaginosis, respectively. There was a significant relationship between age group and contraceptive method by IUD with bacterial vaginitis.
Conclusion: The High incidence of Gardenerella vaginalis in women with bacterial vaginosis confirms the important role of bacteria in the occurrence of bacterial vaginosis. It is necessary for identification of pathogenic agents responsible for bacterial vaginal infections using culture-based methods to recommend the correct medical treatment in different societies.

Keywords: Prevalence, Bacterial vaginosis, non bacterial vaginosis, Gardenerella vaginalis

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