Volume 30, Issue 7 (October 2019)                   Stud Med Sci 2019, 30(7): 513-522 | Back to browse issues page

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Shaykh-Baygloo N, Bouzari M. ISOLATION OF LYTIC BACTERIOPHAGE AB72P AGAINST MULTI-DRUG RESISTANT ACINETOBACTER BAUMANNII ISOLATES OBTAINED FROM BURN INFECTION. Stud Med Sci. 2019; 30 (7) :513-522
URL: http://umj.umsu.ac.ir/article-1-4524-en.html
Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran (Corresponding Author) , bouzari@sci.ui.ac.ir
Abstract:   (1926 Views)
Background & Aims: Acinetobacter baumannii is a gram-negative pathogen that causes a wide range of hospital-acquired infections. Due to its intrinsic traits and its remarkable abilities to quickly acquire resistance genes, it has become resistant to most antimicrobial agents and a major problem for hospitals. In recent years, application of lytic bacteriophages has been considered to eradicate multi-drug resistant (MDR) A. baumannii from the clinical setting. The purpose of this research was to isolate a lytic bacteriophage against MDR A. baumannii isolate obtained from an infected burn site.
Materials & Methods: Different samples including lake, river and seawater, soil and municipal wastewater were tested for phage isolation by spot test and agar overlay method.
Results: Lytic bacteriophage Ab72p against MDR A. baumannii isolate was isolated from municipal wastewater sample and classified as a member of Myoviridae family. The plaques formed by lytic phage Ab72p were completely clear plaque and had a diameter of 1-2 mm without halo. Phage Ab72p had an about 11 nm (in diameter) icosahedral head and a 17-22 nm (in length) contractile tail. The genome of phage Ab72p was double-stranded DNA with about 46 kb. Phage Ab72p was a very stable lytic phage in the high temperatures (up to 70 ºC) and wide range of pH (3 to 11), with an appropriate adsorption rate (99% adsorbed within 10 minutes), and large burst size (200 PFU per infected cell).
Conclusion: Phage Ab72p could be appropriate choice to prepare phage cocktail against A. baumannii isolates.
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Type of Study: Research | Subject: ویروس شناسی

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