Volume 34, Issue 5 (August 2023)
Studies in Medical Sciences 2023, 34(5): 259-267 |
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Nasresfahani A, Pashae K, Tavalaee M, Behdarvandiyan P, Hekmatpazhooh Z, Nasr-Esfahani M H. ASSESSMENT OF SPERM DNA INTEGRITY IN OLIGOZOOSPERMIC INDIVIDUALS REFERRED TO ISFAHAN FERTILITY AND INFERTILITY CENTER: A CROSS-SECTIONAL STUDY. Studies in Medical Sciences 2023; 34 (5) :259-267
URL: http://umj.umsu.ac.ir/article-1-5957-en.html
URL: http://umj.umsu.ac.ir/article-1-5957-en.html
Ali Nasresfahani 
, Kosar Pashae , Marziyeh Tavalaee * , Paria Behdarvandiyan , Zahra Hekmatpazhooh , Mohammad Hossein Nasr-Esfahani
, Kosar Pashae , Marziyeh Tavalaee * , Paria Behdarvandiyan , Zahra Hekmatpazhooh , Mohammad Hossein Nasr-Esfahani
Associate Professor, Royan Research Institute, Jihad University Biotechnology Research Center, Reproductive Medicine Research Center, Department of Animal Biotechnology, Isfahan, Iran (Corresponding Author) , Tavalaee.m@gmail.com
Abstract: (979 Views)
Background & Aims: Oligozoospermia or reduced sperm concentration is often associated with abnormal motility and morphology, reflecting abnormal spermatogenesis in the testes. The sperm DNA integrity plays an important role in embryo development, and fertility. Therefore, we aimed to assess sperm DNA integrity in a population of oligozoospermic and normozoospermic men.
Materials & Methods: In this cross-sectional study, 967 oligozoospermic samples (sperm count lower than 39 million per ejaculation), and 967 normozoospermic samples according to World Health Organization criteria were included. Sperm DNA damage was assessed by SCSA and TUNEL assays. Statistical analyzes were performed using SPSS version 22 software and a significance level of less than 0.05 was considered.
Results: Mean sperm DNA damage and also DNA stainability were significantly higher in oligozoospermic individuals than them in normozoospermic individuals (Ps<0.001). Unlike semen volume and sperm count which was significantly lower (P<0.001), the mean of sperm abnormal morphology was significantly higher in oligozoospermic men compared to normozoospermic men (P<0.001).
Conclusion: In individuals with oligozoospermia, in addition to the count, morphology and motility of sperm can also be abnormal according to the WHO threshold. Also, sperm DNA damage is significantly high, which can indicate abnormal spermatogenesis and epididymal immaturity. Therefore, depending on the severity of the damage, it is preferable to manage therapeutic interventions, such as drug therapies or assisted reproductive techniques, in order to make the best decisions regarding treatment.
Materials & Methods: In this cross-sectional study, 967 oligozoospermic samples (sperm count lower than 39 million per ejaculation), and 967 normozoospermic samples according to World Health Organization criteria were included. Sperm DNA damage was assessed by SCSA and TUNEL assays. Statistical analyzes were performed using SPSS version 22 software and a significance level of less than 0.05 was considered.
Results: Mean sperm DNA damage and also DNA stainability were significantly higher in oligozoospermic individuals than them in normozoospermic individuals (Ps<0.001). Unlike semen volume and sperm count which was significantly lower (P<0.001), the mean of sperm abnormal morphology was significantly higher in oligozoospermic men compared to normozoospermic men (P<0.001).
Conclusion: In individuals with oligozoospermia, in addition to the count, morphology and motility of sperm can also be abnormal according to the WHO threshold. Also, sperm DNA damage is significantly high, which can indicate abnormal spermatogenesis and epididymal immaturity. Therefore, depending on the severity of the damage, it is preferable to manage therapeutic interventions, such as drug therapies or assisted reproductive techniques, in order to make the best decisions regarding treatment.
References
1. McLachlan RI. Approach to the patient with oligozoospermia. J Clin Endocrinol Metab 2013;98(3): 873-80. doi: 10.1210/jc.2012-3650. [DOI:10.1210/jc.2012-3650] [PMID]
2. Cooper TG, Hellenkemper B, Jonckheere J, Callewaert N, Grootenhuis AJ, Kersemaekers WM, et al. Azoospermia: virtual reality or possible to quantify? J Androl 2006;27(4): 483-90. doi: 10.2164/jandrol.05210. [DOI:10.2164/jandrol.05210] [PMID]
3. Song SH, Bak CW, Lim JJ, Yoon TK, Lee DR, Kwon SW. Natural course of severe oligozoospermia in infertile male: influence on future fertility potential. J Androl 2010;31(6): 536-9. doi: 10.2164/jandrol.110.010199. [DOI:10.2164/jandrol.110.010199] [PMID]
4. Avendaño C, Franchi A, Taylor S, Morshedi M, Bocca S, Oehninger S. Fragmentation of DNA in morphologically normal human spermatozoa. Fertil Steril 2009;91(4): 1077-84. doi: 10.1016/j.fertnstert.2008.01.015. [DOI:10.1016/j.fertnstert.2008.01.015] [PMID]
5. Nasr-Esfahani MH, Deemeh MR, Tavalaee M. New era in sperm selection for ICSI. Int J Androl 2012;35(4): 475-84. doi: 10.1111/j.1365-2605.2011.01227.x. [DOI:10.1111/j.1365-2605.2011.01227.x] [PMID]
6. Kullisaar T, Türk S, Kilk K, Ausmees K, Punab M, Mändar R. Increased levels of hydrogen peroxide and nitric oxide in male partners of infertile couples. Andrology 2013;1(6): 850-8. doi: 10.1111/j.2047-2927.2013.00123.x. [DOI:10.1111/j.2047-2927.2013.00123.x] [PMID]
7. Agarwal A, Mulgund A, Sharma R, Sabanegh E. Mechanisms of oligozoospermia: an oxidative stress perspective. Syst Biol Reprod Med 2014;60(4): 206-16. doi: 10.3109/19396368.2014.918675. [DOI:10.3109/19396368.2014.918675] [PMID]
8. Borges E Jr, Zanetti BF, Setti AS, Braga DPAF, Provenza RR, Iaconelli A Jr. Sperm DNA fragmentation is correlated with poor embryo development, lower implantation rate, and higher miscarriage rate in reproductive cycles of non-male factor infertility. Fertil Steril 2019;112(3): 483-490. doi: 10.1016/j.fertnstert.2019.04.029. [DOI:10.1016/j.fertnstert.2019.04.029] [PMID]
9. Aitken RJ, De Iuliis GN, Nixon B. The Sins of Our Forefathers: Paternal Impacts on De Novo Mutation Rate and Development. Annu Rev Genet 2020; 54: 1-24. doi: 10.1146/annurev-genet-112618-043617. [DOI:10.1146/annurev-genet-112618-043617] [PMID]
10. Aitken RJ. Role of sperm DNA damage in creating de-novo mutations in human offspring: the 'post-meiotic oocyte collusion' hypothesis. Reprod Biomed Online 2022;45(1): 109-124. doi: 10.1016/j.rbmo.2022.03.012. [DOI:10.1016/j.rbmo.2022.03.012] [PMID]
11. World Health Organization (2010). WHO laboratory manual for the examination and processing of human semen, 5th ed. World Health. Organization. [URL]
12. World Health Organization. WHO Laboratory Manual for the Examination and Processing of Human Semen. 6th ed. WHO Press; Geneva, Switzerland: 2021.
13. Boitrelle F, Shah R, Saleh R, Henkel R, Kandil H, Chung E, et al. The Sixth Edition of the WHO Manual for Human Semen Analysis: A Critical Review and SWOT Analysis. Life (Basel). 2021;11(12): 1368. doi: 10.3390/life11121368. [DOI:10.3390/life11121368] [PMID] [PMCID]
14. Evenson DP. Sperm chromatin structure assay (SCSA®). Methods Mol Biol 2013;927: 147-64. doi: 10.1007/978-1-62703-038-0_14. [DOI:10.1007/978-1-62703-038-0_14] [PMID]
15. Agarwal A, Allamaneni SS. Sperm DNA damage assessment: a test whose time has come. Fertil Steril 2005;84(4): 850-3. doi: 10.1016/j.fertnstert.2005.03.080. [DOI:10.1016/j.fertnstert.2005.03.080] [PMID]
16. Dai Y, Liu J, Yuan E, Li Y, Shi Y, Zhang L. Relationship Among Traditional Semen Parameters, Sperm DNA Fragmentation, and Unexplained Recurrent Miscarriage: A Systematic Review and Meta-Analysis. Front Endocrinol 2022;12: 802632. doi: 10.3389/fendo.2021.802632. [DOI:10.3389/fendo.2021.802632] [PMID] [PMCID]
17. Chen Q, Li D, Cheng J, Xue L, Li J. Influence of the sperm DNA fragmentation index on the outcome of rescue ICSI and the clinical value of rescue ICSI. Zhong Nan Da Xue Xue Bao Yi Xue Ban 2022;47(1): 63-71. English, Chinese. doi: 10.11817/j.issn.1672-7347.2022.210021. [Google Scholar]
18. Ribas-Maynou J, Novo S, Torres M, Salas-Huetos A, Rovira S, Antich M, et al. Sperm DNA integrity does play a crucial role for embryo development after ICSI, notably when good-quality oocytes from young donors are used. Biol Res 2022; 55(1): 41. doi: 10.1186/s40659-022-00409-y. [DOI:10.1186/s40659-022-00409-y] [PMID] [PMCID]
19. Ribas-Maynou J, Yeste M, Becerra-Tomás N, Aston KI, James ER, Salas-Huetos A. Clinical implications of sperm DNA damage in IVF and ICSI: updated systematic review and meta-analysis. Biol Rev Camb Philos Soc 2021;96(4): 1284-300. doi: 10.1111/brv.12700. [DOI:10.1111/brv.12700] [PMID]
20. Verdi A, Nasr-Esfahani M H, Forozanfar M, Tavalaee M. Correlation of Sperm Parameters With Sperm DNA Damage or Sperm Nucleus Chromatin Status in Oligospermic Individuals Referred to Qom Jihad Daneshgahi Infertility Treatment Center in 2017 (Iran). Qom Univ Med Sci J 2021;15(3): 222-9. [DOI:10.52547/qums.15.3.222]
21. Jerre E, Bungum M, Evenson D, Giwercman A. Sperm chromatin structure assay high DNA stainability sperm as a marker of early miscarriage after intracytoplasmic sperm injection. Fertil Steril 2019;112(1): 46-53. e2. doi: 10.1016/j.fertnstert.2019.03.013. [DOI:10.1016/j.fertnstert.2019.03.013] [PMID]
22. Evenson DP, Djira G, Kasperson K, Christianson J. Relationships between the age of 25,445 men attending infertility clinics and sperm chromatin structure assay (SCSA®®) defined sperm DNA and chromatin integrity. Fertil Steril 2020;114(2): 311-20. doi: 10.1016/j.fertnstert.2020.03.028. [DOI:10.1016/j.fertnstert.2020.03.028] [PMID]
23. Mohammadi Z, Tavalaee M, Gharagozloo P, Drevet JR, Nasr-Esfahani MH. Could high DNA stainability (HDS) be a valuable indicator of sperm nuclear integrity? Basic Clin Androl 2020;30: 12. doi: 10.1186/s12610-020-00110-8. [DOI:10.1186/s12610-020-00110-8] [PMID] [PMCID]
24. Lu JC. High DNA stainability (HDS) should not be recommended as a marker for the detection of sperm DNA damage. Andrologia 2022;54(7): e14442. doi: 10.1111/and.14442. [DOI:10.1111/and.14442]
25. Booze M, Brannian J, Von Wald T, Hansen K, Kasperson K, Evenson DP. High DNA stainability in the SCSA® is associated with poor embryo and lower implantation rate. BioMed Online 2019;39: e3-4.development [DOI:10.1016/j.rbmo.2019.07.011]
28. Thornhill JA, Fanning DM, Davis NF, Ward F, Shamoun O, Brinsden P. Testicular Sperm Extraction and Intracytoplasmic Sperm Injection: Outcomes in a specialist fertility centre. Iran Med J 2015;108(9): 263-5. [Google Scholar]
29. Alkandari MH, Moryousef J, Phillips S, Zini A. Testicular Sperm Aspiration (TESA) or Microdissection Testicular Sperm Extraction (Micro-tese): Which Approach is better in Men with Cryptozoospermia and Severe Oligozoospermia? Urology 2021;154: 164-9. doi: https: //doi.org/10.1016/j.urology.2021.04.037 [DOI:10.1016/j.urology.2021.04.037] [PMID]
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