Volume 32, Issue 6 (September 2021)                   Studies in Medical Sciences 2021, 32(6): 437-447 | Back to browse issues page

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URL: http://umj.umsu.ac.ir/article-1-5511-en.html
PhD in Exercise Physiology, Department of Exercise Physiology, Faculty of Physical Education and Sport Science, Kharazmi University, Tehran, Iran (Corresponding Author) , Saber_niazi@yahoo.com
Abstract:   (2101 Views)
Background & Aims: Achieving the peak of athletic performance by being in environmental conditions such as hypobaric hypoxia and maintaining it by reducing exercise pressure is of great importance for athletes, among which injuries to internal organs such as lung tissue due to these conditions are less considered. The aim of the present study was to evaluate the response of HIF-1α and the rate of bronchial and bronchiole apoptosis in lung tissue of male Wistar rats following reduced exercise load in hypoxic hypoxia.
Materials & Methods: The samples of the present study included 24 male Wistar rats (8 control, 16 experimental), healthy and without disease (4 weeks with a mean weight of 72 9 9 g). The experimental group was kept in hypobaric hypoxia for 3 weeks after 6 weeks of periodic training. Half of the experimental rats performed periodic exercises with less intensity (Taper) during three weeks of exposure to hypobaric hypoxia. To measure HIF-1α levels and bronchial apoptosis and pulmonary bronchioles, lung tissue was removed and assayed. Data were analyzed by one-way analysis of variance.
Results: Findings showed that exposure to hypobaric hypoxia caused a significant increase in HIF-1α and bronchial apoptosis and pulmonary bronchioles (p ≥ 0.05). Taper was also associated with a significant decrease (p ≥ 0.05) in HIF-1α and bronchial apoptosis and lung tissue bronchioles compared to hypobaric hypoxia and high-intensity interval training.
Conclusion: Exposure to hypobaric hypoxia is associated with an increase in HIF-1α and bronchial apoptosis and pulmonary bronchioles, which can be used as a method to reduce HIF-1α and bronchial apoptosis and pulmonary bronchioles.
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Type of Study: Research | Subject: Exercise physiology

1. Lundby C, Millet GP, Calbet JA, Bärtsch P, Subudhi AW. Does 'altitude training'increase exercise performance in elite athletes? Br J Sports Med2012;46(11):792-5. [DOI:10.1136/bjsports-2012-091231] [PMID]
2. Papacosta E, Gleeson M. Effects of intensified training and taper on immune function. Revista Brasileira de Educação Física e Esporte 2013;27(1):159-76. [DOI:10.1590/S1807-55092013005000001]
3. Kuwano K. Epithelial cell apoptosis and lung remodeling. Cell Mol Immunol 2007;4(6):419-29. [Google Scholar]
4. Yeh C-H, Cho W, So EC, Chu C-C, Lin M-C, Wang J-J, et al. Propofol inhibits lipopolysaccharide-induced lung epithelial cell injury by reducing hypoxia-inducible factor-1α expression. Br J Anaesth 2011;106(4):590-9. [DOI:10.1093/bja/aer005] [PMID]
5. Dayan F, Mazure NM, Brahimi-Horn MC, Pouysségur J. A dialogue between the hypoxia-inducible factor and the tumor microenvironment. Cancer Microenvironment 2008;1(1):53-68. [DOI:10.1007/s12307-008-0006-3] [PMID] [PMCID]
6. Semenza GL. Hypoxia-inducible factors in physiology and medicine. Cell 2012;148(3):399-408. [DOI:10.1016/j.cell.2012.01.021] [PMID] [PMCID]
7. Groenman F, Rutter M, Caniggia I, Tibboel D, Post M. Hypoxia-inducible factors in the first trimester human lung. J Histochem Cytochem 2007;55(4):355-63. [DOI:10.1369/jhc.6A7129.2006] [PMID]
8. He X, Shi X, Yuan H, Xu H, Li Y, Zou Z. Propofol attenuates hypoxia-induced apoptosis in alveolar epithelial type II cells through down-regulating hypoxia-inducible factor-1α. Injury 2012;43(3):279-83. [DOI:10.1016/j.injury.2011.05.037] [PMID]
9. Jain A, Doyle DJ. Apoptosis and pericyte loss in alveolar capillaries in COVID-19 infection: choice of markers matters. Intensive Care Med 2020;46(10):1965-6. [DOI:10.1007/s00134-020-06208-x] [PMID] [PMCID]
10. Becker PM, Alcasabas A, Yu AY, Semenza GL, Bunton TE. Oxygen-independent upregulation of vascular endothelial growth factor and vascular barrier dysfunction during ventilated pulmonary ischemia in isolated ferret lungs. Am J Respir Cell Mol Biol 2000;22(3):272-9. [DOI:10.1165/ajrcmb.22.3.3814] [PMID]
11. Clerici C, Planès C. Gene regulation in the adaptive process to hypoxia in lung epithelial cells. Am J Physiol Lung Cell Mol Physiol 2009;296(3):L267-L74. [DOI:10.1152/ajplung.90528.2008] [PMID]
12. Ke Q, Costa M. Hypoxia-inducible factor-1 (HIF-1). Mol Pharmacol 2006;70(5):1469-80. [DOI:10.1124/mol.106.027029] [PMID]
13. Greijer A, Van der Wall E. The role of hypoxia inducible factor 1 (HIF-1) in hypoxia induced apoptosis. J Clin Pathol 2004;57(10):1009-14. [DOI:10.1136/jcp.2003.015032] [PMID] [PMCID]
14. Krick S, Eul BG, Hanze J, Savai R, Grimminger F, Seeger W, et al. Role of hypoxia-inducible factor-1α in hypoxia-induced apoptosis of primary alveolar epithelial type II cells. Am J Respir Cell Mol Biol 2005;32(5):395-403. [DOI:10.1165/rcmb.2004-0314OC] [PMID]
15. Mujika I, Padilla S. Scientific bases for precompetition tapering strategies. Med Sci Sports Exerc 2003;35(7):1182-7. [DOI:10.1249/01.MSS.0000074448.73931.11] [PMID]
16. Mujika I. Intense training: the key to optimal performance before and during the taper. Scand J Med Sci Sports 2010;20(s2):24-31. [DOI:10.1111/j.1600-0838.2010.01189.x] [PMID]
17. yadegari M, riahy S, mirdar S, hamidian G, mosadegh P. Assessment of interleukin-6 level and lung inflammatory cells after high-intensity interval training and stay in hypoxic conditions. EBNESINA 2016; 18 (3):26-36 [Google Scholar]
18. Mirdar S, Niazi S, Gholizadeh Karam A, Azzar R. Effect of High intensity interval training and hypobaric hypoxia on Body weight changes and Endurance performance in Male wistar rats following the tapering program. Journal of Jiroft University of Medical Sciences 2020;6(2):234-43. [Google Scholar]
19. Howley ET, Bassett DR, Welch HG. Criteria for maximal oxygen uptake: review and commentary. Med Sci Sports Exerc 1995;27:1292- 301. [DOI:10.1249/00005768-199509000-00009] [PMID]
20. Yadegari M, Riahy S, Mirdar S, Hamidian G. Effect of the adiantum capillus veneris extract on Bax and Bcl2 apoptotic markers of lung modulation in trained rats and exposed to hypoxic stress. J Med Plants 2018;4(64):162-71. [Google Scholar]
21. Mirdar S, Kazemzadeh Y, Arabzadeh E, Shirvani H, Hamidian G. The effects of tapering with and without ethanolic extract of Nigella sativa on Hypoxia Inducible Factor-1α and exercise-induced bronchial changes. J Mil Med 2019;21(2):131-41. [Google Scholar]
22. Scholz CC, Taylor CT. Targeting the HIF pathway in inflammation and immunity. Curr Opin Pharmacol 2013;13(4):646-53.23. Lundby C, Gassmann M, Pilegaard H. Regular endurance training reduces the exercise induced HIF-1α and HIF-2α mRNA expression in human skeletal muscle in normoxic conditions. Eur J Appl Physiol 2006;96(4):363-9. [DOI:10.1007/s00421-005-0085-5] [PMID]
23. Rundqvist H. Skeletal muscle HIF-1 and exercise. Institutionen för fysiologi och farmakologi/Department of Physiology; 2008. [URL]
24. Piret J-P, Mottet D, Raes M, Michiels C. Is HIF-1α a pro-or an anti-apoptotic protein? Biochem Pharmacol 2002;64(5):889-92. [DOI:10.1016/S0006-2952(02)01155-3]
25. Bucchieri F, Puddicombe SM, Lordan JL, Richter A, Buchanan D, Wilson SJ, et al. Asthmatic bronchial epithelium is more susceptible to oxidant-induced apoptosis. Am J Respir Cell Mol Biol 2002;27(2):179-85. [DOI:10.1165/ajrcmb.27.2.4699] [PMID]
26. Wang Z, Yu K, Hu Y, Su F, Gao Z, Hu T, et al. Schisantherin A induces cell apoptosis through ROS/JNK signaling pathway in human gastric cancer cells. Biochem Pharmacol 2020;173:113673. [DOI:10.1016/j.bcp.2019.113673] [PMID]
27. Kenney WL, Wilmore JH, Costill DL. Physiology of sport and exercise. Human kinetics; 2015. [URL]
28. Armstrong LE, Armstrong LE. Performing in extreme environments. Human kinetics Champaign, IL; 2000. [URL]
29. Saikumar P, Dong Z, Patel Y, Hall K, Hopfer U, Weinberg JM, et al. Role of hypoxia-induced Bax translocation and cytochrome c release in reoxygenation injury. Oncogene 1998;17(26):3401-15. [DOI:10.1038/sj.onc.1202590] [PMID]
30. McClintock DS, Santore MT, Lee VY, Brunelle J, Budinger GS, Zong W-X, et al. Bcl-2 family members and functional electron transport chain regulate oxygen deprivation-induced cell death. Mol Cell Biol 2002;22(1):94-104. [DOI:10.1128/MCB.22.1.94-104.2002] [PMID] [PMCID]
31. Lee S-D, Kuo W-W, Lin JA, Chu Y-F, Wang C-K, Yeh Y-L, et al. Effects of long-term intermittent hypoxia on mitochondrial and Fas death receptor dependent apoptotic pathways in rat hearts. Int J Cardiol 2007;116(3):348-56. [DOI:10.1016/j.ijcard.2006.03.064] [PMID]
32. Roels B, Millet GP, Marcoux C, Coste O, Bentley DJ, Candau RB. Effects of hypoxic interval training on cycling performance. Med Sci Sports Exerc2005;37(1):138-46. [DOI:10.1249/01.MSS.0000150077.30672.88] [PMID]
33. Dong Z, Wang JZ, Yu F, Venkatachalam MA. Apoptosis-resistance of hypoxic cells: multiple factors involved and a role for IAP-2. Am J Pathol2003;163(2):663-71. [DOI:10.1016/S0002-9440(10)63693-0]
34. Souza KL, Gurgul-Convey E, Elsner M, Lenzen S. Interaction between pro-inflammatory and anti-inflammatory cytokines in insulin-producing cells. J Endocrinol 2008;197(1):139-50. [DOI:10.1677/JOE-07-0638] [PMID]

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