Volume 31, Issue 3 (June 2020)                   Studies in Medical Sciences 2020, 31(3): 158-168 | Back to browse issues page

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Matin Homaei H, Farzanegi P. THE EFFECT OF CONTINUOUS TRAINING ON RATE OF CASPASE3, BAX AND BCL-2 PROTEINS IN VASCULAR OF INDUCED POISONING INDUCED BY DIFFERENT DOSES OF HYDROGEN PEROXIDE. Studies in Medical Sciences 2020; 31 (3) :158-168
URL: http://umj.umsu.ac.ir/article-1-5108-en.html
Associate Professor of Sport Physiology, Sports Physiology Department, Tehran Central Branch, Islamic Azad University, Tehran, Iran (Corresponding Author) , hasanmatinhomaee@gmail.com
Abstract:   (2502 Views)
Background & Aims: Apoptosis is organized cellular death, induced by various physiological and pathological factors. Therefore, the purpose of this study is to investigate the effect of continuous training on rate of Caspase3, BAX and BCL-2 proteins in rats vascular by induced poisoning due to various doses of hydrogen peroxide.
Materials & Methods: 40 mature male wistar rats were randomly assigned into 5 groups including 8 rats in each group. The groups were injected by: 1-Intraperitoneal injection of 1mM H2O2, group (H1), 2- Intraperitoneal injection of 2mM H2O2, group(H2), 3-Intraperitoneal injection of 1mM H2O2 with regular exercise, group (H1E), 4- Intraperitoneal injection of 2mM H2O2 with regular exercise, group (H2E), and 5-control group(C).The training groups performed regular training on the treadmill daily for 8 weeks, all of rats after the anaesthesia knocked out and the BAX, BCL-2 and Caspas3 proteins ratio were determined and measured by ELISA technic. The ANOVA Two Way test was used to determine the effect of each of the independent variables on the indicators. Also, the ANOVA One Way test of the Bonfroni follow-up test was used to examine the intergroup of each of the variables. And significance was considered as P≤ 00.05.
Results: Continuous training in the rats showed that the amount of BAX protein did not decrease significantly compared to the treatment group with 1 and 2 mM of hydrogen peroxide. Exercise alone or with injections of 1 and 2 mM of hydrogen peroxide, increased the amount of protein BCL-2, which was not significant and decreased Caspase3, BAX, and BAX / BCL-2 ratios in both groups receiving one and two milliliters of hydrogen peroxide, but only changes in Caspase-3 (P = 0.014) and ratio BAX / BCL-2 (p = 0.0002) was significant in groups receiving 2 mM of hydrogen peroxide (P ≤ 0.05).
Conclusion:Continuous exercises are likely to reduce the apoptosis of endothelial cells by reducing Caspase3, BAX / BCL-2 ratio and increasing BCL-2 which is likely to prevent vascular tissu damage.
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Type of Study: Research | Subject: Exercise physiology

References
1. Hashemi M, Kroczak TJ. Apoptosis and autoimmune disease. Curr Med Chem Anti Inflamm Anti Allergy Agents. 2005;4(4):429-37. [DOI:10.2174/1568014054546281]
2. Sadowski-Debbing K, Coy JF, Mier W, Hug H, Los MJ. Caspases-their role in apoptosis and other physiological processes as revealed by knock-out studies. Arch Immunol Ther Exp. 2002;50(1):19-34. [PMID]
3. Upadhyay D, Panduri V, Ghio A, Kamp DW. Particulate matter induces alveolar epithelial cell DNA damage and apoptosis: role of free radicals and the mitochondria. Am J Respir Cell Mol Biol. 2003;29(2):180-7. [DOI:10.1165/rcmb.2002-0269OC] [PMID]
4. Skommer J, Wlodkowic D, Deptala A. Larger than life: mitochondria and the Bcl-2 family. Leuk Res. 2007;31(3):277-86. [DOI:10.1016/j.leukres.2006.06.027] [PMID]
5. Mokhtari-Zaer A, Ghodrati-Jaldbakhan S, Vafaei AA, Miladi-Gorji H, Akhavan MM, Bandegi AR, et al. Effects of voluntary and treadmill exercise on spontaneous withdrawal signs, cognitive deficits and alterations in apoptosis-associated proteins in morphine-dependent rats. Behav Brain Res. 2014;271:160-70. [DOI:10.1016/j.bbr.2014.05.061] [PMID]
6. Youle RJ, Strasser A. The BCL-2 protein family: opposing activities that mediate cell death. Nat Rev Mol Cell Biol. 2008;9(1):47-59. [DOI:10.1038/nrm2308] [PMID]
7. Yang Y, Jemiolo B, Trappe S. Proteolytic mRNA expression in response to acute resistance exercise in human single skeletal muscle fibers. Eur J Appl Physiol. 2006;101(5):1442-50. [DOI:10.1152/japplphysiol.00438.2006] [PMID]
8. Park K-S, Sedlock DA, Navalta JW. Exercise-induced muscle damage and apoptotic protein expression in immune cells. FASEB J; 2007. [DOI:10.1096/fasebj.21.6.A1345]
9. Nabiyuni M, B p, Y d. effects of honey bee poison on leukemia lymphoblastic cancer cells. journal of Faze Med Sci of Kashan university. 2012;16(2):7-121. [URL]
10. Ghavami S, Hashemi M, Kadkhoda K, Alavian SM, Bay GH, Los M. Apoptosis in liver diseases-detection and therapeutic applications. Med Sci Monit. 2005;11(11):RA337-RA45. [PMID]
11. Peterson JM, Bryner RW, Sindler A, Frisbee JC, Alway SE. Mitochondrial apoptotic signaling is elevated in cardiac but not skeletal muscle in the obese Zucker rat and is reduced with aerobic exercise. Eur J Appl Physiol. 2008;105(6):1934-43. [DOI:10.1152/japplphysiol.00037.2008] [PMID] [PMCID]
12. Favero G, Paganelli C, Buffoli B, Rodella LF, Rezzani R. Endothelium and its alterations in cardiovascular diseases: life style intervention. Biomed Res Int. 2014;2014. [DOI:10.1155/2014/801896] [PMID] [PMCID]
13. Čolak E. New markers of oxidative damage to macromolecules. J Med Biochem. 2008;27(1):1-16. https://doi.org/10.5937/jomb0-24652 [DOI:10.2478/v10011-007-0049-x] [PMID] [PMCID]
14. Cai H. Hydrogen peroxide regulation of endothelial function: origins, mechanisms, and consequences. Cardiol Res. 2005;68(1):26-36. [DOI:10.1016/j.cardiores.2005.06.021] [PMID]
15. Janero DR, Hreniuk D, Sharif HM. Hydroperoxide-induced oxidative stress impairs heart muscle cell carbohydrate metabolism. Am J Physiol Cell Physiol. 1994;266(1):C179-C88. [DOI:10.1152/ajpcell.1994.266.1.C179] [PMID]
16. Khoshtabiat L, Mahdavi M. The role of oxidative stress in proliferation and cell death. Iran J Psychiatry Behav Sci. 2015;25(127):130-45. [URL]
17. Mazzola PN, Terra M, Rosa AP, Mescka CP, Moraes TB, Piccoli B, et al. Regular exercise prevents oxidative stress in the brain of hyperphenylalaninemic rats. Metab Brain Dis. 2011;26(4):291. [DOI:10.1007/s11011-011-9264-8] [PMID]
18. Yazdanparast Chaharmahali B, Azarbayjani MA, Peeri M, Farzanegi Arkhazloo P. Increased Expression of Bax and Bcl2 Apoptosis Biomarkers in the Heart of Old Female Rats After Interval Training and Curcumin Consumption. Jorjani Biomed J. 2018;6(4):40-52. [DOI:10.29252/jorjanibiomedj.6.4.40]
19. Kwon I, Song W, Jang Y, Choi MD, Vinci DM, Lee Y. Elevation of hepatic autophagy and antioxidative capacity by endurance exercise is associated with suppression of apoptosis in mice. Annals of hepatology. 2020;19(1):69-78. [DOI:10.1016/j.aohep.2019.08.010] [PMID]
20. Aboutaleb N, Shamsaei N, Khaksari M, Erfani S, Rajabi H, Nikbakht F. Pre-ischemic exercise reduces apoptosis in hippocampal CA3 cells after cerebral ischemia by modulation of the Bax/Bcl-2 proteins ratio and prevention of caspase-3 activation. J PHysiol Sci. 2015;65(5):435-43. [DOI:10.1007/s12576-015-0382-7] [PMID]
21. Ghajari H, Hosseini SA, Farsi S. The effect of endurance training along with cadmium consumption on Bcl-2 and bax gene expressions in heart tissue of rats. Ann Mil Health Sci Re. 2019;17(1). [DOI:10.5812/amh.86795]
22. Sinha-Hikim I, Sinha-Hikim AP, Shen R, Kim H, French SW, Vaziri ND, et al. A novel cystine based antioxidant attenuates oxidative stress and hepatic steatosis in diet-induced obese mice. Exp Mol Pathol. 2011;91(1):419-28. [DOI:10.1016/j.yexmp.2011.04.009] [PMID] [PMCID]
23. Quadrilatero J, Alway SE, Dupont-Versteegden EE. Skeletal muscle apoptotic response to physical activity: potential mechanisms for protection. Appl Physiol Nutr Metab. 2011;36(5):608-17. [DOI:10.1139/h11-064] [PMID]
24. Neto JCR, Lira FS, Oyama LM, Zanchi NE, Yamashita AS, Batista ML, et al. Exhaustive exercise causes an anti-inflammatory effect in skeletal muscle and a pro-inflammatory effect in adipose tissue in rats. Eur J Appl Physiol. 2009;106(5):697. [DOI:10.1007/s00421-009-1070-1] [PMID]
25. Pagan LU, Damatto RL, Cezar MD, Lima AR, Bonomo C, Campos DH, et al. Long-term low intensity physical exercise attenuates heart failure development in aging spontaneously hypertensive rats. Cell PHysiol Biochem. 2015;36(1):61-74. [DOI:10.1159/000374053] [PMID]
26. Kenney WL, Wilmore JH, Costill DL. Physiology of sport and exercise: Human kinetics; 2015. [URL]
27. Shi J, Bei Y, Kong X, Liu X, Lei Z, Xu T, et al. miR-17-3p contributes to exercise-induced cardiac growth and protects against myocardial ischemia-reperfusion injury. Theranostics. 2017;7(3):664. [DOI:10.7150/thno.15162] [PMID] [PMCID]
28. Kanter M, Aksu F, Takir M, Kostek O, Kanter B, Oymagil A. Effects of low intensity exercise against apoptosis and oxidative stress in Streptozotocin-induced diabetic rat heart. Exp Clin Endocrinol Diabetes. 2017;125(09):583-91. [DOI:10.1055/s-0035-1569332] [PMID]
29. Quindry JC, Miller L, McGinnis G, Kliszczewicz B, Irwin JM, Landram M, et al. Ischemia reperfusion injury, KATP channels, and exercise-induced cardioprotection against apoptosis. Eur J Appl Physiol. 2012;113(3):498-506. [DOI:10.1152/japplphysiol.00957.2011] [PMID] [PMCID]
30. Husain K, Hazelrigg SR. Oxidative injury due to chronic nitric oxide synthase inhibition in rat: effect of regular exercise on the heart. Biochim Biophys Acta Mol Basis Dis. 2002;1587(1):75-82. [DOI:10.1016/S0925-4439(02)00070-4] [PMID]
31. Plant DR, Gregorevic P, Warmington SA, Williams DA, Lynch GS. Endurance training adaptations modulate the redox-force relationship of rat isolated slow‐twitch skeletal muscles. Clin Exp Pharmacol Physiol. 2003;30(1‐2):77-81. [DOI:10.1046/j.1440-1681.2002.03794.x] [PMID]
32. Martinez JE, Taipeiro EdF, Chies AB. Effects of continuous and accumulated exercise on endothelial function in rat aorta. Arq Bras Cardiol. 2017;108(4):315-22. [DOI:10.5935/abc.20170036] [PMID] [PMCID]
33. Guizoni DM, Dorighello GG, Oliveira HC, Delbin MA, Krieger MH, Davel AP. Aerobic exercise training protects against endothelial dysfunction by increasing nitric oxide and hydrogen peroxide production in LDL receptor-deficient mice. J Transl Med. 2016;14(1):213. [DOI:10.1186/s12967-016-0972-z] [PMID] [PMCID]
34. Lu K, Wang L, Wang C, Yang Y, Hu D, Ding R. Effects of high-intensity interval versus continuous moderate‑intensity aerobic exercise on apoptosis, oxidative stress and metabolism of the infarcted myocardium in a rat model. Mol Med Rep. 2015;12(2):2374-82. [DOI:10.3892/mmr.2015.3669] [PMID]
35. Kwak H-B, Song W, Lawler JM. Exercise training attenuates age-induced elevation in Bax/Bcl-2 ratio, apoptosis, and remodeling in the rat heart. FASEB Bioadv. 2006;20(6):791-3. [DOI:10.1096/fj.05-5116fje] [PMID]
36. Lee YI, Cho JY, Kim MH, Kim KB, Lee DJ, Lee KS. Effects of exercise training on pathological cardiac hypertrophy related gene expression and apoptosis. Eur J Appl Physiol. 2006;97(2):216-24. [DOI:10.1007/s00421-006-0161-5] [PMID]
37. Liu W, He W, Li H. Exhaustive training increases uncoupling protein 2 expression and decreases Bcl-2/Bax ratio in rat skeletal muscle. Oxid Med Cell Longev. 2013;2013. [DOI:10.1155/2013/780719] [PMID] [PMCID]
38. Ross MD, Malone E, Florida-James G. Vascular ageing and exercise: focus on cellular reparative processes. Oxid Med Cell Longev. 2016;2016. [DOI:10.1155/2016/3583956] [PMID] [PMCID]
39. Souza RR, de França E, Madureira D, Pontes CC, Santana JO, Caperuto EC. Resistance training improves aortic structure in Wistar rats. Braz J Phys Ther. 2017;21(4):244-50. [DOI:10.1016/j.bjpt.2017.05.001] [PMID] [PMCID]
40. Marsh SA, Coombes JS. Exercise and the endothelial cell. Int J Cardiol Heart Vessel. 2005;99(2):165-9. [DOI:10.1016/j.ijcard.2004.02.005] [PMID]
41. Ribeiro F, Alves AJ, Duarte JA, Oliveira J. Is exercise training an effective therapy targeting endothelial dysfunction and vascular wall inflammation? Int J Cardiol Heart Vessel. 2010;141(3):214-21. [DOI:10.1016/j.ijcard.2009.09.548] [PMID]
42. Kim SY, Lee J. Exercise Training suppresses vascular fibrosis in aging obesity induced rats. J Exerc Nutrition Biochem. 2014;18(2):175. [DOI:10.5717/jenb.2014.18.2.175] [PMID] [PMCID]
43. Anderson EJ, Lustig ME, Boyle KE, Woodlief TL, Kane DA, Lin C-T, et al. Mitochondrial H 2 O 2 emission and cellular redox state link excess fat intake to insulin resistance in both rodents and humans. Eur J Clin Invest. 2009;119(3):573-81. [DOI:10.1172/JCI37048] [PMID] [PMCID]
44. Konopka AR, Asante A, Lanza IR, Robinson MM, Johnson ML, Dalla Man C, et al. Defects in mitochondrial efficiency and H2O2 emissions in obese women are restored to a lean phenotype with aerobic exercise training. Diabetes. 2015;64(6):2104-15. [DOI:10.2337/db14-1701] [PMID] [PMCID]
45. Fischer TW, Kleszczyński K, Hardkop LH, Kruse N, Zillikens D. Melatonin enhances antioxidative enzyme gene expression (CAT, GPx, SOD), prevents their UVR‐induced depletion, and protects against the formation of DNA damage (8‐hydroxy‐2'‐deoxyguanosine) in ex vivo human skin. J Pineal Res. 2013;54(3):303-12. [DOI:10.1111/jpi.12018] [PMID]
46. Zhang L, Liu H, Sun L, Li N, Ding H, Zheng J. Glypican-3 as a potential differential diagnosis marker for hepatocellular carcinoma: a tissue microarray-based study. Acta Histochem. 2012;114(6):547-52. [DOI:10.1016/j.acthis.2011.10.003] [PMID]

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