Volume 31, Issue 7 (October 2020)                   Studies in Medical Sciences 2020, 31(7): 530-538 | Back to browse issues page

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Beheshti S, Nikzamir A, Salami S, Mirfakhraie R, Sirati-Sabet M. THE EFFECT OF QUINACRINE ON THE EXPRESSION OF WNT3A GENE IN MDA-MB 231 AND MCF7 BREAST CANCER CELL LINES. Studies in Medical Sciences 2020; 31 (7) :530-538
URL: http://umj.umsu.ac.ir/article-1-4568-en.html
Associate Professor, Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran (Corresponding Author) , sirati@sbmu.ac.ir
Abstract:   (2988 Views)
Background & Aims: Triple-negative breast cancer cells refer to any breast cancer that does not express the genes for the estrogen, progesterone, and HER2 receptors. The Wnt signaling pathway is important in the development and progression of various types of cancers. Quinacrine, a derivative of 9-aminoacridine, has been shown to inhibit the growth of several types of cancer cells. In this study, we examined the effect of Quinacrine on Wnt3a gene of Wnt signaling pathway in breast cancer cell lines MDA-MB 231 and MCF7. MDA-MB 231 cell line has triple-negative breast cancer cell properties.
Materials & Methods: Breast cancer cell lines, MDA-MB 231 and MCF7, were treated with 0.5 µM Quinacrine for 3 days. The dose was selected using the MTT assay. The expression of Wnt3a gene was quantified by Real-time PCR. Significance of observations was checked by means of appropriate statistical methods using p<0.05 as the level of significance.
Results: Quinacrine did not have a meaningful effect on Wnt3a gene expression on the MDA-MB 231 cell line (p = 0.34) in 0.5 µM concentration for 72 hours, but a decrease in Wnt3a gene expression of 1.3 times was observed in MCF7 cell line (p < 0.05).
Conclusion: The Wnt3a gene is important in the Wnt signaling pathway and the present study demonstrated that Quinacrine could not affect the expression of this gene in the MDA-MB 231 cell line, however in the MCF7 cell line, a decrease in the Wnt3a gene expression was observed.
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Type of Study: Research | Subject: بیوشیمی

References
1. Angahar T. An Overview of Breast Cancer Epidemiology, Risk Factors, Pathophysiology, and Cancer Risks Reduction.MOJ Biol Med 2017;1(4):1-5. [DOI:10.15406/mojbm.2017.01.00019]
2. Shah R, Rosso K, Nathanson SD. Pathogenesis, prevention, diagnosis and treatment of breast cancer. World J Clin Oncol 2014;5(3):283-98. [DOI:10.5306/wjco.v5.i3.283] [PMID] [PMCID]
3. Heer E, Harper A, Escandor N, Sung H, McCormack V, Fidler-Benaoudia MM. Global burden and trends in premenopausal and postmenopausal breast cancer: a population-based study. Lancet Glob Health 2020;8(8):e1027-e37. [DOI:10.1016/S2214-109X(20)30215-1]
4. Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA-Cancer J Clin 2018;68(6):394-424. [DOI:10.3322/caac.21492] [PMID]
5. Boyle P. Triple-negative breast cancer: epidemiological considerations andrecommendations. Ann Oncol 2012;23(suppl 6):7-12. [DOI:10.1093/annonc/mds187] [PMID]
6. Chavez KJ, Garimella SV, Lipkowitz S. Triple negative breast cancer cell lines: one tool in the search for better treatment of triple negative breast cancer. Breast Dis 2010;32(1-2):1-17. [DOI:10.3233/BD-2010-0307] [PMID] [PMCID]
7. COMŞA Ş, Cimpean AM, Raica M. The story of MCF-7 breast cancer cell line: 40 years of experience in research. Anticancer Res 2015;35(6):3147-54. [Google Scholar]
8. Dreesen O, Brivanlou AH. Signaling pathways in cancer and embryonic stem cells. Stem Cell Rev 2007;3(1):7-17. [DOI:10.1007/s12015-007-0004-8] [PMID]
9. Bilir B, Kucuk O, Moreno CS. Wnt signaling blockage inhibits cell proliferation and migration, and induces apoptosis in triple-negative breast cancer cells. J Transl Med 2013;11(1):1-12. [DOI:10.1186/1479-5876-11-280] [PMID] [PMCID]
10. Komiya Y, Habas R. Wnt signal transduction pathways. Organogenesis 2008;4(2):68-75. [DOI:10.4161/org.4.2.5851] [PMID] [PMCID]
11. Lindvall C, Bu W, Williams BO, Li Y. Wnt signaling, stem cells, and the cellular origin of breast cancer. Stem Cell Rev 2007;3(2):157-68. [DOI:10.1007/s12015-007-0025-3] [PMID]
12. Huang H-C, Klein PS. The Frizzled family: receptors for multiple signal transduction pathways. Genome Bio 2004;5(7):1-7. [Google Scholar]
13. Mikels A, Nusse R. Wnts as ligands: processing, secretion and reception. Oncogene 2006;25(57):7461-8. [DOI:10.1038/sj.onc.1210053] [PMID]
14. Jung Y-S, Park J-I. Wnt signaling in cancer: therapeutic targeting of Wnt signaling beyond β-catenin and the destruction complex. Exp Mol Med 2020;52:183-91. [DOI:10.1038/s12276-020-0380-6] [PMID] [PMCID]
15. Jackstadt R, Hodder MC, Sansom OJ. WNT and β-Catenin in Cancer: Genes and Therapy. Annu Rev Cancer Biol 2020;4:177-96. [DOI:10.1146/annurev-cancerbio-030419-033628]
16. He S, Lu Y, Liu X, Huang X, Keller ET, Qian C-N, et al. Wnt3a: functions and implications in cancer. Chin J Cancer 2015;34(3):1-9. [DOI:10.1186/s40880-015-0052-4] [PMID] [PMCID]
17. Eriksson A, Österroos A, Hassan S, Gullbo J, Rickardson L, Jarvius M, et al. Drug screen in patient cells suggests quinacrine to be repositioned for treatment of acute myeloid leukemia. Blood Cancer J 2015;5(4):1-8. [DOI:10.1038/bcj.2015.31] [PMID] [PMCID]
18. Gurova K. New hopes from old drugs: revisiting DNA-binding small molecules as anticancer agents. Future Oncol 2009;5(10):1-28. [DOI:10.2217/fon.09.127] [PMID] [PMCID]
19. Ehsanian R, Van Waes C, Feller SM. Beyond DNA binding-a review of the potential mechanisms mediating quinacrine's therapeutic activities in parasitic infections, inflammation, and cancers. Cell Commun Signal 2011;9(1):1-18. [DOI:10.1186/1478-811X-9-13] [PMID] [PMCID]
20. Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 2001;29(9):1-6. [DOI:10.1093/nar/29.9.e45] [PMID] [PMCID]
21. Lee AV, Oesterreich S, Davidson NE. MCF-7 cells-Changing the course of breast cancer research and care for 45 years. J Natl Cancer Inst 2015;107(7):1-4. [DOI:10.1093/jnci/djv073] [PMID]
22. Angelucci C, Maulucci G, Colabianchi A, Iacopino F, D'Alessio A, Maiorana A, et al. Stearoyl-CoA desaturase 1 and paracrine diffusible signals have a major role in the promotion of breast cancer cell migration induced by cancer-associated fibroblasts. Br J Cancer 2015;112(10):1675-86. [DOI:10.1038/bjc.2015.135] [PMID] [PMCID]
23. Zhan T, Rindtorff N, Boutros M. Wnt signaling in cancer. Oncogene 2017;36(11):1461-73. [DOI:10.1038/onc.2016.304] [PMID] [PMCID]
24. Howe LR, Brown AM. Wnt signaling and breast cancer. Cancer Biol Ther 2004;3(1):36-41. [DOI:10.4161/cbt.3.1.561] [PMID]
25. Wang S-H, Li N, Wei Y, Li Q-R, Yu Z-P. β-catenin deacetylation is essential for WNT-induced proliferation of breast cancer cells. Mol Med Rep 2014;9(3):973-8. [DOI:10.3892/mmr.2014.1889] [PMID]
26. Satapathy SR, Siddharth S, Das D, Nayak A, Kundu CN. Enhancement of cytotoxicity and inhibition of angiogenesis in oral cancer stem cells by a hybrid nanoparticle of bioactive quinacrine and silver: Implication of base excision repair cascade. Mol Pharm 2015;12(11):4011-25. [DOI:10.1021/acs.molpharmaceut.5b00461] [PMID]
27. Gurova KV, Hill JE, Guo C, Prokvolit A, Burdelya LG, Samoylova E, et al. Small molecules thatreactivate p53 in renal cell carcinoma reveal a NF-κB-dependent mechanism of p53 suppression in tumors. P Natl Acad Sci USA 2005;102(48):17448-53. [DOI:10.1073/pnas.0508888102] [PMID] [PMCID]
28. Preet R, Mohapatra P, Mohanty S, Sahu SK, Choudhuri T, Wyatt MD, et al. Quinacrine has anticancer activity in breast cancer cells through inhibition of topoisomerase activity. Int J Oncol 2012;130(7):1660-70. [DOI:10.1002/ijc.26158] [PMID]

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