Volume 35, Issue 9 (December 2024)
Studies in Medical Sciences 2024, 35(9): 717-728 |
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Ethics code: IR.IAU.SARI.REC.1402.235
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Khalilollahi Y, Farajtabar Behrestaq S, Askari B, Pourmahak A. EFFECT OF AEROBIC TRAINING ALONG WITH OMEGA-3 ON IL-17 AND IL-22 IN VISCERAL ADIPOSE TISSUE OF AGED RATS FED WITH A HIGH-FAT DIET. Studies in Medical Sciences 2024; 35 (9) :717-728
URL: http://umj.umsu.ac.ir/article-1-6348-en.html
URL: http://umj.umsu.ac.ir/article-1-6348-en.html
Department of Physical Education and Sport Sciences, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran (Corresponding Author) , farajtabarp@yahoo.com
Abstract: (193 Views)
Background & Aims: Considering the systemic inflammation and disorder in the immune system with increasing age and obesity, as well as the effect of aerobic training and omega-3 in modulating inflammation and immunity, the aim of the present study was to determine the effect of aerobic training and omega-3 on the expression of IL-17 and IL-22 in visceral adipose tissue of elderly rats fed with a high-fat diet.
Materials & Methods: In this experimental study, 40 male Wistar rats (mean weight : 148.82 ± 18.24 grams) were divided into 5 groups: Normal Diet (ND), High-Fat Diet (HFD), High-Fat Diet-Training (HFDT), High-Fat Diet-Omega3 (HFDω3), and High-Fat Diet-Training-Omega3 (HFDTω3). The supplement groups received 1 g of omega-3 (per kg of body weight) orally during the intervention period. An aerobic training program, including running on a treadmill with an intensity of 50-60% oxygen consumption (VO2max), was performed 5 days a week for eight weeks. Forty-eight hours after the research protocol, gene expression levels in fat tissue were measured by the real-time PCR method.
Results: HFD induction was associated with an increase in the expression of IL-17 and IL-22
(p = 0.0001). A significant decrease was observed in IL-17 and IL-22 in HFDT (p = 0.026 and
p = 0.037, respectively), HFDω3 (p = 0.035 and p = 0.044, respectively), and HFDTω3 (p = 0.0001 and p = 0.0001, respectively) compared to HFD; and HFDTω3 compared to both HFDT (p = 0.049 and p = 0.039, respectively) and HFDω3 (p = 0.037 and p = 0.033, respectively).
Conclusion: Aerobic training and omega-3 consumption can reduce visceral adipose tissue IL-17 and IL-22, immune disorders, and inflammation induced by HFD in aged rats. However, the effect of combining exercise and supplementation is better.
Materials & Methods: In this experimental study, 40 male Wistar rats (mean weight : 148.82 ± 18.24 grams) were divided into 5 groups: Normal Diet (ND), High-Fat Diet (HFD), High-Fat Diet-Training (HFDT), High-Fat Diet-Omega3 (HFDω3), and High-Fat Diet-Training-Omega3 (HFDTω3). The supplement groups received 1 g of omega-3 (per kg of body weight) orally during the intervention period. An aerobic training program, including running on a treadmill with an intensity of 50-60% oxygen consumption (VO2max), was performed 5 days a week for eight weeks. Forty-eight hours after the research protocol, gene expression levels in fat tissue were measured by the real-time PCR method.
Results: HFD induction was associated with an increase in the expression of IL-17 and IL-22
(p = 0.0001). A significant decrease was observed in IL-17 and IL-22 in HFDT (p = 0.026 and
p = 0.037, respectively), HFDω3 (p = 0.035 and p = 0.044, respectively), and HFDTω3 (p = 0.0001 and p = 0.0001, respectively) compared to HFD; and HFDTω3 compared to both HFDT (p = 0.049 and p = 0.039, respectively) and HFDω3 (p = 0.037 and p = 0.033, respectively).
Conclusion: Aerobic training and omega-3 consumption can reduce visceral adipose tissue IL-17 and IL-22, immune disorders, and inflammation induced by HFD in aged rats. However, the effect of combining exercise and supplementation is better.
Type of Study: Research |
Subject:
Exercise physiology
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