Volume 31, Issue 5 (August 2020)                   Studies in Medical Sciences 2020, 31(5): 364-371 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

nasiri bari Y, babapuor V, ahmadi A, zendehdel M, akbari G. EVALUATION OF THE EFFECT OF HYPOTAURINE ON IN VITRO FERTILIZATION IN THE OOCYTES OBTAINED OF THE EXPERIMENTAL POLYCYSTIC OVARY SYNDROME IN MICE: AN EXPERIMENTAL STUDY. Studies in Medical Sciences 2020; 31 (5) :364-371
URL: http://umj.umsu.ac.ir/article-1-4922-en.html
Associate Professor, Department of Anatomy, Anatomy and Embryology, Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran (Corresponding Author) , ahmadiabbas36@yahoo.com
Abstract:   (1528 Views)
Back ground & Aims: In this experimental study, the effect of hypotaurine on in vitro fertilization and fertility of oocytes from mice with experimental polycystic ovary syndrome was designed and performed.
Materials & Method: In this experimental study, NMRI mice aged 6 to 8 weeks were used. The mice were divided into two groups, control and experimental PCOS. For induction of experimental PCOS, Estradiol Valerate (100 mg/kg, IP) was injected. Then, for ovulation, the injection was performed first with 7.5 IU PMSG in the volume of 0.1 ml and 46-48 hours later with 7.5 IU hCG in the volume of 0.1 ml. Finally, 0/1, 1 and 10 μM curcumin were added to the culture medium of oocytes of PCOS group and development in different groups were evaluated (P <0.05).
Results: Most of the arrested embryos had a high percentage of lysis and fragmentation and the arrested embryos were type I and II. The addition of hypotaurin reduced the rate of lysis and fragmentation and decreased the percentage of arrested embryos. Lesser arrested embryos were type III (P <0.05). This study showed that 1 μM hypotaurin increased fertilization percentage and 10 μM hypotaurin increased the percentage of two-cell embryos and also increased the percentage of blastocytes (P <0.05).
Conclusion: The results showed that addition of certain concentrations of hypotaurin as an antioxidant, improved and increased the fertilization rate and the percentage of two-cell embryos. It can also be helpful in increasing the percentage of blastocysts.
Full-Text [PDF 2389 kb]   (364 Downloads)    
Type of Study: Research | Subject: فیزیولوژی

References
1. Ndefo UA, Eaton A, Robbinson M. Polycystic ovary syndrome 2013; 38: 336-338. [PMCID]
2. Dumesic DA, Schramm RD, Abbott DH. Early Origins of Polycystic Ovary Syndrome (PCOS). Reprod Fertil Dev 2005; 17:349-360. [DOI:10.1071/RD04092] [PMID]
3. Teede HJ, Misso ML, Deeks AA, Moran LJ, Stuckey BGA, Wong JLA, et al. Assessment and management of polycystic ovary syndrome: summary of an evidence-based guideline. Med J Aust 2011; 195: 65. https://doi.org/10.5694/j.1326-5377.2011.tb04131.x [DOI:10.5694/mja11.10915]
4. Daniel A. Dumesic, Vasantha P, and David H. Abbott. Polycystic Ovary Syndrome and Oocyte Developmental Competence. Obstet Gynecol Surv 2008; 63(1): 39-48. [DOI:10.1097/OGX.0b013e31815e85fc] [PMID] [PMCID]
5. Leila A, Najmeh T, Mansoureh M, Fahimeh RT, and Saeedeh Z. Antioxidants and management of polycystic ovary syndrome in Iran, Jan 2015; 13(1): 1-8. [PMID] [PMCID]
6. Hansen PJ, In Vitro Production of Bovine Embryos. Department of Animal Sciences, University of Florida 2013, Version 10-16. [URL]
7. Chian RC, In-vitro maturation of immature oocytes for infertile women with PCOS. Reprod Biomed Online 2004;8(5):547-52. [DOI:10.1016/S1472-6483(10)61101-7] [PMID]
8. Bansal AK and Bilaspuri GS, Impact of oxidative stress and antioxidants an semen functions. Vet Med Int 2011; ID 686137, 7. [DOI:10.4061/2011/686137] [PMID] [PMCID]
9. Ahmadi A, And Sadrkhanlo R. Study of Antioxidant Effects of Hypotaurine on Reduction of Oxidative Stress in the Development of Embryos of Mice Experienced from in vitro fertilization. Urmia Med J 2010; 21: 377-503. [URL]
10. Carbone MC, Tatone C, Delle SD, Marci R, Caserta D, Colonna R, et al. Antioxidant enzymatic defences in human follicular characterization and age-dependent changes. Mol Hum Reprod 2003; 9: 639-43. [DOI:10.1093/molehr/gag090] [PMID]
11. Kalir A, and Henry H. Biological activity of sulfinic acid derivatives in chemistry of sulphinic acid, esters their derivatives edited by saul. Wiley, New York 1990; P. 665. [DOI:10.1002/9780470772270.ch23]
12. Horst VD, Brand A. Occurrence of hypotaurine and inositol in the reproductive tract of the ewe and its regulation by pregnenolone and progesterone. Nature 1969; 223:67-8. [DOI:10.1038/223067a0] [PMID]
13. Roe AH, Dokras A. The Diagnosis of Polycystic Ovary Syndrome in Adolescents. Rev Obstet Gynecol 2011; 4: 45-51. [PMID] [PMCID]
14. Shannon M, Wang Y. Polycystic ovary syndrome: A common but often unrecognized condition. J Midwifery Womens Health 2012; 57:221-30. [DOI:10.1111/j.1542-2011.2012.00161.x] [PMID]
15. Sushma R, Nazi B, Sumith M, and Vassudha B. Beneficial effect of Curcumin in Letrozole induced polycystic ovary syndrome, science direct 2016; 5(2) 116-22. [DOI:10.1016/j.apjr.2016.01.006]
16. Teruhiko W, Jun-ihi S, Kenkichi I, Yutaka T, Masamichi K, and Yoshihiro H. Effect of Hypotaurine on in Vitro Fertilization and Production of Term Offspring from in Vitro-Fertilized Ova of the Japanese Field Vole, Microtus Montebelli. Biology of Reproduction 1996; 54(3): 625-30. [DOI:10.1095/biolreprod54.3.625] [PMID]
17. Meizel S, Lui CW, Working PK, Mrsny RJ. Taurine and hypotaurine: their effects on motility, capacitation and the acrosome reaction of hamster spermatozoa in vitro and their presence in spermatozoa and reproductive tract fluids of several mammals. Dev Growth Differ 1980; 22:483-94. [DOI:10.1111/j.1440-169X.1980.00483.x]
18. Okezie IA, Barry H, Brigid MH. and John B. The antioxidant action of taurine, hypotaurine and their metabolic precursors. Biochem 1988; 256, 251-5. [DOI:10.1042/bj2560251] [PMID] [PMCID]
19. Mario F, Donatella A, Emanuela O, Alberto B, and Laura P. Oxidation of hypotaurine and cysteine sulphinic acid by peroxynitrite. Biochem 2005; 389. 233-240. [DOI:10.1042/BJ20041696] [PMID] [PMCID]
20. Mario FFG, and Laura P. The protective effect of hypotaurine and cysteine sulphinic acid on peroxynitrite-mediated oxidative reactions. Free Radical Research 2008; 42(4): 320-30. [DOI:10.1080/10715760801999727] [PMID]
21. Donelly ET, Mclure N, and Lewis SE. Glutathione and hypotaurine in vitro: effects on human sperm motility, DNA integrity and production of reactive oxygen species. Mutagenesis 2000; 15(1):61-8. [DOI:10.1093/mutage/15.1.61] [PMID]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2023 CC BY-NC 4.0 | Studies in Medical Sciences

Designed & Developed by : Yektaweb