Volume 31, Issue 5 (August 2020)                   Studies in Medical Sciences 2020, 31(5): 364-371 | Back to browse issues page

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URL: http://umj.umsu.ac.ir/article-1-4922-en.html
Associate Professor, Department of Anatomy, Anatomy and Embryology, Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran (Corresponding Author) , ahmadiabbas36@yahoo.com
Abstract:   (2311 Views)
Back ground & Aims: In this experimental study, the effect of hypotaurine on in vitro fertilization and fertility of oocytes from mice with experimental polycystic ovary syndrome was designed and performed.
Materials & Method: In this experimental study, NMRI mice aged 6 to 8 weeks were used. The mice were divided into two groups, control and experimental PCOS. For induction of experimental PCOS, Estradiol Valerate (100 mg/kg, IP) was injected. Then, for ovulation, the injection was performed first with 7.5 IU PMSG in the volume of 0.1 ml and 46-48 hours later with 7.5 IU hCG in the volume of 0.1 ml. Finally, 0/1, 1 and 10 μM curcumin were added to the culture medium of oocytes of PCOS group and development in different groups were evaluated (P <0.05).
Results: Most of the arrested embryos had a high percentage of lysis and fragmentation and the arrested embryos were type I and II. The addition of hypotaurin reduced the rate of lysis and fragmentation and decreased the percentage of arrested embryos. Lesser arrested embryos were type III (P <0.05). This study showed that 1 μM hypotaurin increased fertilization percentage and 10 μM hypotaurin increased the percentage of two-cell embryos and also increased the percentage of blastocytes (P <0.05).
Conclusion: The results showed that addition of certain concentrations of hypotaurin as an antioxidant, improved and increased the fertilization rate and the percentage of two-cell embryos. It can also be helpful in increasing the percentage of blastocysts.
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Type of Study: Research | Subject: فیزیولوژی

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