Volume 35, Issue 6 (September 2024)
Studies in Medical Sciences 2024, 35(6): 479-491 |
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Research code: د/29726
Ethics code: IR.IAU.MSHD.REC.1402.101
Clinical trials code: این مطالعه کارآزمایی بالینی ندارد
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Pourmohammad M, Khayatzadeh J, Sattari Fard H, Tehranipour M, Rakhshandeh H, Zafar Balanejad S. THE EFFECT OF AQUEOUS EXTRACT OF CROCUS SATIVUS ON THE VEGF AND FGF GENE EXPRESSION IN THE PROCESS OF ANGIOGENESIS OF THE CHORIOALLANTOIC MEMBRANE OF CHICK EMBRYOS. Studies in Medical Sciences 2024; 35 (6) :479-491
URL: http://umj.umsu.ac.ir/article-1-6271-en.html
URL: http://umj.umsu.ac.ir/article-1-6271-en.html
Mohammadreza Pourmohammad 
, Jina Khayatzadeh * , Haniyeh Sattari Fard , Maryam Tehranipour , Hasan Rakhshandeh , Saeedeh Zafar Balanejad
, Jina Khayatzadeh * , Haniyeh Sattari Fard , Maryam Tehranipour , Hasan Rakhshandeh , Saeedeh Zafar Balanejad
Assistant professor, Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran (Corresponding Author) , j.khayatzadeh@mshdiau.ac.ir
Abstract: (284 Views)
Background & Aim: Angiogenesis is the biological process of sprouting new vessels from existing vessels in the tissue. The main factors in the molecular guidance of this process are vascular endothelial growth factors (VEGF) and fibroblast growth factor (FGF). Considering the importance of plant medicine sciences and also, the role of angiogenesis in processes such as wound healing, menstrual cycles, placental growth, and ovulation, the aim of this study was to investigate the effect of aqueous extract of Crocus sativus (as one of the prominent medicinal plants in traditional medicine) on changes in the gene expression of VEGF and FGF in the angiogenesis pathway of the chorioallantoic membrane (CAM) of chick embryos.
Materials & Methods: In this research, 40 Ross fertilized eggs were randomly divided into 4 groups including the control group, laboratory control (PBS), and 2 experimental groups. On the eighth day of incubation (temperature 38 degrees Celsius and humidity 55 to 60 percent), the laboratory control group was treated with normal saline, and the experimental groups were treated with doses of 50 and 100 µg/ml of the aqueous extract of Crocus sativus. On the twelfth day, a photo was taken of the chorioallantoic membrane, and a sample was taken for RNA extraction and cDNA production. The collected data were analyzed using Excel and SPSS 20 statistical software.
Results: The average number of vessels (6.25 ± 0.25) and vessel length (15.58 ± 1.11) in the treated group showed a significant decrease (P < 0.05) compared to the control group. Also, the expression level of VEGF (0.3235) and FGF (0.3875) genes in the treated group was significantly (P < 0.05) lower than the control group.
Conclusion: Aqueous extract of Crocus sativus can reduce the expression of VEGF and FGF genes and the angiogenic process of the chorioallantoic membrane of chicken embryos.
Materials & Methods: In this research, 40 Ross fertilized eggs were randomly divided into 4 groups including the control group, laboratory control (PBS), and 2 experimental groups. On the eighth day of incubation (temperature 38 degrees Celsius and humidity 55 to 60 percent), the laboratory control group was treated with normal saline, and the experimental groups were treated with doses of 50 and 100 µg/ml of the aqueous extract of Crocus sativus. On the twelfth day, a photo was taken of the chorioallantoic membrane, and a sample was taken for RNA extraction and cDNA production. The collected data were analyzed using Excel and SPSS 20 statistical software.
Results: The average number of vessels (6.25 ± 0.25) and vessel length (15.58 ± 1.11) in the treated group showed a significant decrease (P < 0.05) compared to the control group. Also, the expression level of VEGF (0.3235) and FGF (0.3875) genes in the treated group was significantly (P < 0.05) lower than the control group.
Conclusion: Aqueous extract of Crocus sativus can reduce the expression of VEGF and FGF genes and the angiogenic process of the chorioallantoic membrane of chicken embryos.
Type of Study: Research |
Subject:
فارماکولوژی
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