Volume 35, Issue 6 (September 2024)
Studies in Medical Sciences 2024, 35(6): 502-510 |
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Ethics code: IR.UMA.REC.1402.091
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Khodayi Zavyah O, Bolboli L, Siahkohian M, Baghaiee B. THE EFFECT OF HIGH INTENSITY INTERVAL TRAINING (HIIT) AND L-CYSTEINE CONSUMPTION ON THE LEVEL OF COX2 AND IL-1B IN THE HEART TISSUE OF RATS WITH TYPE 2 DIABETES. Studies in Medical Sciences 2024; 35 (6) :502-510
URL: http://umj.umsu.ac.ir/article-1-6245-en.html
URL: http://umj.umsu.ac.ir/article-1-6245-en.html
Professor of Sports Physiology, Department of Sports Physiology, Faculty of Educational Sciences and Psychology, Mohaghegh Ardabili University, Ardabil, Iran (Corresponding Author) , l_bolboli@uma.ac.ir
Abstract: (210 Views)
Background & Aims: Type 2 diabetes is associated with increased inflammation in various tissues, including the heart. Examining the factors related to the increase in inflammation caused by diabetes , and its consequences , can be controlled to some extent through the application of non-invasive therapeutic interventions. The aim of this study was to investigate the effect of high - intensity interval training and L-cysteine consumption on the levels of COX2 and IL-1B in the heart tissue of rats with type 2 diabetes.
Materials & Methods: In this experimental study, 30 male Wistar rats were divided into five control and experimental groups by simple random sampling. After the induction of diabetes in the experimental and diabetic control groups, the intervention groups of exercise and L-cysteine supplement consumption performed intense intermittent exercise and supplement consumption for eight weeks. To determine the difference between groups, one-way analysis of variance and LSD post hoc test were used.
Results: The results of this study showed that the implementation of intense interval training resulted in a significant decrease in IL-1B level (p=0.001) and the use of L-cysteine supplement led to a non-significant decrease (p=0.087). In contrast, intense interval training caused a non-significant increase (p=0.14) in Cox2 levels compared to the diabetic control group in the heart tissue of rats. It seems that the decreasing changes in Cox2 levels in the L-cysteine supplement group worsened the diabetic inflammation conditions.
Conclusion: Performing HIIT for eight weeks led to significant changes in the levels of IL-1B in the heart tissue of rats. Also, single and combined L-cysteine supplementation with this type of exercise did not cause obvious changes in IL-1B and COX-2 levels. One of the main limitations of this study was the lack of simultaneous sampling in all samples.
Materials & Methods: In this experimental study, 30 male Wistar rats were divided into five control and experimental groups by simple random sampling. After the induction of diabetes in the experimental and diabetic control groups, the intervention groups of exercise and L-cysteine supplement consumption performed intense intermittent exercise and supplement consumption for eight weeks. To determine the difference between groups, one-way analysis of variance and LSD post hoc test were used.
Results: The results of this study showed that the implementation of intense interval training resulted in a significant decrease in IL-1B level (p=0.001) and the use of L-cysteine supplement led to a non-significant decrease (p=0.087). In contrast, intense interval training caused a non-significant increase (p=0.14) in Cox2 levels compared to the diabetic control group in the heart tissue of rats. It seems that the decreasing changes in Cox2 levels in the L-cysteine supplement group worsened the diabetic inflammation conditions.
Conclusion: Performing HIIT for eight weeks led to significant changes in the levels of IL-1B in the heart tissue of rats. Also, single and combined L-cysteine supplementation with this type of exercise did not cause obvious changes in IL-1B and COX-2 levels. One of the main limitations of this study was the lack of simultaneous sampling in all samples.
Type of Study: Research |
Subject:
Exercise physiology
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