Volume 34, Issue 10 (January 2023)
Studies in Medical Sciences 2023, 34(10): 632-642 |
Back to browse issues page
Ethics code: IR.IAU.PIAU.REC.1401.005،
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Bayat P, nezhadali M, Esfandeyari B. ASSOCIATION OF RETN GENE POLYMORPHISM WITH SERUM RESISTIN LEVEL, INSULIN RESISTANCE, AND NON-ALCOHOLIC FATTY LIVER DISEASE IN AN IRANIAN POPULATION. Studies in Medical Sciences 2023; 34 (10) :632-642
URL: http://umj.umsu.ac.ir/article-1-6113-en.html
URL: http://umj.umsu.ac.ir/article-1-6113-en.html
Assistant Professor of Biochemistry, Department of Biology, Faculty of Science, Islamshahr Branch, Islamic Azad University, Islamshahr, , ma_nejadali@yahoo.com
Abstract: (308 Views)
Background & Aims: Resistin is an adipokine that is secreted from adipose tissue, causing insulin resistance and fatty liver disease. This study aimed to determine the association of resistin gene rs3745368 single nucleotide polymorphisms (G>A+299) with serum resistin level, insulin resistance index, and non-alcoholic fatty liver disease (NAFLD).
Materials & Methods: This descriptive study was conducted on 160 people (80 healthy participants and 80 patients with NAFLD) who referred to Bo Ali and Amirul Mominin Hospitals in Tehran in 2022. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to determine genotype of resistin gene polymorphism rs1862513. Serum resistin and insulin levels were measured using ELISA (Enzyme-linked immunosorbent assay) method, and other parameters were measured by standard methods. Data were analyzed by SPSS statistical software (version20) using independent t test, Mann-Whitney U test, Kruskal-Wallis, ANOVA Spearman’s Correlation Test, and logistic regression test.
Results: In the present study, significant differences were observed among two groups according to the meanlevels of HDL, triglycerides, insulin, resistin, liver enzymes and variables of age, body mass index, systolic blood pressure and HOMA (Homeostatic model assessment) (P<0.05). Significant correlations were observed between resistin levels with body mass index in healthy group and with triglyceride in patients with NAFLD (P<0.05). In the present study, RENT (Resistin) gene polymorphism rs3745368 had no significant relationship with biochemical and anthropometric variables, resistin level, insulin resistance, and non-alcoholic fatty liver (Ps>0.05).
Conclusion: It appears resistin levels are statistically associated with NAFLD, but RENT rs3745368 polymorphism is not statistically associated with resistin level, NAFLD, and insulin resistance.
Keywords:
Materials & Methods: This descriptive study was conducted on 160 people (80 healthy participants and 80 patients with NAFLD) who referred to Bo Ali and Amirul Mominin Hospitals in Tehran in 2022. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to determine genotype of resistin gene polymorphism rs1862513. Serum resistin and insulin levels were measured using ELISA (Enzyme-linked immunosorbent assay) method, and other parameters were measured by standard methods. Data were analyzed by SPSS statistical software (version20) using independent t test, Mann-Whitney U test, Kruskal-Wallis, ANOVA Spearman’s Correlation Test, and logistic regression test.
Results: In the present study, significant differences were observed among two groups according to the meanlevels of HDL, triglycerides, insulin, resistin, liver enzymes and variables of age, body mass index, systolic blood pressure and HOMA (Homeostatic model assessment) (P<0.05). Significant correlations were observed between resistin levels with body mass index in healthy group and with triglyceride in patients with NAFLD (P<0.05). In the present study, RENT (Resistin) gene polymorphism rs3745368 had no significant relationship with biochemical and anthropometric variables, resistin level, insulin resistance, and non-alcoholic fatty liver (Ps>0.05).
Conclusion: It appears resistin levels are statistically associated with NAFLD, but RENT rs3745368 polymorphism is not statistically associated with resistin level, NAFLD, and insulin resistance.
Keywords:
References
1. Marjani S, Nezhadali M, Hekmat A, Yeganeh MZ. Investigating Visfatin gene Polymorphism rs4730153 with Insulin Resistance and Non-Alcoholic Fatty Liver Diseases in Iranian Population. Iran J Public Health 2022;51(5):1143-51. [DOI:10.18502/ijph.v51i5.9429] [PMID] []
2. Brunt EM, Wong VW, Nobili V, Day CP, Sookoian S, Maher JJ, et al. Nonalcoholic fatty liver disease Nature reviews Disease primers. BMC Med 2015;17;1(1):1-22. [DOI:10.1038/nrdp.2015.80] [PMID]
3. 3] Ehtesham R, Hedayati M. Correlation of vaspin levels with anthropometric and biochemical parameters in patients with Non-alcoholic fatty liver disease in an Iranian population. Yafteh 2021;23(3). [Google Scholar]
4. Tabaeian SP, Mahmoudi T, Rezamand G, Nobakht H, Dabiri R, Farahani H, et al. Resistin gene polymorphism and nonalcoholic fatty liver disease risk. Arq Gastroenterol 2022;14;59:483-7. [DOI:10.1590/s0004-2803.202204000-86] [PMID]
5. Pandey AK, Jalihal U, Pramila MN, Gowda V MN, Vinay MD, Prabhu VMD. Estimation of adiponectin levels in diabetic, non-diabetic fatty liver diseases and healthy control sInternational. Int J Res Med Sci 2015;3(1):140-6. [DOI:10.5455/2320-6012.ijrms20150124]
6. Nezhadali M, Mesbah-Namin SA, Hedayati M, Akbarzadeh M, Najd Hassan Bonab L, Daneshpour MS. Serum adiponectin and cortisol levels are not affected by studied ADIPOQ gene variants: Tehran lipid and glucose study. BMC Endocr Disord 2022;22(1):1. [DOI:10.1186/s12902-022-01020-8] [PMID] []
7. Moayeri M, Nezhadali M, Mohamadi M. Assoociation between fat mass and obesity associated (FTO) rs17817449 gene polymorphism with insulin resistance and obesity in the patients with Non-Alcohlic Fatty Liver Disease. Stud Med Sci 2023;33(12):868-75. [DOI:10.61186/umj.33.12.868]
8. Sayar N, Nezhadali M Hedayati M, Mahdavi M, Akbarzadeh M, Zarkesh M. Correlation of Serum Resistin Level with Insulin Resistance and Metabolic Parameters in Patients with Type 2 Diabetes and Prediabetes. J Adv Biol Biomed Res 2020;10(2):2362-70. [URL]
9. Deeb MM, Dawoud AA, El-Hawy MA, Wasel YF, El Sayed AE. Study of adipocytokines (visfatin and resistin) levels in children with β-thalassemia major and intermedia. Menoufia Med J 2019;32(3):1051. [DOI:10.4103/mmj.mmj_835_17]
10. Kawamura R, Tabara Y, Tsukada A, Igase M, Ohashi J, Yamada R, et al. Genome-wide association study of plasma resistin levels identified rs1423096 and rs10401670 as possible functional variants in the Japanese population. J physiol genom 2016;48:874-88. [DOI:10.1152/physiolgenomics.00040.2016] [PMID]
11. Su KZ, Li YR, Zhang D, Yuan JH, Zhang CS, Liu Y, et al. Relation of circulating resistin to insulin resistance in type 2 diabetes and obesity: a systematic review and meta-analysis. Front physiol 2019;10:1399. [DOI:10.3389/fphys.2019.01399] [PMID] []
12. Park HK, Kwak MK, Kim HJ, Ahima RS. Linking resistin, inflammation, and cardiometabolic diseases. Korean J Intern Med 2017;32:239-47. [DOI:10.3904/kjim.2016.229] [PMID] []
13. Bednarska-Makaruk M, Graban A, Wiśniewska A, Łojkowska W, Bochyńska A, Gugała-Iwaniuk M, et al. Association of adiponectin, leptin and resistin with inflammatory markers and obesity in dementia. Biogerontology 2017;18:561-80. [DOI:10.1007/s10522-017-9701-0] [PMID] []
14. Kumar D, Lee B, Puan KJ, Lee W, Luis BS, Yusof N, et al. Resistin expression in human monocytes is controlled by two linked promoter SNPs mediating NFKB p50/p50 binding and C-methylation. Sci Rep 2019;9:15245. [DOI:10.1038/s41598-019-51592-0] [PMID] []
15. Macaluso FS, Maida M, Petta S. Genetic background in nonalcoholic fatty liver disease: a comprehensive review. World J Gastroenterohl 2015;21(39):11088. [DOI:10.3748/wjg.v21.i39.11088] [PMID] []
16. Zhang LY, Jin YJ, Jin QS, Lin LY, Zhang DD, Kong LL. Association between resistin +299A/A genotype and nonalcoholic fatty liver disease in Chinese patients with type 2 diabetes mellitus. Gene 2013;529:340-4. [DOI:10.1016/j.gene.2013.08.001] [PMID]
17. Asano H, Izawa H, Nagata K, Nakatochi M, Kobayashi M, Hirashiki A, et al. Plasma resistin concentration determined by common variants in the resistin gene and associated with metabolic traits in an aged Japanese population. Diabetologia 2010;53(2):234-46. [DOI:10.1007/s00125-009-1517-2] [PMID]
18. Takhshid MA, Zare Z. Resistin - 420 C/G polymorphism and serum resistin level in Iranian patients with gestational diabetes mellitus. J Diabetes Metab Disord 2015; 14:37. [DOI:10.1186/s40200-015-0165-y] [PMID] []
19. Hosseini, M., Nezhadali, M., & Hedayati, M. Association of vaspin rs2236242 gene polymorphism with serum vaspin level, insulin resistance and diabetes in an Iranian diabetic/pre-diabetic population. J Med Biochem 2021;40(1):33. [DOI:10.5937/jomb0-24671] [PMID] []
20. Banihani SA, Abu-Alia KF, Khabour OF, IAlzoubi KH. Association between Resistin Gene Polymorphisms and Atopic Dermatitis. Biomolecules 2018;8(2):17. [DOI:10.3390/biom8020017] [PMID] []
21. Shen C, Zhao CY, Wang W, Wang YD, Sun H, Cao W, et al. The relationship between hepatic resistin overexpression and inflammation in patients with nonalcoholic steatohepatitis. BMC gastroenterol 2014;14(1):1-8. [DOI:10.1186/1471-230X-14-39] [PMID] []
22. Tiwari S, Paul BN, Kumar S, Chandra A, Dhananjai S, et al. Over expression of resistin in adipose tissue of the obese induces insulin resistance. World J Diabetes 2012;3(7):135. [DOI:10.4239/wjd.v3.i7.135] [PMID] []
23. Boyraz M., Cekmez F, Karaoglu A, Cinaz P, Durak M., Bideci A. Serum adiponectin, leptin, resistin and RBP4 levels in obese and metabolic syndrome children with nonalcoholic fatty liver disease. Biomark Med 2013;7(5):737-45. [DOI:10.2217/bmm.13.13] [PMID]
24. Owecki M, Nikisch E, Miczke A, Pupek-Musialik D, Sowinski J. Serum resistin is related to plasma HDL cholesterol and inversely correlated with LDL cholesterol in diabetic and obese humans. Neuro Endocrinol Lett 2010;31(5):673. [Google Scholar]
25. Suriyaprom K, Tungtrongchitr R, Namjuntra P. Associations of Resistin Levels with Resistin Gene Polymorphism and Metabolic Syndrome in Thais. J Med Biochem 2015;34:170-8. [DOI:10.2478/jomb-2014-0034] [PMID] []
26. Khalil O, Alnahal A, Ghonium M, Fawzy S, Ibrahem M, Raafat N. Does resistin gene polymorphisms+ 299 (G> A) participate in insulin resistance in Egyptian non-obese Type 2 Diabetes. Int J Genomic Med 2014;2(1):1-7. [Google Scholar]
27. Hastuti P, Tasmini T, Utami RF, Riwa MR, Steven S, Sadewa AH. Variation of resistin gene is correlated with insulin resistance in obese people of Indonesia. Open Access Maced J Medical Sci 2019;7(12):1891. [DOI:10.3889/oamjms.2019.456] [PMID] []
28. Altawallbeh G, Khabour OF, Alfaqih MA, Abboud MM, Gharibeh M, Mohammed NA. Association of RETN +299(G>A) polymorphism with type two diabetes mellitus. Acta Biochim Pol 2021;68(1):77-81. [DOI:10.18388/abp.2020_5409] [PMID]
29. Kumar V, Singh J, Bala K, Singh J. Association of resistin (rs3745367) and urotensin II (rs228648 and rs2890565) gene polymorphisms with risk of type 2 diabetes mellitus in Indian population. Mol Biol Rep 2020;47(12):9489-9497. [DOI:10.1007/s11033-020-05991-6] [PMID]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
