Volume 34, Issue 2 (5-2023)
Studies in Medical Sciences 2023, 34(2): 90-97 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Kharrazian M, Malekifard M K , F, Rakhshanpour A, Yakhchali M. INVESTIGATION OF TAENIA SPP EGGS IN DOGS OF URMIA SUBURB WITH USING MULTIPLEX PCR METHODS. Studies in Medical Sciences 2023; 34 (2) :90-97
URL: http://umj.umsu.ac.ir/article-1-5949-en.html
URL: http://umj.umsu.ac.ir/article-1-5949-en.html
Mohammad Kharrazian 
, Mohammad Kharrazian, Farnaz Malekifard * , Alaleh Rakhshanpour , Mohammad Yakhchali
, Mohammad Kharrazian, Farnaz Malekifard * , Alaleh Rakhshanpour , Mohammad Yakhchali
Associate Professor of Parasitology, Urmia University, Urmia, Iran (Corresponding Author) , f.malekifard@urmia.ac.ir
Abstract: (1731 Views)
Background & Aims: Taenia spp. and Echinococcus are important common zoonotic parasites in dogs. Dogs as the most important final hosts were infected with several species of these helminths simultaneously which are morphologically indistinguishable. The aim of the present study was to investigate Taenia spp eggs contamination in dogs of Urmia, Iran, by molecular method.
Materials & Methods: In this study, a total of 246 dog fecal samples were collected from Urmia, northwest of Iran, and examined using a flotation method followed by multiplex PCR for detection and identification of parasites’ eggs.
Results: Taeniid eggs were observed in 12 (4.57%) of samples. Based on Multiplex PCR analysis, 1.62%, 2.03% and 1.21% of samples were infected with Echinococcus granulosus, Tenia spp and simultaneous infection of Tania and E. granulosus were observed, respectively. E. multilocularis was not seen in the samples.
Conclusion: Considering the observation of infection with Echinococcus granulosus and Taenia spp. in this study and their importance as zoonotic parasites, it is recommended to design and implement control programs.
Materials & Methods: In this study, a total of 246 dog fecal samples were collected from Urmia, northwest of Iran, and examined using a flotation method followed by multiplex PCR for detection and identification of parasites’ eggs.
Results: Taeniid eggs were observed in 12 (4.57%) of samples. Based on Multiplex PCR analysis, 1.62%, 2.03% and 1.21% of samples were infected with Echinococcus granulosus, Tenia spp and simultaneous infection of Tania and E. granulosus were observed, respectively. E. multilocularis was not seen in the samples.
Conclusion: Considering the observation of infection with Echinococcus granulosus and Taenia spp. in this study and their importance as zoonotic parasites, it is recommended to design and implement control programs.
Type of Study: Research |
Subject:
انگل و قارچ شناسی
References
1. Robertson ID, Thompson RC. Enteric parasitic zoonoses of domesticated dogs and cats. Microbes Infect 2002;4(8):867-3. [DOI:10.1016/S1286-4579(02)01607-6] [PMID]
2. Cardona GA, Carmena D. A review of the global prevalence, molecular epidemiology and economics of cystic echinococcosis in production animals. Vet Parasitol 2013;192(1-3):10-32. [DOI:10.1016/j.vetpar.2012.09.027] [PMID]
3. McManus DP. Molecular discrimination of taeniid cestodes. Parasitol Int;55:S31-S7. [DOI:10.1016/j.parint.2005.11.004] [PMID]
4. Jiang W, Liu N, Zhang G, Renqing P, Xie F, Li T, et al. Specific detection of Echinococcus spp. from the Tibetan fox (Vulpes ferrilata) and the red fox (V. vulpes) using copro-DNA PCR analysis. Parasitol Res 2012;111:1531-9. [DOI:10.1007/s00436-012-2993-8] [PMID]
5. Trachsel D, Deplazes P, Mathis A. Identification of taeniid eggs in the faeces from carnivores based on multiplex PCR using targets in mitochondrial DNA. Parasitol 2007;134(06):911-20. [DOI:10.1017/S0031182007002235] [PMID]
6. Ziadinov I, Mathis A, Trachsel D, Rysmukhambetova A, Abdyjaparov T, Kuttubaev O et al. Canine echinococcosis in Kyrgyzstan: Using prevalence data adjusted for measurement error to develop transmission dynamics models. Int J Parasitol 2008; 38(10):1179-90. [DOI:10.1016/j.ijpara.2008.01.009] [PMID] [PMCID]
7. Soriano SV, Pierangeli NB, Roccia I, Bergagna HF, Lazzarini LE, Celescinco A et al. A wide diversity of zoonotic intestinal parasites infects urban and rural dogs in Neuquén, Patagonia, Argentina. Vet parasitol 2010;167(1):81-5. [DOI:10.1016/j.vetpar.2009.09.048] [PMID]
8. Miró G, Mateo M, Montoya A, Vela E, Calonge R. Survey of intestinal parasites in stray dogs in the Madrid area and comparison of the efficacy of three anthelmintics in naturally infected dogs. Parasitol Res 2007;100(2):317-20. [DOI:10.1007/s00436-006-0258-0] [PMID]
9. Kohansal MH, Nourian A, Haniloo A, Fazaeli A. Molecular detection of Taenia spp. in dogs' feces in Zanjan Province, Northwest of Iran. Vet World 2017;10(4):445. [DOI:10.14202/vetworld.2017.445-449] [PMID] [PMCID]
10. Rahimi MT, Sarvi S, Daryani A, Sharif M, Ahmadpour E, Shokri A et al. Application of multiplex PCR for the simultaneous detection of spp. from domestic dogs in the north of Iran. Helminthologia 2016;53(3):285-9. [DOI:10.1515/helmin-2016-0017]
11. Mobedi I, Zare-Bidaki M, Siavashi MR, Naddaf SR, Kia EB, Mahmoudi M. Differential detection of Echinococcus spp. copro-DNA by nested-PCR in domestic and wild definitive hosts in Moghan Plain, Iran. Iran J Parasitol 2013;8(1):107. [PMCID]
12. Beiromvand M, Akhlaghi L, Fattahi Massom SH, Mobedi I, Meamar AR, Oormazdi H, et al. Detection of Echinococcus multilocularis in carnivores in Razavi Khorasan province, Iran using mitochondrial DNA. PLoS Negl Trop Dis 2011;5(11):e1379. [DOI:10.1371/journal.pntd.0001379] [PMID] [PMCID]
13. Malekifard F, Tavassoli M, Yakhchali M, Darvishzadeh R. Detection of Theileria equi and Babesia caballi using microscopic and molecular methods in horses in suburb of Urmia, Iran. Vet Res Forum 2014; 5(2):129. [PMCID]
14. Mathis A, Deplazes P, Eckert J. An improved test system for PCR-based specific detection of Echinococcus multilocularis eggs. J Helminthol 1996;70(03):219-22. [DOI:10.1017/S0022149X00015443] [PMID]
15. Eckert J, Gemmell MA, Meslin FX, Pawlowski ZS, World Health Organization. WHO/OIE manual on echinococcosis in humans and animals: a public health problem of global concern. World Organisation for Animal Health; 2001. [URL]
16. Nakao M, Yanagida T, Okamoto M, Knapp J, Nkouawa A, Sako Y, Ito A. State-of-the-art Echinococcus and Taenia: phylogenetic taxonomy of human-pathogenic tapeworms and its application to molecular diagnosis. Infect Genet Evol 2010;10(4):444-2. [DOI:10.1016/j.meegid.2010.01.011] [PMID]
17. Al-Sabi MN, Kapel CM. Multiplex PCR identification of Taenia spp. in rodents and carnivores. Parasitol Res 2011;109:1293-98. [DOI:10.1007/s00436-011-2373-9] [PMID]
18. Gholami I, Daryani A, Sharif M, Amouei A, Mobedi I. Seroepidemiological survey of helminthic parasites of stray dogs in Sari City, northern Iran. Pak J Biol Sci 2011;14(2):133-7. [DOI:10.3923/pjbs.2011.133.137] [PMID]
19. Shariatzadeh SA, Spotin A, Gholami S, Fallah E, Hazratian T, Mahami-Oskouei M et al. The first morphometric and phylogenetic perspective on molecular epidemiology of Echinococcus granulosus sensu lato in stray dogs in a hyperendemic Middle East focus, northwestern Iran. Parasite Vector 2015;8:1-0. [DOI:10.1186/s13071-015-1025-9] [PMID] [PMCID]
20. Eslami A, Ranjbar-Bahadori S, Meshgi B, Dehghan M, Bokaie S. Helminth infections of stray dogs from garmsar, Semnan province central Iran. Iran J Parasitol 2010;5(4):37. [PMCID]
21. Rokni MB. Echinococcosis/hydatidosis in Iran. Iran J Parasitol 2009;4(2):1-16. [Google Scholar]
22. Kohansal MH, Nourian A, Bafandeh S. Human cystic echinococcosis in Zanjan Area, Northwest Iran: a retrospective hospital based survey between 2007 and 2013 Iran J Public Health 2015;44(9):1277. [PMCID]
23. Torgerson PR, Budke CM. Echinococcosis-an international public health challenge. Res Vet Sci 2003;74(3):191-02. [DOI:10.1016/S0034-5288(03)00006-7] [PMID]
24. Borji H, Emami MR, Maleki MO, Razmi GH, Mehrjerdi HK, Moghaddas E. Alveolar echinococcosis infection in a monkey (Ateles geoffroyi) in Mashhad, Iran. Iran J Public Health 2012;41(2):111. [PMCID]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
