Volume 33, Issue 9 (December 2022)                   Studies in Medical Sciences 2022, 33(9): 676-683 | Back to browse issues page

Research code: 7109
Ethics code: IR.MAZUMS.REC.1399.420

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URL: http://umj.umsu.ac.ir/article-1-5918-en.html
Assistant professor, Department of biochemistry, Neyshabur branch, Islamic azad university, Neyshabur, Iran (corresponding author) , mehrzadjam@yahoo.com
Abstract:   (154 Views)
Background & Aims: Currently, cotoneaster is used as the herbal medicine to treat jaundice in neonates. The aim of this study was to create hyperbilirubinemia by phenylhydrazine and investigate the effects of cotoneaster on blood bilirubin levels in the rats with hyperbilirubinemia.
Materials & Methods: In the present experimental-interventional study, 70 µ/kg of phenylhydrazine was injected intraperitoneally to 15 days old Wistar rats. The rats with hyperbilirubinemia were divided to three groups. 48 hours later, cotoneaster with doses of 1, 2.5 and 5 mg/kg was fed to the animals through oropharyngeal tubes in 3 times for 10 days. Then, by taking blood and measuring total serum bilirubin at different hours, the curve of bilirubin changes was drawn up to 48 hours of the first injection of phenylhydrazine. Also, one group was selected as a control (healthy mice). 10 rats were selected in each group (5 females and 5 males). Descriptive statistics including mean and standard deviation were used for data analysis.
Results: The results showed the comparison of plasma bilirubin levels on day 0 and after phenylhydrazine injection increased significantly (up to 6 mg/dl) (p<0.05). The oral administration of cotoneaster in all three experimental doses caused a significant decrease in bilirubin in jaundiced rats compared to the control group, in such a way that the values were up to 0.2 mg/dl, up to 0.4 mg/dl and even up to 0 mg/dl reported (p < 0.05).
Conclusion: Animal study evidence showed the compound of phenylhydrazine has the ability to be used in creating an animal model of hyperbilirubinemia, and clay milk reduces its hyperbilirubinemic effects.
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Type of Study: Research | Subject: فارماکولوژی

1. Maisels MJ, McDonagh AF. Phototherapy for neonatal jaundice. N Engl J Med 2008;358(9):920-8. [DOI:10.1056/NEJMct0708376] [PMID]
2. Stevenson DK, Vreman HJ, Wong RJ, editors. Bilirubin production and the risk of bilirubin neurotoxicity. Seminars in perinatology; 2011: Elsevier. [DOI:10.1053/j.semperi.2011.02.005] [PMID]
3. Watchko J, Maisels M. Jaundice in low birthweight infants: pathobiology and outcome. Arch Dis Child 2003;88(6):F455-F8. [DOI:10.1136/fn.88.6.F455] [PMID] [PMCID]
4. Maamouri G, Boskabadi H, Mafinejad S, Bozorgnia Y, Khakshur A. Efficacy of oral zinc sulfate intake in prevention of neonatal jaundice. Iran J Neonat 2014;4(4):11-6. [URL]
5. Tarhani F, Momennasab M, Delfan B, Zendehkar A, Zaman M. A study of oral Taranjabin effects on reducing neonat's hyprebilirubinemia. Yafte 2004;6(3):55-9. [URL]
6. Dickoré WB, Kasperek G. Species of Cotoneaster (Rosaceae, Maloideae) indigenous to, naturalising or commonly cultivated in Central Europe. Willdenowia 2010;40(1):13-45. [DOI:10.3372/wi.40.40102]
7. Azadbakht M, Pishva N, Alinejad F. Effect of Manna from Cotoneaster discolor on infant Jaundice (effect on blood bilirubin level). J Med Plants 2005;4(14):36-44. [Google Scholar]
8. Fakhri M, Davoodi A, Hamzegardeshi Z, Farhadi R, Mousavinasab N, Keshtkar A, et al. Is cotoneaster manna improving the treatment of neonatal jaundice? Bangladesh J Pharmacol 2018;13(2):168-78. [DOI:10.3329/bjp.v13i2.36017]
9. Fakhri M, Farhadi R, Mousavinasab SN, Yosefi SS, Hosseinimehr SJ, Azadbakht M. Effect of natural products on jaundice in Iranian neonates. Jundishapur J Nat Pharmaceu Prod 2019;14 (1). [DOI:10.5812/jjnpp.83042]
10. Mansouri M, Ghotbi N, Bahadorbeigi L. Evaluation of the preventive effects of purgative manna on neonatal icterus in Sanandaj. Sci J Kurdistan Univ Med Sci 2012;17(2). [URL]
11. Ghotbi F. [Surveying the effect of cotoneaster spp. [shir khesht] on neonatal jaundice]. Res Med 2006;30(4):353-61. [URL]
12. Yin J, Wennberg RP, Miller M. Induction of hepatic bilirubin and drug metabolizing enzymes by individual herbs present in the traditional Chinese medicine, yin zhi huang. Dev Pharmacol Therapeut 1993;20:186-94. [DOI:10.1159/000457561] [PMID]
13. Saravanan R, Viswanathan P, Pugalendi KV. Protective effect of ursolic acid on ethanol-mediated experimental liver damage in rats. Life Sci 2006;78(7):713-8. [DOI:10.1016/j.lfs.2005.05.060] [PMID]
14. Memisoglu A, Kolgazi M, Yaman A, Bahadir E, Sirvanci S, Yeğen BÇ, et al. Neuroprotective effect of erythropoietin on phenylhydrazine-induced hemolytic hyperbilirubinemia in neonatal rats. Neurochem Res 2017;42:1026-37. [DOI:10.1007/s11064-016-2135-2] [PMID]
15. Ebbesen F. Determination of serum bilirubin concentration during phototherapy of newborns and in vitro: results compared by the direct spectrometric method and the diazo method. Clin Chem 1977;23(4):695-9. [DOI:10.1093/clinchem/23.4.695] [PMID]
16. Ameli Z, Assarroudi A, Akrami R. Effect of bilineaster drop on neonatal hyperbilirubinemia. Evid Based Care 2017;6(4):66-73. [URL]
17. Rafieian-Kopaei M, Khoshdel A, Kheiri S, Shemian R. Cotoneaster: A safe and easy way to reduce neonatal jaundice. Journal Clin Diagn Res 2016;10(4):SC01. [DOI:10.7860/JCDR/2016/17084.7574] [PMID] [PMCID]
18. Fakhri M, Davoodi A, Parviz M, Sadeghi Ghadi Z, Mousavinasab SN, Farhadi R, et al. Characterization and HPLC analysis of manna from some Cotoneaster species. Int J Pharm Sci Res 2017;8(12):5360-6. [Google Scholar]
19. Berger J. Phenylhydrazine haematotoxicity. J Appl Biomed 2007;5(3):125-30. [DOI:10.32725/jab.2007.017]
20. Shukla P, Yadav NK, Singh P, Bansode F, Singh R. Phenylhydrazine induced toxicity: a review on its haematotoxicity. Int J Basic Appl Med Sci 2012;2(2):86-91. [Google Scholar]
21. Gao Q, Zhao X, Yin L, Zhang Y, Wang B, Wu X, et al. The essential oil of Artemisia capillaris protects against CCl 4-induced liver injury in vivo. Revista Brasileira Farmacognosia 2016;26:369-74. [DOI:10.1016/j.bjp.2016.01.001]
22. Ridlon JM, Harris SC, Bhowmik S, Kang D-J, Hylemon PB. Consequences of bile salt biotransformations by intestinal bacteria. Gut Microbes 2016;7(1):22-39. [DOI:10.1080/19490976.2015.1127483] [PMID] [PMCID]
23. Bosek P, Nakano M. Hepatoprotective effect of rooibos tea (Aspalathus linearis) on CCl 4-induced liver damage in rats. Physiol Res 2003;52(4):461-6. [Google Scholar]
24. Monsef A, Eghbalian F, Rahimi N. Comparison of purgative manna drop and phototherapy with phototherapy treatment of neonatal jaundice: A randomized double-blind clinical trial. Osong Pub Health Res Persp 2019;10(3):152. [DOI:10.24171/j.phrp.2019.10.3.06] [PMID] [PMCID]
25. Farhat A, Mohammadzadeh A, Amir M, Ramezani M. Effect of cotoneaster tricolor pojark manna on serum bilirubin levels in neonates. Int J Pharmacol 2006;2(4):455-8. [DOI:10.3923/ijp.2006.455.458]

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