Volume 33, Issue 2 (May 2022)                   Studies in Medical Sciences 2022, 33(2): 116-130 | Back to browse issues page

Ethics code: IR.UMSU.REC.1399.068


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Jafarlu M, Zolfaghari M R, Fattahi A. THE EFFECT OF A PERIOD OF AEROBIC AND INTERVAL TRAINING ALONG WITH ASTAXANTHIN SUPPLEMENTATION ON THE EXPRESSION OF BAD, BAX, AND BCL2 GENES IN THE KIDNEY TISSUE OF RATS WITH TYPE 2 DIABETES. Studies in Medical Sciences 2022; 33 (2) :116-130
URL: http://umj.umsu.ac.ir/article-1-5826-en.html
PHD student of exercise physiology, Department of Exercise Physiology, Faculty of Sports Sciences, Urmia University, Urmia, Iran (Corresponding author) , mehdi.jafarlu@gmail.com
Abstract:   (1233 Views)
Background & Aims: Formation of free radicals in the kidney cells of diabetics leads to apoptosis of them and eventually nephropathy. The aim of this study was to investigate the effects of intervals and aerobic exercise along with astaxanthin supplementation on the expression levels of BAD, BAX and Bcl2 genes in the kidney tissue of rats with type 2 diabetes.
Materials & Methods: 35 male Wistar rats with an average weight of 197.46±15.55 grams were randomly divided into two non-diabetic (5 healthy controls) and diabetic (30) groups, and after induction of diabetes, they were randomly divided again in 6 groups (with 5 rats in each group) including diabetic control, diabetes+supplement, diabetes+interval, diabetes+supplement+interval, diabetes+aerobic, diabetes+aerobic+supplement. The exercise intervention groups performed the program of aerobic and interval exercises for eight weeks as five days per week. Supplement groups received 3 mg of astaxanthin supplement per kilogram of body weight dissolved in 0.3 ml of olive oil by gavage every day. The expression levels of BAD, BAX, and Bcl2 genes were measured in the kidney tissue of rats with type 2 diabetes. One-way analysis of variance and Tukey's post hoc test were used for data analysis.
Results: All exercise intervention protocols and astaxanthin supplementation had significant effects on the BAD index in the kidney tissue of the type 2 diabetic rats (p=0.001). All groups, except endurance training (p=0.890), had significant effects on BAX index in the kidney tissue of type 2 diabetic rats (p=0.001). Also, the use of astaxanthin supplement along with endurance training caused significant changes in Bcl2 index compared to single endurance training (p=0.001).
Conclusion: The intervention of aerobic and interval exercises with or without astaxanthin supplementation in the eight-week intervention period has positive effects on the apoptotic biomarkers in the kidney tissue of rats with type 2 diabetes.
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Type of Study: Clinical trials | Subject: Exercise physiology

References
1. Gonçalves N, Gomes-Ferreira C, Moura C, Roncon-Albuquerque Jr R, Leite-Moreira A, Falcão-Pires I. Worse cardiac remodeling in response to pressure overload in type 2 diabetes mellitus. Int J Cardiol 2016; 217:195-204. [DOI:10.1016/j.ijcard.2016.04.178] [PMID]
2. Rawal S, Manning P, Katare R. Cardiovascular microRNAs: as modulators and diagnostic biomarkers of diabetic heart disease. Cardiovasc. Diabetol 2014;13:44. [DOI:10.1186/1475-2840-13-44] [PMID] [PMCID]
3. Sheikh BA, Pari L, Rathinam A, Chandramohan R. Trans-anethole, a terpenoid ameliorates hyperglycemia by regulating key enzymes of carbohydrate metabolism in streptozotocin induced diabetic rats. Biochimie 2015; 112:57-65. [DOI:10.1016/j.biochi.2015.02.008] [PMID]
4. Li L, Yin Q, Tang X, Bai L, Zhang J, Gou S et al. C3a receptor antagonist ameliorates inflammatory and fibrotic signals in type 2 diabetic nephropathy by suppressing the activation of TGF-β/smad3 and IKBα pathway. PloS One 2014;9: e113639. [DOI:10.1371/journal.pone.0113639] [PMID] [PMCID]
5. Xu Y, Bai L, Chen X, Li Y, Qin Y, Meng X et al. 6-Shogaol ameliorates diabetic nephropathy through anti-inflammatory, hyperlipidemic, anti-oxidative activity in db/db mice. Biomedicine & Pharmacotherapy 2018; 97:633-41. [DOI:10.1016/j.biopha.2017.10.084] [PMID]
6. Narres M, Claessen H, Droste S, Kvitkina T, Koch M, Kuss O et al. The incidence of end-stage renal disease in the diabetic (compared to the non-diabetic) population: a systematic review. PloS One 2016;11:e0147329. [DOI:10.1371/journal.pone.0147329] [PMID] [PMCID]
7. Kashihara N, Haruna Y, K Kondeti V, S Kanwar Y. Oxidative stress in diabetic nephropathy. Curr Med Chem 2010;17:4256-69. [DOI:10.2174/092986710793348581] [PMID] [PMCID]
8. Pal PB, Sinha K, Sil PC. Mangiferin attenuates diabetic nephropathy by inhibiting oxidative stress mediated signaling cascade, TNFα related and mitochondrial dependent apoptotic pathways in streptozotocin-induced diabetic rats. PloS One 2014;9:e107220. [DOI:10.1371/journal.pone.0107220] [PMID] [PMCID]
9. Wagener F, Dekker D, Berden J, Scharstuhl A, Van der Vlag J. The role of reactive oxygen species in apoptosis of the diabetic kidney. Apoptosis 2009;14:1451-8. [DOI:10.1007/s10495-009-0359-1] [PMID] [PMCID]
10. Lee SH, Moon SJ, Paeng J, Kang H-Y, Nam BY, Kim S et al. Podocyte hypertrophy precedes apoptosis under experimental diabetic conditions. Apoptosis 2015; 20:1056-71. [DOI:10.1007/s10495-015-1134-0] [PMID]
11. Susztak K, Raff AC, Schiffer M, Böttinger EP. Glucose-induced reactive oxygen species cause apoptosis of podocytes and podocyte depletion at the onset of diabetic nephropathy. Diabetes 2006;55:225-33. [DOI:10.2337/diabetes.55.01.06.db05-0894] [PMID]
12. Sharifi A, Islami H, Larijani B. Investigating the role of the intrinsic pathway of apoptosis in cell death caused by high glucose concentration in PC12 cells. Iran. J Endocrinol Metab 2009; 9: 326-334. [URL]
13. Kim DY, Jung SY, Kim CJ, Sung YH, Kim JD. Treadmill exercise ameliorates apoptotic cell death in the retinas of diabetic rats. Mol Med Rep 2013;7:1745-50. [DOI:10.3892/mmr.2013.1439] [PMID]
14. Jafari A, Pourrazi H, Nikookheslat S, Baradaran BJ. Effect of exercise training on Bcl-2 and bax gene expression in the rat heart. Gene Cell Tissue 2015;2:1-10. (Persian) [DOI:10.17795/gct-32833]
15. Amini A, Gaeini A, Choobine S, Kordi MR, Alizadeh SJ. The Effects of Aerobic Training on Expression of Bcl-2 and miR-15 and Bcl-2 Protein in Tumor Tissue in Mice with Breast Cancer. Sport Physiol 2016; 8:85-100. (Persian). [Google Scholar]
16. Hussein MA. Cardioprotective effects of astaxanthin against isoproterenol-induced cardiotoxicity in rats. J Nutr Food Sci 2015; 5:1-6. [Google Scholar]
17. Al-Bulish MS, Xue C, Waly MI, Xu J, Wang Y, Tang Q. The Defensive Role of Antioxidants Astaxanthin against Oxidative Damage in Diabetic Rats Injected with Streptozotocin. J Food Nutr Res 2017; 5:191-6. [Google Scholar]
18. Lin X, Zhao Y, Li S. Astaxanthin attenuates glutamate-induced apoptosis via inhibition of calcium influx and endoplasmic reticulum stress. Eur J Pharmacol 2017;806:43-51. https://doi.org/10.1016/j.ejphar.2017.04.008 [DOI:10.1016/j.ejphar.2017.01.041] [PMID]
19. Fang Q, Guo S, Zhou H, Han R, Wu P, Han C. Astaxanthin protects against early burn-wound progression in rats by attenuating oxidative stress-induced inflammation and mitochondria-related apoptosis. Sci Rep 2017; 7:41440. [DOI:10.1038/srep41440] [PMID] [PMCID]
20. Guo SX, Zhou HL, Huang CL, You CG, Fang Q, Wu P et al. Astaxanthin attenuates early acute kidney injury following severe burns in rats by ameliorating oxidative stress and mitochondrial-related apoptosis. Mar Drugs 2015; 13:2105-23. [DOI:10.3390/md13042105] [PMID] [PMCID]
21. Manabe E, Handa O, Naito Y, Mizushima K, Akagiri S, Adachi S et al. Astaxanthin protects mesangial cells from hyperglycemia‐induced oxidative signaling. J Cell Biochem 2008;103:1925-37. [DOI:10.1002/jcb.21583] [PMID]
22. Naito Y, Uchiyama K, Aoi W, Hasegawa G, Nakamura N, Yoshida N et al. Prevention of diabetic nephropathy by treatment with astaxanthin in diabetic db/db mice. Biofactors 2004; 20:49-59. [DOI:10.1002/biof.5520200105] [PMID]
23. Sila A, Ghlissi Z, Kamoun Z, Makni M, Nasri M, Bougatef A et al. Astaxanthin from shrimp by-products ameliorates nephropathy in diabetic rats. Eur J Nutr 2015; 54:301-7. [DOI:10.1007/s00394-014-0711-2] [PMID]
24. Boor P, Celec P, Behuliak M, Grančič P, Kebis A, Kukan M et al. Regular moderate exercise reduces advanced glycation and ameliorates early diabetic nephropathy in obese Zucker rats. Metabolism 2009;58:1669-77. [DOI:10.1016/j.metabol.2009.05.025] [PMID]
25. Viberti G, Jarrett R, McCartney M, Keen H. Increased glomerular permeability to albumin induced by exercise in diabetic subjects. Diabetologia 1978;14:293-300. [DOI:10.1007/BF01223019] [PMID]
26. Kurdak H, Sandikci S, Ergen N, Dogan A, Kurdak SS. The effects of regular aerobic exercise on renal functions in streptozotocin induced diabetic rats. J Sports Sci Med 2010;9:294. [PMCID]
27. Podhorska-Okołów M, Dziegiel P, Gomułkiewicz A, Dolińska-Krajewska B, Murawska-Ciałowicz E, Jethon Z et al. The role of AT1 and AT2 angiotensin receptors in the mechanism of apoptosis in renal tubular cells after physical exercise. Rocz Akad Med Bialymst 2004; 49:8-10. [Google Scholar]
28. Podhorska-Okołów M, Dzięgiel P, Murawska-Ciałowicz E, Krajewska B, Ciesielska U, Jethon Z et al. Exercise-induced apoptosis in renal tubular cells of the rat. Folia Morphologica 2004; 63:213-6. [PMID]
29. Ngarestani HR, Hosseinpour-Delavar S, Azizi M, Azarbaijani MA, Farzangi P. The effect of combination of regular continuous exercise and resveratrol supplementation on some regulatory and executive factors of hepatocytic apoptosis in male diabetic rats. Kashan Univ Med Sci J 2020;23:605-14. [Google Scholar]
30. Chen KC, Peng CC, Hsieh CL, Peng RY. Exercise ameliorates renal cell apoptosis in chronic kidney disease by intervening in the intrinsic and the extrinsic apoptotic pathways in a rat model. Evid Based Complement Alternat Med 2013; 20: 368450. [DOI:10.1155/2013/368450] [PMID] [PMCID]
31. Ataei G, Rahbarian R. Investigating the effect of safranal on Bax and Bcl2 and oxidative stress levels in testis tissue of streptozotocin-induced diabetic rats. Kashan Univ Med Sci J 2020;24:10-20. [Google Scholar]
32. Farzanegi P, Habibian M, Alinejad H. The combined effect of regular aerobic exercise with garlic extracts on renal apoptosis regulatory factors in aged rats with chronic kidney disease. Arak Med Univ J 2016; 19:62-70. (Persian). [Google Scholar]
33. Delfan M, Rabiee M, Amadeh Juybari R. Synergistic Effect of High Intensity Interval Training (Hiit) Combined with Curcumin on Bax and Bcl-2 Gene Expression in Soleus Muscle of Diabetic Rats. Iran J Diabetes Metabol 2021 10; 20:210-9. (Persian). [Google Scholar]
34. Jabbari SE, Gholami M, Nikbakht H, Shakeri N, Ghazalian F. Effect of aerobic training and L-carnitine supplementation on some apoptotic factors in diabetic rat liver. Razi J Med Sci 2019; 26:131-140. (Persian). [DOI:10.18502/ijdo.v12i1.3635]
35. Cai MX, Shi XC, Chen T, Tan ZN, Lin QQ, Du SJ et al. Exercise training activates neuregulin 1/ErbB signaling and promotes cardiac repair in a rat myocardial infarction model. Life Sci 2016; 149:1-9. [DOI:10.1016/j.lfs.2016.02.055] [PMID]
36. Aboutaleb N, Shamsaei N, Khaksari M, Erfani S, Rajabi H, Nikbakht F et al. pre-ischemic exercise reduces apoptosis in hippocampal CA3 cells after cerebral ischemia by modulation of the Bax/Bcl-2 proteins ratio and prevention of caspase-3 activation. J Physiol Sci 2015; 65:435-43. (Persian) [DOI:10.1007/s12576-015-0382-7] [PMID]
37. Schlondorff DO. Overview of factors contributing to the pathophysiology of progressive renal disease. Kidney Int 2008;74:860-6. [DOI:10.1038/ki.2008.351] [PMID]
38. Frances DE, Ronco MT, Monti JA, Ingaramo PI, Pisani GB, Parody JP et al. Hyperglycemia induces apoptosis in rat liver through the increase of hydroxyl radical: new insights into the insulin effect. J Endocrinol 2010; 205: 187-200. [DOI:10.1677/JOE-09-0462] [PMID]
39. Reed JC. Bcl-2 and the regulation of programmed cell death. J Cell Biol 1994; 124:1-6. [DOI:10.1083/jcb.124.1.1] [PMID] [PMCID]
40. Alamdari N, Aversa Z, Castillero E, Gurav A, Petkova V, Tizio S et al. Resveratrol prevents dexamethasone-induced expression of the muscle atrophy-related ubiquitin ligases atrogin-1 and MuRF1 in cultured myotubes through a SIRT1- dependent mechanism. Biochem Biophys Res Commun 2012; 417: 528-33. (Persian). [DOI:10.1016/j.bbrc.2011.11.154] [PMID] [PMCID]
41. Yang B, Johnson TS, Thomas GL, Watson PF, Wagner B, Furness PN et al. A shift in the Bax/Bcl-2 balance may activate caspase-3 and modulate apoptosis in experimental glomerulonephritis. Kidney Int 2002; 62: 1301-13. [DOI:10.1111/j.1523-1755.2002.kid587.x] [PMID]
42. Pollack M, Phaneuf S, Dirks A, Leeuwenburgh C. The role of apoptosis in the normal aging brain, skeletal muscle, and heart. Ann N Y Acad Sci 2002; 959: 93-107. [DOI:10.1111/j.1749-6632.2002.tb02086.x] [PMID]
43. Kwak H-B, Lee Y, Kim JH, Van Remmen H, Richardson AG, Lawler JM, et al. MnSOD overexpression reduces fibrosis and pro-apoptotic signaling in the aging mouse heart. Biol Sci Med Sci 2015;70: 533-44. [DOI:10.1093/gerona/glu090] [PMID] [PMCID]
44. Dela F, Ingersen A, Andersen NB, Nielsen MB, Petersen HH, Hansen CN et al. Effects of one‐legged high‐intensity interval training on insulin‐mediated skeletal muscle glucose homeostasis in patients with type 2 diabetes. Acta Physiologica 2019; 226: e13245. [DOI:10.1111/apha.13245] [PMID]
45. Hong JH, Kim MJ, Park MR, Kwag OG, Lee IS, Byun BH et al. Effects of vitamin E on oxidative stress and membrane fluidity in brain of streptozotocin induced diabetic Rats. Clin Chim Acta 2004; 340:107-15. [DOI:10.1016/j.cccn.2003.10.003] [PMID]
46. Chen K-C, Peng C-C, Hsieh C-L, Peng RY. Exercise ameliorates renal cell apoptosis in chronic kidney disease by intervening in the intrinsic and the extrinsic apoptotic pathways in a rat model. Evid. based Complement. Altern Med 2013;1:1-8. [DOI:10.1155/2013/368450] [PMID] [PMCID]
47. Almasi S, Shahsavandi B, Aliparasty MR, Alipour MR, Rahnama B, Feizi H. The antiapoptotic effect of ghrelin in the renal tissue of chronic hypoxic rats. J Physiol Pharmacol 2015; 19:114-20. [Google Scholar]
48. Zhang Y, Yao HP, Huang FF, Wu W, Gao Y, Chen ZB et al. Allicin, a major component of garlic, inhibits apoptosis in vital organs in rats with trauma/hemorrhagic shock. Crit Care Med 2008;36:3226-32. [DOI:10.1097/CCM.0b013e31818f2103] [PMID]
49. Polyakov NE, Leshina TV, Konovalova TA, Kispert LD. Carotenoids as scavengers of free radicals in a Fenton reaction: antioxidants or pro-oxidants? Free Radic Biol Med 2001;31:398-404. [DOI:10.1016/S0891-5849(01)00598-6] [PMID]
50. Focsan AL, Pan S, Kispert LD. Electrochemical study of astaxanthin and astaxanthin n-octanoic monoester and diester: Tendency to form radicals. J Phys Chem 2014;118:2331-9. [DOI:10.1021/jp4121436] [PMID]
51. Yang M, Zhao T, Deng T, Wang Z. Protective effects of astaxanthin against diabetic retinal vessels and pro-inflammatory cytokine synthesis. Int J Clin Exp Med 2019;12:4725-34. [Google Scholar]
52. Cui G, Li L, Xu W, Wang M, Jiao D, Yao B et al. Astaxanthin protects ochratoxin A-induced oxidative stress and apoptosis in the heart via the Nrf2 pathway. Oxid Med Cell 2020; 22:1-6. https://doi.org/10.1155/2020/7639109 https://doi.org/10.1155/2020/8638301 https://doi.org/10.1155/2020/7948705 https://doi.org/10.1155/2020/8829328 [DOI:10.1155/2020/9253745] [PMID] [PMCID]
53. Azizzadeh T, Zolfaghari MR, Fattahi A, Khademvatani K. Effect of moderate intensity continuos training with astaxantin supplementation on inflammatory factors of cardiac tissue in type 2 diabetic rats. Ebnesina 2021; 23:12-22. (Persian) [Google Scholar]
54. Said M, Al-Bulish M, Xue C, Waly M, Xu J, Wang YM et al. The defensive role of antioxidants astaxanthin against oxidative damage in diabetic rats injected with streptozotocin. J. Food Nutr Res 2017; 5:191-196. [Google Scholar]
55. Hajhashemy Z, Saneei P. Meta-analysis of astaxanthin supplementation on obesity, blood pressure, CRP, glycemic biomarkers, and lipid profile: reanalysis is needed. Pharmacol Res 2020:12:105171 [DOI:10.1016/j.phrs.2020.105171] [PMID]

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