Volume 33, Issue 5 (August 2022)                   Studies in Medical Sciences 2022, 33(5): 322-335 | Back to browse issues page

Ethics code: این مطالعه برگرفته از رساله دکترای تخصصی فیزیولوژی ورز


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Bahram M E, Afroundeh R, Pourvaghar M J, Seyfi F, Katebi L. STUDYING THE SIMULTANEOUS EFFECT OF COMBINED EXERCISES AND SUPPLEMENTATION OF MULBERRY LEAF EXTRACT ON SOME INFLAMMATORY BIOMARKERS EFFECTIVE IN THE PATHOGENESIS OF DIABETES AND HBA1C IN ELDERLY MEN WITH TYPE 2 DIABETES. Studies in Medical Sciences 2022; 33 (5) :322-335
URL: http://umj.umsu.ac.ir/article-1-5824-en.html
Associate Professor of Sports Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran , afroundeh@uma.ac.ir
Abstract:   (1113 Views)
Background and Aims: Adipokines play an important role in inflammation and pathogenesis of diabetes. The aim of this study was to study the simultaneous effect of combined exercises (aerobic + resistance) and the consumption of mulberry leaf extract on the serum levels of galectin-3, apelin, and HbA1C in elderly men with type 2 diabetes
Materials and Methods: In this semi-experimental and single-blind research, 40 elderly men with the age range of 67.07 ± 7.48 years were purposefully selected and randomly divided into five equal groups (exercise, supplement, Supplement+exercise, placebo, and control). A daily dose of 1000 mg (two 500 mg capsules) of Mulberry leaf extract was used 3 times a day for 2 months. The exercise and exercise + supplement groups practiced for 8 weeks in 3 sessions, each week for 90 minutes. The studied variables were measured 24 hours before and 48 hours after the intervention period.
Results: Galectin-3, apelin, and HbA1C levels in the post-test of the exercise, exercise+supplement, and supplement groups were significantly reduced compared to the pre-test (P<0.05). Also, the results of covariance analysis showed that the levels of galectin-3, apelin, and HbA1C in the exercise, exercise+supplement and supplement groups were significantly reduced compared to the placebo and control groups (P<0.05).
Conclusion: It seems that combined exercises and consumption of Mulberry leaf extract can be effective in reducing inflammatory biomarkers effective in the pathogenesis of diabetes in the elderly with type 2 diabetes. However, supplementing with combined exercise has the greatest effect on controlling these inflammatory markers.
Full-Text [PDF 611 kb]   (700 Downloads)    
Type of Study: Research | Subject: Exercise physiology

References
1. Stewart K. Exercise training: can it improve cardiovascular health in patients with type 2 diabetes? Br J Sports Med 2004;38(3):250-2. [DOI:10.1136/bjsm.2004.012187] [PMID] [PMCID]
2. Al-Saeed AH, Constantino MI, Molyneaux L, D'Souza M, Limacher-Gisler F, Luo C, et al. An inverse relationship between age of type 2 diabetes onset and complication risk and mortality: the impact of youth-onset type 2 diabetes. Diabetes Care 2016;39(5):823-9. [DOI:10.2337/dc15-0991] [PMID]
3. Yamauchi T, Iwabu M, Okada-Iwabu M, Kadowaki T. Adiponectin receptors: a review of their structure, function and how they work. Best Pract Res Clin Endocrinol Metab 2014;28(1):15-23. [DOI:10.1016/j.beem.2013.09.003] [PMID]
4. Filipe MD, Meijers WC, van der Velde AR, de Boer RA. Galectin-3 and heart failure: prognosis, prediction & clinical utility. Clin Chim Acta 2015;443:48-56 [DOI:10.1016/j.cca.2014.10.009] [PMID]
5. Menini S, Iacobini C, Blasetti Fantauzzi C, Pesce CM, Pugliese G. Role of galectin-3 in obesity and impaired glucose homeostasis. Oxid Med Cell Longev 2016;2016. [DOI:10.1155/2016/9618092] [PMID] [PMCID]
6. Sriram DK, Gowri G, Haridoss S, Venkatesan S, Rajendran I, George M. Association of galectin-3 with high cardiovascular risk in patients with type 2 diabetes attending a multi-specialty hospital in South India. J Res Med Sci 2016;4(12):5417-21. [DOI:10.18203/2320-6012.ijrms20164220]
7. Vora A, De Lemos JA, Ayers C, Grodin JL, Lingvay I. Association of galectin-3 with diabetes mellitus in the dallas heart study. J Clin Endocrinol Metab 2019;104(10):4449-58. [DOI:10.1210/jc.2019-00398] [PMID]
8. Abd El-Kader SM, Al-Jiffri OH, Al-Shreef FM. Aerobic exercises alleviate symptoms of fatigue related to inflammatory cytokines in obese patients with type 2 diabetes. Afr Health Sci 2015;15(4):1142-8. [DOI:10.4314/ahs.v15i4.13] [PMID] [PMCID]
9. Moghadami K, Khalafi M, Shabani M. The effect of aerobic training on serum levels of Galectin3 and inflammatory markers in elderly women with metabolic syndrome: a randomized clinical trial. Jundishapur Sci Med J 2020;18(6):639-48. [URL]
10. Siuf Jahromi M, Ebrahim K, Babaeibeigi M, Nikbakht H. Effect of Cardiac Rehabilitation with Severe Periodic Exercise Activity on Serum Glycine3 and Mutagenic Fraction in Patients with Heart Failure. Armaghan Danesh 2018;22(6):754-65. [Google Scholar]
11. Li Y, Li T, Zhou Z, Xiao Y. Emerging roles of Galectin-3 in diabetes and diabetes complications: A snapshot. Rev Endocr Metab Disord 2022:1-9. [DOI:10.1007/s11154-021-09704-7] [PMID] [PMCID]
12. Bełtowski J. Apelin and visfatin: unique" beneficial" adipokines upregulated in obesity? Med Sci Monit 2006;12(6):RA112-9. [Google Scholar]
13. Soriguer F, Garrido-Sanchez L, Garcia-Serrano S, Garcia-Almeida JM, Garcia-Arnes J, Tinahones FJ, et al. Apelin levels are increased in morbidly obese subjects with type 2 diabetes mellitus. Obes Surg 2009;19(11):1574-80. [DOI:10.1007/s11695-009-9955-y] [PMID]
14. Mughal A, O'Rourke ST. Vascular effects of apelin: Mechanisms and therapeutic potential. Pharmacol Ther 2018;190:139-47. [DOI:10.1016/j.pharmthera.2018.05.013] [PMID] [PMCID]
15. Kazemi A, Rahmati M, Akhondi M. Effect of 6 Weeks of High-Intensity Interval Training with Cinnamon Supplementation on Serum Apelin Concentration and Insulin Resistance in Overweight Boys. Horiz Med Sci 2016;22(3):177-83. [DOI:10.18869/acadpub.hms.22.3.177]
16. Shehata MI, Ahmed SA, Gomaa RS, Abulmeaty MM. Effect of apelin on insulin resistance, beta cell function and lipid profile in healthy and diabetic rat models. Zagazig Univ Med J 2015;19(1). [Google Scholar]
17. Mohebbi H, Rahmani Nia F, Hedayati Emami M, Saeidi Ziabari T. The effect of 8 weeks moderate intensity aerobic exercise on plasma Apelin level and insulin resistance in women with type 2 diabetes. Sports Physiol 2014;5(20):115-28. [Google Scholar]
18. Kadoglou NP, Vrabas IS, Kapelouzou A, Lampropoulos S, Sailer N, Kostakis A, et al. The impact of aerobic exercise training on novel adipokines, apelin and ghrelin, in patients with type 2 diabetes. Med Sci Monit 2012;18(5):CR290. [DOI:10.12659/MSM.882734] [PMID] [PMCID]
19. Afshounpour MT, Habibi A, Ranjbar R. Impact of combined exercise training on plasma concentration of Apelin, resistin and insulin resistance in patients with type 2 diabetics' male. Hormozgan Med J 2016;20(3). [Google Scholar]
20. Pinent M, Cedo L, Montagut G, Blay M, Ardévol A. Procyanidins improve some disrupted glucose homoeostatic situations: an analysis of doses and treatments according to different animal models. Crit Rev Food Sci Nutr 2012;52(7):569-84. [DOI:10.1080/10408398.2010.501533] [PMID]
21. Işık H, Çevikbaş A, Gürer ÜS, Kıran B, Üresin Y, Rayaman P, et al. Potential adjuvant effects of Nigella sativa seeds to improve specific immunotherapy in allergic rhinitis patients. Med Princ Pract 2010;19(3):206-11. [DOI:10.1159/000285289] [PMID]
22. Kim JY, Chung HI, Jung K-O, Wee J-H, Kwon O. Chemical profiles and hypoglycemic activities of mulberry leaf extracts vary with ethanol concentration. Food Sci Biotechnol 2013;22(5):1-5. https://doi.org/10.1007/s10068-013-0235-1 https://doi.org/10.1007/s10068-013-0233-3 https://doi.org/10.1007/s10068-013-0205-7 https://doi.org/10.1007/s10068-013-0218-2 https://doi.org/10.1007/s10068-013-0228-0 [DOI:10.1007/s10068-013-0211-9]
23. Kimura T, Nakagawa K, Kubota H, Kojima Y, Goto Y, Yamagishi K, et al. Food-grade mulberry powder enriched with 1-deoxynojirimycin suppresses the elevation of postprandial blood glucose in humans. J Agric Food Chem 2007;55(14):5869-74. [DOI:10.1021/jf062680g] [PMID]
24. Tian S, Wang M, Liu C, Zhao H, Zhao B. Mulberry leaf reduces inflammation and insulin resistance in type 2 diabetic mice by TLRs and insulin Signalling pathway. BMC Complement Altern Med 2019;19(1):1-12. [DOI:10.1186/s12906-019-2742-y] [PMID] [PMCID]
25. Farzanegi P, Samiee M, Sabbaghian Z. Interactive effect of regular aerobic training and milk consumption on some inflammatory markers and lipid profile in overweight boys. Koomesh 2016;17(3). [Google Scholar]
26. Sobhani F, Haghshenas R, Rahimi M. Effect of eight weeks aerobic training and supplementation of green tea on apelin plasma levels and insulin resistance in elderly women with type 2 diabetes. J. Maz Univ Med 2019;28(170):84-93. [Google Scholar]
27. Tofighi A, Babaei S. The effects of separate and combined exercise and Nigella supplement on plasmatic levels of apelin and glucose in type 2 diabetes mouse. Stud Med Sci 2016;27(1):10-8. [Google Scholar]
28. Touvra A-M, Volaklis KA, Spassis AT, Zois CE, Douda HT, Kotsa K, et al. Combined strength and aerobic training increases transforming growth factor-β1 in patients with type 2 diabetes. Hormones 2011;10(2):125-30. [DOI:10.14310/horm.2002.1302] [PMID]
29. Shenoy S, Guglani R, Sandhu JS. Effectiveness of an aerobic walking program using heart rate monitor and pedometer on the parameters of diabetes control in Asian Indians with type 2 diabetes. Prim Care Diabetes 2010;4(1):41-5. [DOI:10.1016/j.pcd.2009.10.004] [PMID]
30. Seyedizadeh SH, Cheragh-Birjandi S, Hamedi Nia MR. The effects of combined exercise training (resistance-aerobic) on serum kinesin and physical function in type 2 diabetes patients with diabetic peripheral neuropathy (randomized controlled trials). J Diabetes Res 2020;2020. [DOI:10.1155/2020/6978128] [PMID] [PMCID]
31. Riche DM, Riche KD, East HE, Barrett EK, May WL. Impact of mulberry leaf extract on type 2 diabetes (Mul-DM): a randomized, placebo-controlled pilot study. Complement Ther Med 2017;32:105-8. [DOI:10.1016/j.ctim.2017.04.006] [PMID]
32. Aliniya N, Elmieh A, Fadaei Chafy MR. Interaction effect of combined exercise and supplementation with portulaca oleracea on liver enzymes in obese postmenopausal women with non-alcoholic fatty liver disease. Complement Med J 2020;10(1):68-79. [DOI:10.32598/cmja.10.1.960.1]
33. Ghaffarpour M, Houshiar A, Kianfar H. Household of Scales Guide, Conversion coefficients and Percent of Edible food. Tehran, Publication of Agricultural Sciences 2000;25:24-9. [URL]
34. Jackson AS, Pollock ML. Generalized equations for predicting body density of men. Br J Nutr 1978;40(3):497-504. [DOI:10.1079/BJN19780152] [PMID]
35. Khajeian N, Moghadasi M. Effect of 8 weeks regular endurance training on galectin-3 changes after a strenuous aerobic exercise. J Phys Act Hormon 2017;1(3):29-38. [Google Scholar]
36. Billebeau G, Vodovar N, Sadoune M, Launay J-M, Beauvais F, Cohen-Solal A. Effects of a cardiac rehabilitation programme on plasma cardiac biomarkers in patients with chronic heart failure. Eur J Prev Cardiol 2017;24(11):1127-35. [DOI:10.1177/2047487317705488] [PMID]
37. Pang J, Rhodes DH, Pini M, Akasheh RT, Castellanos KJ, Cabay RJ, et al. Increased adiposity, dysregulated glucose metabolism and systemic inflammation in Galectin-3 KO mice. PLoS One 2013;8(2):e57915. [DOI:10.1371/journal.pone.0057915] [PMID] [PMCID]
38. Pejnovic NN, Pantic JM, Jovanovic IP, Radosavljevic GD, Milovanovic MZ, Nikolic IG, et al. Galectin-3 deficiency accelerates high-fat diet-induced obesity and amplifies inflammation in adipose tissue and pancreatic islets. Diabetes 2013;62(6):1932-44. [DOI:10.2337/db12-0222] [PMID] [PMCID]
39. Nayor M, Wang N, Larson MG, Vasan RS, Levy D, Ho JE. Circulating galectin‐3 is associated with cardiometabolic disease in the community. J Am Heart Assoc 2015;5(1):e002347. [DOI:10.1161/JAHA.115.002347] [PMID] [PMCID]
40. Song X, Qian X, Shen M, Jiang R, Wagner MB, Ding G, et al. Protein kinase C promotes cardiac fibrosis and heart failure by modulating galectin-3 expression. Biochim Biophys Acta Mol Cell Res 2021 ;1853(2):513-21. [DOI:10.1016/j.bbamcr.2014.12.001] [PMID]
41. Hashemi Z, Moghadasi M. Comparison of Galectin-3 and NT-ProBNP levels in strength and endurance elite athletes. Metabol Exerc 2017;6(1):49-57. [Google Scholar]
42. Fernandes-Silva MM, Guimaraes GV, Rigaud VO, Lofrano-Alves MS, Castro RE, de Barros Cruz LG, et al. Inflammatory biomarkers and effect of exercise on functional capacity in patients with heart failure: Insights from a randomized clinical trial. Eur J Prev Cardiol 2017;24(8):808-17. [DOI:10.1177/2047487317690458] [PMID]
43. Hughes R. The galectin family of mammalian carbohydrate-binding molecules. Biochem Soc Trans 1997;25(4):1194-8. [DOI:10.1042/bst0251194] [PMID]
44. Hättasch R, Spethmann S, de Boer RA, Ruifrok WP, Schattke S, Wagner M, et al. Galectin-3 increase in endurance athletes. Eur J Prev Cardiol 2014;21(10):1192-9. [DOI:10.1177/2047487313492069] [PMID]
45. Gao Z, Liu Z, Wang R, Zheng Y, Li H, Yang L. Galectin-3 is a potential mediator for atherosclerosis. J Immunol Res 2020;2020. [DOI:10.1155/2020/5284728] [PMID] [PMCID]
46. Zhu S, Sun F, Li W, Cao Y, Wang C, Wang Y, et al. Apelin stimulates glucose uptake through the PI3K/Akt pathway and improves insulin resistance in 3T3-L1 adipocytes. Mol Cell Biochem 2011;353(1):305-13. [DOI:10.1007/s11010-011-0799-0] [PMID]
47. Trayhurn P, Wood IS. Adipokines: inflammation and the pleiotropic role of white adipose tissue. Br J Nutr 2004;92(3):347-55. [DOI:10.1079/BJN20041213] [PMID]
48. Gielen S, Adams V, Möbius-Winkler S, Linke A, Erbs S, Yu J, et al. Anti-inflammatory effects of exercise training in the skeletal muscle of patients with chronic heart failure. J Am Coll Cardiol 2003;42(5):861-8. [DOI:10.1016/S0735-1097(03)00848-9] [PMID]
49. Chaweewannakorn C, Nyasha MR, Chen W, Sekiai S, Tsuchiya M, Hagiwara Y, et al. Exercise‐evoked intramuscular neutrophil‐endothelial interactions support muscle performance and GLUT4 translocation: a mouse gnawing model study. J Physiol 2020;598(1):101-22. [DOI:10.1113/JP278564] [PMID]
50. Holten MK, Zacho M, Gaster M, Juel C, Wojtaszewski JF, Dela F. Strength training increases insulin-mediated glucose uptake, GLUT4 content, and insulin signaling in skeletal muscle in patients with type 2 diabetes. Diabetes 2004;53(2):294-305. [DOI:10.2337/diabetes.53.2.294] [PMID]
51. Dray C, Sakar Y, Vinel C, Daviaud D, Masri B, Garrigues L, et al. The intestinal glucose-apelin cycle controls carbohydrate absorption in mice. Gastroenterology 2013;144(4):771-80. [DOI:10.1053/j.gastro.2013.01.004] [PMID]
52. Attané C, Foussal C, Le Gonidec S, Benani A, Daviaud D, Wanecq E, et al. Apelin treatment increases complete Fatty Acid oxidation, mitochondrial oxidative capacity, and biogenesis in muscle of insulin-resistant mice. Diabetes 2012;61(2):310-20. [DOI:10.2337/db11-0100] [PMID] [PMCID]
53. Jafari M, Yekrangi Z, Marhamati M, Reyhani M, Karimi H. Acute effects of aerobic and anaerobic activities on blood adipokines and vascular adhesive molecules in young women. J Appl Health Stud Sport Physiol 2020;7(1):65-72. [Google Scholar]
54. Afshounpour MT, Habibi A, Ranjbar R. Impact of combined exercise training on plasma concentration of Apelin, resistin and insulin resistance in patients with type 2 diabetics' male. Hormozgan Med J 2016;20(3). [Google Scholar]
55. Atashak S, Niloofari A, Azizbaygi K. The effect of blackberry extract on the total antioxidant capacity and lipid peroxidation after acute resistance exercise in obese men. J Food Technol Nutr 2014;11(2):55-62. [Google Scholar]
56. Rani P, Unni KM, Karthikeyan J. Evaluation of antioxidant properties of berries. Indian J Clin Biochem 2004;19(2):103-10. [DOI:10.1007/BF02894266] [PMID] [PMCID]
57. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 1999;64(4):555-9. [DOI:10.1016/S0308-8146(98)00102-2]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Studies in Medical Sciences

Designed & Developed by : Yektaweb