Volume 33, Issue 8 (November 2022)
Studies in Medical Sciences 2022, 33(8): 610-620 |
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Mehdizadeh M, Ghiasi M, Bagheri N. USE OF PRODUCTS EXTRACTED FROM BLOOD TO DESIGN BIOACTIVE STRUCTURES IN REGENERATIVE MEDICINE. Studies in Medical Sciences 2022; 33 (8) :610-620
URL: http://umj.umsu.ac.ir/article-1-5793-en.html
URL: http://umj.umsu.ac.ir/article-1-5793-en.html
Researcher in Iranian Tissue Bank & Research Center, Tehran University of Medical Sciences, Tehran, Iran (Corresponding Author) , mahdieh.ghiasi@yahoo.com
Abstract: (1951 Views)
Background & Aim: Nowadays, according to the advances in medical science, blood products are considered as one of the important biological tools. In this regard, the use of blood-derived biomaterials in reconstructive medicine to repair a variety of tissues has expanded. One of these products is based on platelet separation, which is obtained by different processing techniques and is used alone or in combination with a variety of synthetic or natural structures. These platelet products contain a large number of growth factors that play key roles in the process of tissue repair and regeneration. The purpose of this study was to investigate the importance of these products in the repair of different body tissues.
Materials & Methods: This study is a review one that was conducted by electronic search in PubMed and EMBASE databases from 2000 to 2021 with keywords such as platelet⸲ growth factors⸲ stem cells and regenerative medicine.
Results: Findings from studies show that blood-derived biomaterials can be extracted by different techniques, and these products have different types of growth factors such as VEGF, PDGF, EGF, and TGF-β, which can contribute to faster tissue regeneration alone or in combination with other substances.
Conclusion: Studies show that blood-derived platelet products can facilitate and accelerate the healing process of various tissues. They can also help advance therapeutic goals.
Materials & Methods: This study is a review one that was conducted by electronic search in PubMed and EMBASE databases from 2000 to 2021 with keywords such as platelet⸲ growth factors⸲ stem cells and regenerative medicine.
Results: Findings from studies show that blood-derived biomaterials can be extracted by different techniques, and these products have different types of growth factors such as VEGF, PDGF, EGF, and TGF-β, which can contribute to faster tissue regeneration alone or in combination with other substances.
Conclusion: Studies show that blood-derived platelet products can facilitate and accelerate the healing process of various tissues. They can also help advance therapeutic goals.
Type of Study: Review article |
Subject:
فارماکولوژی
References
1. Suárez-Barrio C, Etxebarria J, Hernáez-Moya R, Val-Alonso M, Rodriguez-Astigarraga M, Urkaregi A,Freire V, Morales M.C, Durán J.A, Vicario M et al. Hyaluronic Acid Combined with Serum Rich in Growth Factors in Corneal Epithelial Defects. Int J Mol Sci 2019;20:1655. [DOI:10.3390/ijms20071655] [PMID] [PMCID]
2. Ghiasi, M, Tabatabaei Qomi R, Kalhor N, Mehdizadeh M, Sheykhhasan M. Survival Potential Investigation of the Adipose-Derived Mesenchymal Stem Cell in the Natural Scaffolds as a Suitable Growth Medium. J Cell Tissue 2015;6(1):23-9. Persian. [Google Scholar]
3. Panda S, Karanxha L, Goker F, Satpathy A, Taschieri S et al. Autologous Platelet Concentrates in Treatment of Furcation Defects-A Systematic Review and Meta-Analysis. Int J Mol Sci 2019;20:1347. [DOI:10.3390/ijms20061347] [PMID] [PMCID]
4. KawaseT, Nagata M, Okuda K, Ushiki T, Fujimoto Y, Watanabe M et al. Platelet-Rich Fibrin Extract: A Promising Fetal Bovine Serum Alternative in Explant Cultures of Human Periosteal Sheets for Regenerative Therapy. Int J Mol Sci 2019;20:1053. [DOI:10.3390/ijms20051053] [PMID] [PMCID]
5. Kaminski R, Maksymowicz-Wleklik M, Kulinski K, Kozar-Kaminska K, Dabrowska-Thing A, Pomianowski S. Short-Term Outcomes of Percutaneous Trephination with a Platelet Rich Plasma Intrameniscal Injection for the Repair of Degenerative Meniscal Lesions. A Prospective, Randomized, Double-Blind, Parallel-Group, Placebo-Controlled Study. Int J Mol Sci 2019;20:856. [DOI:10.3390/ijms20040856] [PMID] [PMCID]
6. Ghiasi M, Farzaneh S, Bigdelo M. Assessment of human cartilage regeneration in a patient with knee osteoarthritis using autologous adipose-tissue- derived stem cells and Platelet-rich plasma: a case study. J Surg Trauma 2020;8(2):73-8. [DOI:10.32592/jsurgery.2020.8.2.104]
7. Mayoly A, Iniesta A, Curvale C, Kachouh N, Jaloux C, Eraud J et al. Development of Autologous Platelet-Rich Plasma Mixed-Microfat as an Advanced Therapy Medicinal Product for Intra-Articular Injection of Radio-Carpal Osteoarthritis: From Validation Data to Preliminary Clinical Results. Int J Mol Sci 2019;20:1111. [DOI:10.3390/ijms20051111] [PMID] [PMCID]
8. Sheykhhasan M, Mohammadi S, Nikbakht M, Ghiasi M, The use of platelet-rich plasma in intervertebral disc regeneration: a review of preclinical studies and clinical experiments. Razi J Med Sci 2017; 24 (156):72-92. Persian. [Google Scholar]
9. Nurden AT, Nurden P, Sanchez M, Andia I, Anitua E. Platelets and wound healing. Front Biosci 2008;13:3532-48. [DOI:10.2741/2947] [PMID]
10. Piersma SR, Broxterman HJ, Kapci M, de Haas RR, Hoekman K, Verheul HM, et al. Proteomics of the TRAP-induced platelet releasate. J Proteomics 2009;72:91-109. [DOI:10.1016/j.jprot.2008.10.009] [PMID]
11. Macaulay IC, Carr P, Gusnanto A, Ouwehand WH, Fitzgerald D, Watkins NA. Platelet genomics and proteomics in human health and disease. J Clin Invest 2005;115:3370-7. [DOI:10.1172/JCI26885] [PMID] [PMCID]
12. Reed GL. Platelet secretory mechanisms. Semin Thromb Hemost 2004;30:441-50. [DOI:10.1055/s-2004-833479] [PMID]
13. Morel O, Toti F, Hugel B, Bakouboula B, Camoin-Jau L, Dignat-George F et al. Procoagulant microparticles: disrupting the vascular homeostasis equation? Arterioscler Thromb Vasc Biol 2006;26:2594-604. [DOI:10.1161/01.ATV.0000246775.14471.26] [PMID]
14. Ghiasi M, Mehdizadeh M, Sharifi A. M, Tafvizi F, Farzaneh S, Maroof N. T. Use of mesenchymal adult stem cell for cartilage regeneration by hydrogel. Int J Med Lab 2019;6: 207-18. [DOI:10.18502/ijml.v6i3.1403]
15. Andrae J, Gallini R, Betsholtz C. Role of platelet-derived growth factors in physiology and medicine. Genes Dev 2008;22:1276-1312. [DOI:10.1101/gad.1653708] [PMID] [PMCID]
16. Graham S, Leonidou A, Lester M, Heliotis M, Mantalaris A, Tsiridis E. Investigating the role of PDGF as a potential drug therapy in bone formation and fracture healing. Expert Opin Investig Drugs 2009;18:1633-54. [DOI:10.1517/13543780903241607] [PMID]
17. Barrientos S, Stojadinovic O, Golinko MS, Brem H, Tomic-Canic M. Growth factors and cytokines in wound healing. Wound Repair Regen 2008;16:585-601. [DOI:10.1111/j.1524-475X.2008.00410.x] [PMID]
18. Ruiz de Almodovar C, Lambrechts D, Mazzone M, Carmeliet P. Role and therapeutic potential of VEGF in the nervous system. Physiol Rev 2009;89:607-48. [DOI:10.1152/physrev.00031.2008] [PMID]
19. Mackenzie F, Ruhrberg C. Diverse roles for VEGF-A in the nervous system. Development 2012;139:1371-80. [DOI:10.1242/dev.072348] [PMID]
20. Kim I, Mogford JE, Chao JD, Mustoe TA. Wound epithelialization deficits in the transforming growth factor-alpha knockout mouse. Wound Repair Regen 2001;9:386-390. [DOI:10.1046/j.1524-475x.2001.00386.x] [PMID]
21. Brown GL, Curtsinger LJ, White M, Mitchell RO, Pietsch J, Nordquist R et al. Acceleration of tensile strength of incisions treated with EGF and TGF-beta. Ann Surg 1988;208:788-94. [DOI:10.1097/00000658-198812000-00019] [PMID] [PMCID]
22. Yamamoto H, Gurney ME. Human platelets contain brain-derived neurotrophic factor. J Neurosci 1990;10:3469-78. [DOI:10.1523/JNEUROSCI.10-11-03469.1990] [PMID] [PMCID]
23. Gray K, Ellis V. Activation of pro-BDNF by the pericellular serine protease plasmin. FEBS Lett 2008;582:907-10. [DOI:10.1016/j.febslet.2008.02.026] [PMID]
24. Pang PT, Teng HK, Zaitsev E, Woo NT, Sakata K, Zhen S et al. Cleavage of proBDNF by tPA/plasmin is essential for long-term hippocampal plasticity. Science 2004;306:487-91. [DOI:10.1126/science.1100135] [PMID]
25. Barde YA, Edgar D, Thoenen H. Purification of a new neurotrophic factor from mammalian brain. EMBO J 1982; 1:549-553. [DOI:10.1002/j.1460-2075.1982.tb01207.x] [PMID] [PMCID]
26. Zacchigna S, Lambrechts D, Carmeliet P. Neurovascular signalling defects inneurodegeneration. Nat Rev Neurosci 2008;9:169-181. [DOI:10.1038/nrn2336] [PMID]
27. Nagahara AH, Tuszynski MH. Potential therapeutic uses of BDNF in neurological and psychiatric disorders. Nat Rev Drug Discov 2011;10:209-19. [DOI:10.1038/nrd3366] [PMID]
28. Zuccato C, Cattaneo E. Role of brain-derived neurotrophic factor in Huntington's disease. Prog Neurobiol 2007;81:294-330. [DOI:10.1016/j.pneurobio.2007.01.003] [PMID]
29. Fumagalli F, Molteni R, Calabrese F, Maj PF, Racagni G, Riva MA. Neurotrophic factors in neurodegenerative disorders: potential for therapy. CNS Drugs 2008;22:1005-19. [DOI:10.2165/0023210-200822120-00004] [PMID]
30. Tapia-Arancibia L, Aliaga E, Silhol M, Arancibia S. New insights into brain BDNF function in normal aging and Alzheimer disease. Brain Res Rev 2008;59:201-20. [DOI:10.1016/j.brainresrev.2008.07.007] [PMID]
31. Fujimura H, Altar CA, Chen R, Nakamura T, Nakahashi T, Kambayashi J et al. Brain-derived neurotrophic factor is stored in human platelets and released by agonist stimulation. Thromb Haemost 2002;87:728-34 [DOI:10.1055/s-0037-1613072] [PMID]
32. Lommatzsch M, Niewerth A, Klotz J, Schulte-Herbruggen O, Zingler C, Schuff-Werner P, et al. Platelet and plasma BDNF in lower respiratory tract infections of the adult. Respir Med 2007;101:1493-9. [DOI:10.1016/j.rmed.2007.01.003] [PMID]
33. Mazzucco L, Balbo V, Cattana E, Guaschino R, Borzini P. Not every PRP-gel is born equal. Evaluation of growth factor availability for tissues through four PRP-gel preparations: Fibrinet, RegenPRP-Kit, Plateltex and one manual procedure. Vox Sang 2009;97:110-8. [DOI:10.1111/j.1423-0410.2009.01188.x] [PMID]
34. Greppi N, Mazzucco L, Galetti G, Bona F, Petrillo E, Smacchia C et al. Treatment of recalcitrant ulcers with allogeneic platelet gel from pooled platelets in aged hypomobile patients. Biologicals 2011;39:73-80. [DOI:10.1016/j.biologicals.2011.01.002] [PMID]
35. Choukroun J, Diss A, Simonpieri A, Girard MO, Schoeffler C, Dohan SL et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part IV: clinical effects on tissue healing. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:e56-e60. [DOI:10.1016/j.tripleo.2005.07.011] [PMID]
36. O'Neill EM, Zalewski WM, Eaton LJ, Popovsky MA, Pivacek LE, Ragno G et al. Autologous platelet-rich plasma isolated using the Haemonetics Cell Saver 5 and Haemonetics MCS+ for the preparation of platelet gel. Vox Sang 2001;81:172-5. [DOI:10.1046/j.1423-0410.2001.00099.x] [PMID]
37. Blair P, Flaumenhaft R. Platelet alpha-granules: basic biology and clinical correlates. Blood Rev 2009;23:177-89 [DOI:10.1016/j.blre.2009.04.001] [PMID] [PMCID]
38. Snyder EL, Calhoun BC. Topical platelet growth factor therapy: of lotions and potions. Transfusion 2001;41:1186-9. [DOI:10.1046/j.1537-2995.2001.41101186.x] [PMID]
39. Zimmermann R, Arnold D, Strasser E, Ringwald J, Schlegel A, Wiltfang J et al. Sample preparation technique and white cell content influence the detectable levels of growth factors in platelet concentrates. Vox Sang 2003;85:283-9. [DOI:10.1111/j.0042-9007.2003.00361.x] [PMID]
40. Sheykhhasan M, Tabatabaei Qomi R, Kalhor N, Mehdizadeh M, Ghiasi M. Evaluation of the ability of natural and synthetic scaffolds in providing an appropriate environment for growth and chondrogenic differentiation of adipose-derived mesenchymal stem cells. Indian J Orthop 2015;49:561. [DOI:10.4103/0019-5413.164043] [PMID] [PMCID]
41. Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhyi J, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:e45-e50. [DOI:10.1016/j.tripleo.2005.07.009] [PMID]
42. Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhyi J, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part III: leucocyte activation: a new feature for platelet concentrates? Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:e51-e5. [DOI:10.1016/j.tripleo.2005.07.010] [PMID]
43. Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhyi J, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:e37-e44. [DOI:10.1016/j.tripleo.2005.07.008] [PMID]
44. Burnouf T, Lee CY, Luo CW, Kuo YP, Chou ML, Wu YW, et al. Human blood-derived fibrin releasates: composition and use for the culture of cell lines and human primary cells. Biologicals 2012; 40:21-30. [DOI:10.1016/j.biologicals.2011.09.017] [PMID]
45. Bishop CJ, Kim J and Green JJ: Biomolecule delivery to engineer the cellular microenvironment for regenerative medicine. Ann Biomed Eng 2014;42:1557-72. [DOI:10.1007/s10439-013-0932-1] [PMID] [PMCID]
46. Abou Neel EA, Chrzanowski W, Salih VM, Kim HW and Knowles JC: Tissue engineering in dentistry. J Dent 2014;42:915-28. [DOI:10.1016/j.jdent.2014.05.008] [PMID]
47. Miron RJ and Zhang Y: Autologous liquid platelet rich fbrin: A novel drug delivery system. Acta Biomater 2018;75:35-51. [DOI:10.1016/j.actbio.2018.05.021] [PMID]
48. Kobayashi E, Flückiger L, Fujioka-Kobayashi M, Sawada K, Sculean A, Schaller B, Miron RJ. Comparative release of growth factors from PRP, PRF, and advanced-PRF. Clin Oral Investig 2016; 20:2353-60. [DOI:10.1007/s00784-016-1719-1] [PMID]
49. Miron RJ, Fujioka-Kobayashi M, Bishara M, Zhang Y, Hernandez M and Choukroun J. Platelet-rich fibrin and soft tissue wound healing: A systematic review. Tissue Eng Part B Rev 2017;23:83-99. [DOI:10.1089/ten.teb.2016.0233] [PMID]
50. Alsousou J, Ali A, Willett K, Harrison P. The role of platelet-rich plasma in tissue regeneration. Platelets 2013; 24:173-82. [DOI:10.3109/09537104.2012.684730] [PMID]
51. Bhujbal R, A Malik N, Kumar N, Kv S, I Parkar M, Mb J. Comparative evaluation of platelet rich plasma in socket healing and bone regeneration after surgical removal of impacted mandibular third molars. J Dent Res Dent Clin Dent Prospect 2018;12:153-8. [DOI:10.15171/joddd.2018.024] [PMID] [PMCID]
52. Del Fabbro M, Bucchi C, Lolato A, Corbella S, Testori T, Taschieri S. Healing of postextraction sockets preserved with autologous platelet concentrates. A systematic review and meta-analysis. J Oral Maxillofac Surg 2017;75:1601-15. [DOI:10.1016/j.joms.2017.02.009] [PMID]
53. Boateng JS, Matthews KH, Stevens HN, Eccleston GM. Wound healing dressings and drug delivery systems: A review. J Pharm Sci 2008; 97:2892-923. [DOI:10.1002/jps.21210] [PMID]
54. Broughton G II, Janis JE, Attinger CE. The basic science of wound healing. Plast Reconstr Surg 2006;117:12S-34S. [DOI:10.1097/01.prs.0000225430.42531.c2] [PMID]
55. Haugh MG, Murphy CM, O'Brien FJ. Novel freeze-drying methods to produce a range of collagen-glycosaminoglycan scaffolds with tailored mean pore sizes. Tissue Eng Part C Methods 2010;16:887-94. [DOI:10.1089/ten.tec.2009.0422] [PMID]
56. Roy I, Gupta MN. Freeze-drying of proteins: Some emerging concerns. Biotechnol Appl Biochem 2004;39:165-77. [DOI:10.1042/BA20030133] [PMID]
57. Shi L, Li R, Wei S, Zhou M, Li L, Lin F et al: Effects of a protective agent on freeze-dried platelet-rich plasma. Blood Coagul Fibrinolysis 2019;30:58-65. [DOI:10.1097/MBC.0000000000000796] [PMID]
58. Wang Z, Han L, Sun T, Wang W, Li X, Wu B. Preparation and effect of lyophilized platelet-rich fibrin on the osteogenic potential of bone marrow mesenchymal stem cells in vitro and in vivo. Heliyon 2019;5:e02739. [DOI:10.1016/j.heliyon.2019.e02739] [PMID] [PMCID]
59. Li Q, Reed DA, Min L, Gopinathan G, Li S, Dangaria SJ, Li L, Geng Y, Galang MT, Gajendrareddy P et al: Lyophilized platelet-rich fibrin (PRF) promotes craniofacial bone regeneration through Runx2. Int J Mol Sci 2014;15:8509-25. [DOI:10.3390/ijms15058509] [PMID] [PMCID]
60. Sam G, Vadakkekuttical RJ, Amol NV. In vitro evaluation of mechanical properties of platelet-rich fibrin membrane and scanning electron microscopic examination of its surface characteristics. J Indian Soc Periodontol 2015;19:32-36. [DOI:10.4103/0972-124X.145821] [PMID] [PMCID]
61. Suga H, Glotzbach JP, Sorkin M, Longaker MT, Gurtner GC. Paracrine mechanism of angiogenesis in adipose-derived stem cell transplantation. Ann Plast Surg 2014;72:234-41. [DOI:10.1097/SAP.0b013e318264fd6a] [PMID] [PMCID]
62. Klar AS, Güven S, Zimoch J, Zapiórkowska NA, Biedermann T, Böttcher-Haberzeth S et al: Characterization of vasculogenic potential of human adipose-derived endothelial cells in a three-dimensional vascularized skin substitute. Pediatr Surg Int 2016;32:17-27. [DOI:10.1007/s00383-015-3808-7] [PMID]
63. Moon KM, Park YH, Lee JS, Chae YB, Kim MM, Kim DS, Kim BW, Nam SW and Lee JH: The effect of secretory factors of adipose-derived stem cells on human keratinocytes. Int J Mol Sci 2012;13:1239-57. [DOI:10.3390/ijms13011239] [PMID] [PMCID]
64. Chen Y, Niu Z, Xue Y, Yuan F, Fu Y and Bai N: Improvement in the repair of defects in maxillofacial soft tissue in irradiated minipigs by a mixture of adipose-derived stem cells and platelet-rich fbrin. Br J Oral Maxillofac Surg 2014;52:740-5. [DOI:10.1016/j.bjoms.2014.06.006] [PMID]
65. Park CG, Joo MW, Jeong J, Kang YK and Lee DR: Evaluation of the effects of the combination of autologous mesenchymal stem cells and platelet-rich plasma on structural bone allograft healing. Cell Tissue Bank 2017;18:229-38. [DOI:10.1007/s10561-017-9611-z] [PMID]
66. Wolfe PS, Sell SA, Ericksen JJ, Simpson DG, Bowlin GL. The creation of electro spun nanofibers from platelet rich plasma. J Tissue Sci Eng 2011;2:2. [DOI:10.4172/2157-7552.1000107]
67. Sell S.A, McClure M. J, Ayres C. E, Simpson D. G, Bowlin G. L. Preliminary investigation of airgap electrospun silkfbroin-based structures for ligament analogue engineering. J Tissue Sci Eng 2011;22:1253-73. [DOI:10.1163/092050610X504251] [PMID]
68. Yoshimi R, Yamada Y, Ito Kenji, Nakamura S, Abe A, Nagasaka T et al. Self-assembling peptide nanofber scaffolds, platelet-rich plasma, and mesenchymal stem cells for injectable bone regeneration with tissue engineering. J Craniofac Surg 2009;20:1523Y1530 [DOI:10.1097/SCS.0b013e3181b09b7e] [PMID]
69. Kohgo T, Yamada Y, Ito et al K. Bone regeneration with self-assembling peptide nanofiber scaffolds in tissue engineering for osseointegration of dental implants. Int J Periodontics Restorative Dentistry 2011;31:e9-e16. [Google Scholar]
70. Berner A, Boerckel J. D, Saifzadeh S, Steck R, Ren J, Vaquette C, Qiyi J et al. Biomimetic tubular nanofiber mesh and platelet rich plasma-mediated delivery of BMP-7 for large bone defect regeneration Cell Tissue Res 2012;347:603-12. [DOI:10.1007/s00441-011-1298-z] [PMID]
71. Sell S. A, Wolfe P. S, Ericksen J. J, Simpson D. G, Bowlin G. L. Incorporating platelet-rich plasma into electrospun scaffolds for tissue engineering applications. Tissue Eng Part A 2011;17:2723-37. [DOI:10.1089/ten.tea.2010.0663] [PMID] [PMCID]
72. Barnes C. P, Sell S. A, Boland E. D, Simpson D. G, Bowlin G. L. Nanofber technology: designing the next generation of tissue engineering scaffolds. Advanced Drug Delivery Reviews Adv Drug Deliv Rev 2007;10:1413-33. [DOI:10.1016/j.addr.2007.04.022] [PMID]
73. Boland E. D, Matthews J. A, Pawlowski K. J, Simpson D. G, Wnek G. E, Bowlin G. L. Electrospinning collagen and elastin: preliminary vascular tissue engineering. Front Biosci. 2004;1:1422-32. [DOI:10.2741/1313] [PMID]
74. Dutra C. E, Pereira M. M, Serakides R, Rezende C. M. In vivo evaluation of bioactive glass foams associated with platelet-rich plasma in bone defects. J Tissue Eng Regen Med 2008;2:221-7. [DOI:10.1002/term.86] [PMID]
75. Zhang Y.-D, Wang G, Sun Y, Zhang C.-Q. Combination of platelet-rich plasma with degradable bioactive borate glass for segmental bone defect repair. Acta Orthop Belg 2011;77:110-5. [Google Scholar]
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