Volume 32, Issue 11 (Februery 2022)
Studies in Medical Sciences 2022, 32(11): 840-846 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Sadeghi E, Nasimfar A, Karamyyar M, Balaneji S. EVALUATION OF SEROPREVALENCE OF MYCOPLASMA PNEUMONIAE SPECIFIC IGM AND IGG ANTIBODIES IN ASYMPTOMATIC PRESCHOOL-AGED CHILDREN. Studies in Medical Sciences 2022; 32 (11) :840-846
URL: http://umj.umsu.ac.ir/article-1-5740-en.html
URL: http://umj.umsu.ac.ir/article-1-5740-en.html
Associate Professor of Pediatric Infectious Diseases, Urmia University of Medical Sciences, Urmia, Iran (Corresponding Author) , sadeghi.e@umsu.ac.ir
Abstract: (1663 Views)
Background & Aims: Mycoplasma pneumoniae is one of the main causes of community acquired pneumonia in children with an incidence of nearly 40%.
The aim of this study was to evaluate the serum levels of IgM and IgG antibodies to identify Mycoplasma pneumoniae in laboratory samples of in asymptomatic preschool aged children in Urmia, Iran.
Materials & Methods: The present cross-sectional study was performed on 260 preschool children who referred to the pediatric clinic of Shahid Motahari Hospital in Urmia in January 2018 without any respiratory symptoms. Detection of IgM and IgG antibodies against Mycoplasma pneumonia was performed on the samples using specific ELISA kit, according to the manufacturer's instructions. IgM and IgG antibody titers more than 12 U / mL considered as positive, less than 8 U / mL as negative, and between 8 and 12 U / mL as intermediate result (. SPSS 21 software was used for data analysis.
Results: According to the results of this study, 25.8 percent of children had at least one episode of mycoplasma pneumonia infection. Statistically significant relationship was detected between the positivity of IgG and IgM and attending kindergarten in the studied children (P=0.001). There was no significant relationship between positivity of IgG and IgM with sex (P values = 0.14 and 0.60, respectively), age (P values = 0.58 and 0.54, respectively), and age group (P values = 0.19 and 0.49, respectively) of the children.
Conclusion: There was a significant relationship between positive IgG and IgM antibody titers and presence in kindergarten, but there was no significant relationship between IgG and IgM antibody positivity with sex and age group.
The aim of this study was to evaluate the serum levels of IgM and IgG antibodies to identify Mycoplasma pneumoniae in laboratory samples of in asymptomatic preschool aged children in Urmia, Iran.
Materials & Methods: The present cross-sectional study was performed on 260 preschool children who referred to the pediatric clinic of Shahid Motahari Hospital in Urmia in January 2018 without any respiratory symptoms. Detection of IgM and IgG antibodies against Mycoplasma pneumonia was performed on the samples using specific ELISA kit, according to the manufacturer's instructions. IgM and IgG antibody titers more than 12 U / mL considered as positive, less than 8 U / mL as negative, and between 8 and 12 U / mL as intermediate result (. SPSS 21 software was used for data analysis.
Results: According to the results of this study, 25.8 percent of children had at least one episode of mycoplasma pneumonia infection. Statistically significant relationship was detected between the positivity of IgG and IgM and attending kindergarten in the studied children (P=0.001). There was no significant relationship between positivity of IgG and IgM with sex (P values = 0.14 and 0.60, respectively), age (P values = 0.58 and 0.54, respectively), and age group (P values = 0.19 and 0.49, respectively) of the children.
Conclusion: There was a significant relationship between positive IgG and IgM antibody titers and presence in kindergarten, but there was no significant relationship between IgG and IgM antibody positivity with sex and age group.
Type of Study: Research |
Subject:
Pediatric Infectious Disease
References
1. Jain S, Williams DJ, Arnold SR, Ampofo K, Bramley AM, Reed C, et al. Community-acquired pneumonia requiring hospitalization among U.S. children [J]. N Engl J Med 2015;372(9):835-45. [DOI:10.1056/NEJMoa1405870] [PMID] [PMCID]
2. Zhao M-c, Li G-x, Zhang D, Zhou H-y, Wang H, Yang S, et al. Clinical evaluation of a new single-tube multiplex reverse transcription PCR assay for simultaneous detection of 11 respiratory viruses, Mycoplasma pneumoniae and Chlamydia in hospitalized children with acute respiratory infections. Diagn Microbiol Infect Dis 2017;88:115-9. [DOI:10.1016/j.diagmicrobio.2017.03.010] [PMID] [PMCID]
3. Zhao MC, Wang L, Qiu FZ, Zhao L, Guo WW, Yang S, et al. Impact and clinical profiles of Mycoplasma pneumoniae co-detection in childhood community-acquired pneumonia. BMC Infect Dis 2019 Oct 11;19(1):835. [DOI:10.1186/s12879-019-4426-0] [PMID] [PMCID]
4. Waites KB, Xiao L, Liu Y, Balish MF, Atkinson TP. Mycoplasma pneumoniae from the respiratory tract and beyond. Clin Microbiol Rev 2017;30(3):747-809. [DOI:10.1128/CMR.00114-16] [PMID] [PMCID]
5. Cai ZH, Dai YY, Huang LY, Zhang WS, Guo XG. Diagnosis of mycoplasma pneumoniae by loop-mediated isothermal amplification: systematic review and meta-analysis. BMC Infect Dis 2019 Feb 19;19(1):173. [DOI:10.1186/s12879-019-3799-4] [PMID] [PMCID]
6. Wood PR, Kampschmidt JC, Dube PH, Marianna PC, Paola C, Norma SK, et al. Mycoplasma pneumoniae and health outcomes in children with asthma. Ann Allergy Asthma Immunol 2017;119(2):146-152.e2. [DOI:10.1016/j.anai.2017.05.022] [PMID] [PMCID]
7. Zhang Y, Mei S, Zhou Y, Huang M, Dong G, Chen Z. Cytokines as the good predictors of refractory mycoplasma pneumoniae pneumonia in school-aged children. Sci Rep 2016;6:37037. [DOI:10.1038/srep37037] [PMID] [PMCID]
8. Kashyap S, Sarkar M. Mycoplasma pneumonia: Clinical features and management. Lung India. 2010; 27(2): 75-85. [DOI:10.4103/0970-2113.63611] [PMID] [PMCID]
9. She RC, Thurber A, Hymas WC, Stevenson J, Langer J, Litwin CM, et al. Limited utility of culture for Mycoplasma pneumoniae and Chlamydophila pneumoniae for diagnosis of respiratory tract infections. J Clin Microbiol 2010;48(9):3380-2. [DOI:10.1128/JCM.00321-10] [PMID] [PMCID]
10. Hong S-J. The role of Mycoplasma pneumoniae infection in asthma. Allergy Asthma Immunol Res 2012;4(2):59-61. [DOI:10.4168/aair.2012.4.2.59] [PMID] [PMCID]
11. Waites KB, Talkington DF. Mycoplasma pneumoniae and its role as a human pathogen. Clin Mcrobiol Rev 2004;17(4):697-728. [DOI:10.1128/CMR.17.4.697-728.2004] [PMID] [PMCID]
12. Vervloet LA, Marguet C, Camargos PAM. Infection by Mycoplasma pneumoniae and its importance as an etiological agent in childhood community-acquired pneumonias. Braz J Infect Dis 2007;11(5):507-14. [DOI:10.1590/S1413-86702007000500012] [PMID]
13. McMillan JA, Mycoplasma pneumoniae infection in pediatrics. Seminars in Pediatric Infectious Diseases; 1998: Elsevier. [DOI:10.1016/S1045-1870(98)80061-7]
14. Zhang L, Zong Z-Y, Liu Y-B, Ye H, Lv X-J. PCR versus serology for diagnosing Mycoplasma pneumoniae infection: a systematic review & meta-analysis. Indian J Med Res 2011;134:270-80. [PMID] [PMCID]
15. Narita M. Evaluation of ELISA kits for detection of Mycoplasma pneumoniae--specific IgG, IgA, IgM antibodies on the diagnosis of Mycoplasma pneumoniae infection in children. Kansenshogaku Zasshi 2005;79(7):457-63. [DOI:10.11150/kansenshogakuzasshi1970.79.457] [PMID]
16. Ranjbar R, Halaji M. Epidemiology of Mycoplasma pneumoniae prevalence in Iranian patients: a systematic review and meta-analysis. J Med Microbiol 2019 Nov;68(11):1614-21. [DOI:10.1099/jmm.0.001079] [PMID]
17. Amin M, Soltan Abadi M, Navidifar T, Torabizadeh M, Alavi S M. Detection of Mycoplasma pneumoniae Among Children with Pneumonia Using Bacterial Culture, Polymerase Chain Reaction, and the Enzyme-linked Immunosorbent Assay Techniques in Ahvaz, Iran. Jundishapur J Microbiol 2018; 11(2):e55554. [DOI:10.5812/jjm.55554]
18. Restrepo MI, Reyes LF, Anzueto A. Complication of community-acquired pneumonia (including cardiac complications). Semin Respir Crit Care Med 2016;37:897-904. [DOI:10.1055/s-0036-1593754] [PMID]
19. Arfaatabar M, Aminharati F, Azimi G, Ashtari A, Pourbakhsh SA, Masoorian E, et al. High frequency of Mycoplasma pneumoniae among patients with atypical pneumonia in Tehran, Iran. Germs 2018;8(3):126-33. [DOI:10.18683/germs.2018.1139] [PMID] [PMCID]
20. Ghotaslou R, Sharifi S, Akhi MT, Soroush MH. Epidemiology, clinical features, and laboratory detection of Mycoplasma pneumoniae infection in East Azerbaijan, Iran. Turkish Journal of Medical Sciences 2013;43(4):521-4. [DOI:10.3906/sag-1202-80]
21. Tuuminen T, Varjo S, Ingman H, Weber T, Oksi J, Viljanen M. Prevalence of Chlamydia pneumoniae andMycoplasma pneumoniae Immunoglobulin G and A Antibodies in a Healthy Finnish Population as Analyzed by Quantitative Enzyme Immunoassays. Clin Diagn Lab Immunol 2000;7(5):734-8. [DOI:10.1128/CDLI.7.5.734-738.2000] [PMID] [PMCID]
22. Shenoy VD, Upadhyaya SA, Rao SP, Shobha K. Mycoplasma pneumoniae infection in children with acute respiratory infection. J Trop Pediatr 2005;51(4):232-5. [DOI:10.1093/tropej/fmi008] [PMID]
23. Dashti AS, Hashemi SM, Halaji M, Arjmand R, Shirvani F. Seroprevalence of Mycoplasma pneumoniae Specific IgM and IgG Antibodies in Asymptomatic Preschool Aged Children of Tehran, Iran, 2010. Arch Pediat Infect Dis 2017;5(4):e62412. [DOI:10.5812/pedinfect.62413]
24. Touati A, Pereyre S, Bouziri A, Achour W, Khaldi A, Jaballah NB, et al. Prevalence of Mycoplasma pneumoniae-associated respiratory tract infections in hospitalized children: results of a 4-year prospective study in Tunis. Diagn Microbiol Infect Dis 2010;68(2):103-9. [DOI:10.1016/j.diagmicrobio.2010.05.010] [PMID]
25. Chen Z, Ji W, Wang Y, Yan Y, Zhu H, Shao X, et al. Epidemiology and associations with climatic conditions of Mycoplasma pneumoniae and Chlamydophila pneumoniae infections among Chinese children hospitalized with acute respiratory infections. Ital J Pediatr 2013;39(1):34. [DOI:10.1186/1824-7288-39-34] [PMID] [PMCID]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
