Volume 32, Issue 10 (January 2022)                   Studies in Medical Sciences 2022, 32(10): 745-756 | Back to browse issues page


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Khan mohamadi ghane F, Ahmadi A, Imani M, Shalizar Jalali A. EVALUATION OF IN VITRO FERTILIZATION POTENTIAL AND SPERM PARAMETERS IN ACRYLAMIDE TREATED MICE. Studies in Medical Sciences 2022; 32 (10) :745-756
URL: http://umj.umsu.ac.ir/article-1-5656-en.html
Associate Professor of Anatomical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran (Corresponding Author) , a.ahmady@urmia.ac.ir
Abstract:   (1654 Views)
Background & Aims: Today, with lifestyle changes, the tendency to use packaged food, street food, and fast food has increased, and most of them contain Acrylamide. One of the most important side effects of Acrylamide is toxicity in the reproductive system. In this study, the long-term effects of Acrylamide on in Vitro Fertilization (IVF) and sperm parameters were investigated.
Materials & Methods: Sixteen male mice (age: 6-8 weeks) were divided into two groups. The control group received normal saline (0.2ml/day) and the treatment group received Acrylamide (50mg/kg, 0.2ml/day) orally for 45 days. At the end of the term, sperms were extracted from the tail of the epididymis and fertilization process was performed in HTF + 4mgBSA medium and embryonic growth stages were studied during 120 hours of incubation. Also, sperm count, motility, viability, sperm chromatin quality, and DNA integrity were evaluated. Two proportion methods by Minitab software and T­-­Test by SPSS software were used for statistical analysis (p<0.05).
Results: There was a significant decrease in the percentage of fertilization, 2 cell-embryos and blastocysts resulting from IVF and a significant increase was observed in the number of arrested embryos in treatment group by Acrylamide compared to the control group (p<0.05). Also in treatment group by Acrylamide, sperm count, motility, and viability of sperms, the number of sperms with DNA damage, abnormal morphology, and with immature nucleus showed a significant increase compared to the control group (p<0.05).
Conclusion: The study showed long-term oral administration of Acrylamide has negative effects on fertility potential and sperm parameters.
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Type of Study: Research | Subject: جنین شناسی

References
1. Mantovani A, Maranghi F. Risk assessment of chemicals potentially affecting male fertility. J Contracept 2005; 72(4): 308-1. [DOI:10.1016/j.contraception.2005.04.014] [PMID]
2. Al-Gubory KH. Environmental pollutants and lifestyle factors induce oxidative stress and poor prenatal development. Reprod Biomed Online 2014; 29(1): 17-31. [DOI:10.1016/j.rbmo.2014.03.002] [PMID]
3. Krishnakumar T, Visvanathan R. Acrylamide in food products: A review. J Food Process Technol 2014; 5(7) : 1. [DOI:10.4172/2157-7110.1000344]
4. Friedman M. Acrylamide: inhibition of formation in processed food and mitigation of toxicity in cells, animals, and humans. Food Funct 2015; 6(6): 1752-72. [DOI:10.1039/C5FO00320B] [PMID]
5. Schettgen T, Rossbach B, Kütting B, Letzel S, Drexler H, Angerer J. Determination of haemoglobin adducts of acrylamide and glycidamide in smoking and non-smoking persons of the general population. Int J Hyg Environ Health 2004; 207(6): 531-9. [DOI:10.1078/1438-4639-00324] [PMID]
6. Ahrari Roodi P, Moosavi Z, Afkhami Goli A, Azizzadeh M, Hosseinzadeh H. Histopathological Study of Protective Effects of Honey on Subacute Toxicity of Acrylamide-Induced Tissue Lesions in Rats' Brain and Liver. Iran J Toxicol 2018; 12(3): 1-8. [DOI:10.32598/IJT.12.3.511.1]
7. Bin-Jumah M, Abdel-Fattah A-FM, Saied EM, El-Seedi HR, Abdel-Daim MM. Acrylamide-induced peripheral neuropathy: manifestations, mechanisms, and potential treatment modalities. Environ Sci Pollut Res 2021: 1-16. [DOI:10.1007/s11356-020-12287-6] [PMID]
8. Gur C, Kandemir FM, Darendelioglu E, Caglayan C, Kucukler S, Kandemir O, et al. Morin protects against acrylamide-induced neurotoxicity in rats: an investigation into different signal pathways. Environ Sci Pollut Res 2021: 1-12. [DOI:10.1007/s11356-021-14049-4] [PMID]
9. Tian S-m, Ma Y-x, Shi J, Lou T-y, Liu S-s, Li G-y. Acrylamide neurotoxicity on the cerebrum of weaning rats. Neural Regener Res 2015; 10(6): 938. [DOI:10.4103/1673-5374.158357] [PMID] [PMCID]
10. Seale SM, Feng Q, Agarwal AK, El-Alfy AT. Neurobehavioral and transcriptional effects of acrylamide in juvenile rats. Pharmacol Biochem Behav 2012; 101(1): 77-84. [DOI:10.1016/j.pbb.2011.12.006] [PMID]
11. Wei Q, Li J, Li X, Zhang L, Shi F. Reproductive toxicity in acrylamide-treated female mice. Reprod. Toxicol 2014; 46: 121-8. [DOI:10.1016/j.reprotox.2014.03.007] [PMID]
12. Marchetti F, Lowe X, Bishop J, Wyrobek AJ. Induction of chromosomal aberrations in mouse zygotes by acrylamide treatment of male germ cells and their correlation with dominant lethality and heritable translocations. Environ Mol Mutagen 1997; 30(4): 410-7. https://doi.org/10.1002/(SICI)1098-2280(1997)30:4<410::AID-EM6>3.0.CO;2-M [DOI:10.1002/(SICI)1098-2280(1997)30:43.0.CO;2-M]
13. Aras D, Cakar Z, Ozkavukcu S, Can A, Cinar O. In Vivo acrylamide exposure may cause severe toxicity to mouse oocytes through its metabolite glycidamide. PLoS One 2017; 12(2): e0172026. [DOI:10.1371/journal.pone.0172026] [PMID] [PMCID]
14. Thonning Olesen P, Olsen A, Frandsen H, Frederiksen K, Overvad K, Tjønneland A. Acrylamide exposure and incidence of breast cancer among postmenopausal women in the Danish Diet, Cancer and Health Study. Int J Cancer 2008; 122(9): 2094-100. [DOI:10.1002/ijc.23359] [PMID]
15. Althuis MD, Fergenbaum JH, Garcia-Closas M, Brinton LA, Madigan MP, Sherman ME. Etiology of hormone receptor-defined breast cancer: a systematic review of the literature. Cancer Epidemiol Biomarkers Prev 2004; 13(10): 1558-68. [DOI:10.1158/1055-9965.1558.13.10] [PMID]
16. Ahmadi A, Jamshidi F. Evaluation of the Protective Effect of Satureja khozestanica Essential Oil on the Potential Fertilization in Vitro and the Process of Embryonic Growth in Syrian Mice Under the Oxidative Stress Induced by Nonophenol. Armaghane danesh 2020; 25(2): 201-12. [Google Scholar]
17. Jahangirfard R, Najafi G, Shalizar-jalali A, Ahmadi A, Zadeh-hashem E. Evaluation of CatSper 2 gene expression and embryonic morphological parameters in mice exposed to ethephon. J Sabzevar Univ Med Sci 2021; 28(3): 379-86. [Google Scholar]
18. Pourentezari M, Talebi A, Abbasi A, Khalili MA, Mangoli E, Anvari M. Effects of acrylamide on sperm parameters, chromatin quality, and the level of blood testosterone in mice. Iran J Reprod Med 2014; 12(5): 335. [PMID] [PMCID]
19. Sakamoto J, Hashimoto K. Reproductive toxicity of acrylamide and related compounds in mice_effects on fertility and sperm morphology. Arch Toxicol 1986; 59(4):201-5. [DOI:10.1007/BF00290538] [PMID]
20. Luo Y-S, Long T-Y, Shen L-C, Huang S-L, Chiang S-Y, Wu K-Y. Synthesis, characterization and analysis of the acrylamide-and glycidamide-glutathione conjugates. Chem Biol Interact 2015; 237: 38-46. [DOI:10.1016/j.cbi.2015.05.002] [PMID]
21. Dearfield KL, Douglas GR, Ehling UH, Moore MM, Sega GA, Brusick DJ. Acrylamide: a review of its genotoxicity and an assessment of heritable genetic risk. Fundam. Mol Mech Mutagen 1995; 330(1-2): 71-99. [DOI:10.1016/0027-5107(95)00037-J]
22. Nixon BJ, Stanger SJ, Nixon B, Roman SD. Chronic exposure to acrylamide induces DNA damage in male germ cells of mice. Toxicol Sci 2012; 129(1): 135-45. [DOI:10.1093/toxsci/kfs178] [PMID]
23. Babakhah L, Azad I, Arbabian M, Tavalaee M, Bahadorani M, Nasr-Esfahani M. Influence of Occupational Exposures on Sperm Parameters and Chromatin Structure. Cell Tissue Res 2017; 8(1): 52-68. [Google Scholar]
24. Duan X, Wang Q-C, Chen K-L, Zhu C-C, Liu J, Sun S-C. Acrylamide toxic effects on mouse oocyte quality and fertility in vivo. Sci Rep 2015; 5(1):1-11. [DOI:10.1038/srep11562] [PMID] [PMCID]
25. Zobeiri F, Sadrkhanlou R-A, Salami S, Mardani K, Ahmadi A. The effect of ciprofloxacin on sperm DNA damage, fertility potential and early embryonic development in NMRI mice. Vet Res Forum 2012; 3(2): 131-5. [PMID] [PMCID]
26. Mozaffari AA, Shahrooz R, Ahmadi A, Malekinejad H, Mardani K. Assessment of protective effect of ethyl pyruvate on sperm parameters, and trend of embryological development after in vitro fertilization (IVF) in phenylhydrazine treated mice. Sci J Kurd 2017; 22(1):11-24. [Google Scholar]
27. Khan Mohammadi Ghane F, Ahmadi A, Razi M. Evaluation of protective effects of Ethyl Pyruvate on embryo developing process in in vitro fertilization (IVF) in Cyclophosphamide treated mice. Res J Med Sci 2014; 25(8): 760-8. [Google Scholar]
28. Fajri M, Ahmadi A, Sadrkhanlou R. Protective effects of Equisetum arvense methanolic extract on sperm characteristics and in vitro fertilization potential in experimental diabetic mice: An experimental study. Int J Reprod Biomed 2020; 18(2): 93. [DOI:10.18502/ijrm.v18i2.6415] [PMID] [PMCID]
29. Ahmadi A, Salami S, Ahmadi A. Evaluation of sperm quality, maturation and DNA integrity in adult mice treated with sulpiride. Tehran Univ Med J 2012; 70(4). [Google Scholar]
30. Capuano E, Fogliano V. Acrylamide and 5-hydroxymethylfurfural (HMF): A review on metabolism, toxicity, occurrence in food and mitigation strategies. J Agric Food Chem 2011; 44(4): 793-810. [DOI:10.1016/j.lwt.2010.11.002]
31. Jeng HA, Pan CH, Chao MR, Chiu CC, et al. Sperm quality and DNA integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons. Int J Occup Med Environ Health. 2016; 29(6): 915-26. [DOI:10.13075/ijomeh.1896.00598] [PMID]
32. Jurewicz J, Radwan M, Sobala W, Radwan P, Bochenek M, Hanke W. Effects of occupational exposure - is there a link between exposure based on an occupational questionnaire and semen quality? Syst Biol Reprod Med 2014; 60(4): 227-33. [DOI:10.3109/19396368.2014.907837] [PMID]
33. Nirupama M, Devaki M, Nirupama R, Yajurvedi H. Chronic intermittent stress-induced alterations in the spermatogenesis and antioxidant status of the testis are irreversible in albino rat. J Physiol Biochem 2013; 69(1): 59-68. [DOI:10.1007/s13105-012-0187-6] [PMID]
34. Collodel G, Federico M, Geminiani M, Martini S, Bonechi C, Rossi C, et al. Effect of trans-resveratrol on induced oxidative stress in human sperm and in rat germinal cells. Reprod Toxicol 2011; 31(2): 239-46. [DOI:10.1016/j.reprotox.2010.11.010] [PMID]
35. Agarwal A, Mulgund A, Alshahrani S, Assidi M, Abuzenadah AM, Sharma R, et al. Reactive oxygen species and sperm DNA damage in infertile men presenting with low level leukocytospermia. Reprod Biol Endocrinol 2014; 12(1): 1-8. [DOI:10.1186/1477-7827-12-126] [PMID] [PMCID]
36. Babaei H, Kheirandish R, Ebrahimi L. The effects of copper toxicity on histopathological and morphometrical changes of the rat testes. Asian Pac J Trop Biomed 2012; 2(3): S1615-S9. [DOI:10.1016/S2221-1691(12)60463-8]
37. Yang HJ, Lee SH, Jin Y, Choi JH, Han CH, Lee MH. Genotoxicity and toxicological effects of acrylamide on reproductive system in male rats. J Vet Sci 2005; 6(2): 103-9. [DOI:10.4142/jvs.2005.6.2.103] [PMID]
38. Wang H, Huang P, Lie T, Li J, Hutz RJ, Li K, et al. Reproductive toxicity of acrylamide-treated male rats. Reprod Toxicol 2010; 29(2): 225-30. [DOI:10.1016/j.reprotox.2009.11.002] [PMID]
39. Suzuki N, Sofikitis N. Protective effects of antioxidants on testicular functions of varicocelized rats. Yonago Acta Med 1999; 42(7): 87-94. [Google Scholar]
40. Cocuzza M, Sikka SC, Athayde KS, Agarwal A. Clinical relevance of oxidative stress and sperm chromatin damage in male infertility: an evidence based analysis. Int Braz J Urol 2007; 33(5): 603-21. [DOI:10.1590/S1677-55382007000500002] [PMID]
41. Talebi A, Vahidi S, Aflatoonian A, Ghasemi N, Ghasemzadeh J, Firoozabadi R, et al. Cytochemical evaluation of sperm chromatin and DNA integrity in couples with unexplained recurrent spontaneous abortions. Andrologia 2012; 44: 462-70. [DOI:10.1111/j.1439-0272.2011.01206.x] [PMID]
42. Talebi AR, Khalili MA, Nahangi H, Abbasi A, Anvari M. Evaluation of epididymal necrospermia following experimental chronic spinal cord injury in rat. Iran J Reprod Med 2007; 4; 171-7. [Google Scholar]

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