Volume 31, Issue 9 (December 2020)                   Studies in Medical Sciences 2020, 31(9): 667-679 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

URL: http://umj.umsu.ac.ir/article-1-5280-en.html
Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran (Correspondin Author) , m.peeri@iauctb.ac.ir
Abstract:   (2675 Views)
Background & Aims: Common nutritional mistakes cause inflammation and homeostasis disruption in heart cells. Inflammasome complex is one of the pathways that induces inflammation and degradation of cardiac protein regeneration. The aim of the present study was to investigate changes in NLRP1inflammasome, PI3k, apoptosis, and histopathology of heart tissue following aerobic physical activity and octopamine supplementation in male rats poisoned with deep-fried oil (DFO).
Materials & Methods: 30 Wistar male rats (300 to 350 g) were randomly divided into 5 groups (n = 6): control, deep-fried oil, deep-fried oil + aerobic exercise, deep-fried oil + octopamine supplement (Sup), and deep-fried oil + aerobic exercise + octopamine. During the study, DFO was orally administered to the rats (gavage 10 ml/kg) for 4 weeks (morning). The dose of octopamine was 81 mol/kg (Intraperitoneal injection) and dissolved in 9% normal saline. It was injected into supplement groups 2 hours after the exercise program. The rats in the training group also exercised at a moderate intensity at 50% vo2max in the first week and 65% vo2max in the last week. Changes in NLRP1 gene expression and PI3k protein expression were performed by RT Pcr and IHC. The tunnel assay was also used to evaluate apoptotic cells.
Results: Consumption of DFO caused a significant increase in tissue damage (p = 0.001) and collagen deposition (p = 0.001) to heart tissue. Also, DFO gavage for 4 weeks in rat model increased tissue damage, mRNA NLRP1 inflammasome, and apoptotic cells (p = 0.001) and also significantly decreased protein expression of PI3K (p = 0.001). Examination of tissue changes also revealed that deep-fried oil + octopamine and deep-fried oil + aerobic exercise + octopamine groups showed a significant decrease in collagen deposition (p<0.05). Therapeutic interventions also reduced the levels of mRNA NLRP1 and Apoptosis cell and increased protein expression of PI3K. Most of these changes were related to the deep-fried oil + aerobic exercise + octopamine group (p<0.05).
Conclusion: The results of the present study showed that taking octopamine with aerobic exercise for 4 weeks can control the inflammasome complex (reduction of NLRP1 mRNA) followed by reduction of apoptosis and improvement of cardiac cell regeneration capacity (PI3K protein expression) in the model of nutritional disorders induced by DFO. Therefore, it can be concluded that octopamine supplementation with exercise can have a cardiac protection effect in non-smart nutritional conditions.
Full-Text [PDF 3474 kb]   (779 Downloads)    
Type of Study: Research | Subject: فیزیولوژی

1. Sacks FM, Lichtenstein AH, Wu JH, Appel LJ, Creager MA, Kris-Etherton PM, et al. Dietary fats and cardiovascular disease: a presidential advisory from the American Heart Association. Circulation 2017;136(3):e1-e23. [DOI:10.1161/CIR.0000000000000510] [PMID]
2. Saritas T, Floege J. Cardiovascular disease in patients with chronic kidney disease. Herz 2020; 45:1-7. [DOI:10.1007/s00059-019-04884-0] [PMID]
3. Members WG, Thom T, Haase N, Rosamond W, Howard VJ, Rumsfeld J, et al. Heart disease and stroke statistics-2006 update: a report from the American Heart Association Statistics Committee and Stroke Statistics Subcommittee. Circulation 2006;113(6):e85-e151. [DOI:10.1161/CIRCULATIONAHA.105.171600] [PMID]
4. Choe E, Min D. Chemistry of deep‐fat frying oils. Journal of food science 2007;72(5):R77-R86. [DOI:10.1111/j.1750-3841.2007.00352.x] [PMID]
5. Ng C-Y, Kamisah Y, Faizah O, Jubri Z, Qodriyah HMS, Jaarin K. Involvement of inflammation and adverse vascular remodelling in the blood pressure raising effect of repeatedly heated palm oil in rats. Int J Vasc Med 2012;2012:404025. 6. Srivastava S, Singh M, George J, Bhui K, Saxena AM, Shukla Y. Genotoxic and carcinogenic risks associated with the dietary consumption of repeatedly heated coconut oil. Br J Nutr 2010;104(9):1343-52. [DOI:10.1017/S0007114510002229] [PMID]
6. Srivastava S, Singh M, George J, Bhui K, Shukla Y. Genotoxic and carcinogenic risks associated with the consumption of repeatedly boiled sunflower oil. J Agric Food Chem 2010;58(20):11179-86. [DOI:10.1021/jf102651n] [PMID]
7. Dung C-H, Wu S-C, Yen G-C. Genotoxicity and oxidative stress of the mutagenic compounds formed in fumes of heated soybean oil, sunflower oil and lard. Toxicol In Vitro 2006;20(4):439-47. [DOI:10.1016/j.tiv.2005.08.019] [PMID]
8. Wu S-C, Yen G-C. Effects of cooking oil fumes on the genotoxicity and oxidative stress in human lung carcinoma (A-549) cells. Toxicol In Vitro 2004;18(5):571-80. [DOI:10.1016/j.tiv.2004.01.004] [PMID]
9. Pandey MK, Dhawan A, Das M. Induction of P53, P21Waf1, orinithine decorboxylase activity, and DNA damage leading to cell‐cycle arrest and apoptosis following topical application of repeated fish fried oil extract to mice. Mol Carcinog 2006;45(11):805-13. [DOI:10.1002/mc.20194] [PMID]
10. Mehta U, Swinburn B. A review of factors affecting fat absorption in hot chips. Crit Rev Food Sci Nutr 2001;41(2):133-54. [DOI:10.1080/20014091091788] [PMID]
11. Salter A. Dietary fatty acids and cardiovascular disease. Animal 2013;7(s1):163-71. [DOI:10.1017/S1751731111002023] [PMID]
12. Perez-Herrera A, Rangel-Zuñiga OA, Delgado-Lista J, Marin C, Perez-Martinez P, Tasset I, et al. The antioxidants in oils heated at frying temperature, whether natural or added, could protect against postprandial oxidative stress in obese people. Food Chem 2013;138(4):2250-9. [DOI:10.1016/j.foodchem.2012.12.023] [PMID]
13. Gupta R, Vind SK, Singh SP, Kumar S, Kumar M. The effect of different deep-fried vegetable oil on cardiovascular system in rats model. World Journal of Pharmaceutical Research 2014;3:1130-9. [Google Scholar]
14. Bleda S, de Haro J, Varela C, Esparza L, Ferruelo A, Acin F. NLRP1 inflammasome, and not NLRP3, is the key in the shift to proinflammatory state on endothelial cells in peripheral arterial disease. Int J Cardiol Heart Vasc 2014;172(2):e282-e4. [DOI:10.1016/j.ijcard.2013.12.201] [PMID]
15. Westin GG, Armstrong EJ, Bang H, Yeo K-K, Anderson D, Dawson DL, et al. Association between statin medications and mortality, major adverse cardiovascular event, and amputation-free survival in patients with critical limb ischemia. J Am Coll Cardiol 2014;63(7):682-90. [DOI:10.1016/j.jacc.2013.09.073] [PMID] [PMCID]
16. Ringseis R, Eder K, Mooren FC, Krüger K. Metabolic signals and innate immune activation inPI3k NLRP1obesity and exercise. Exerc Immunol Rev 2015;21:58-68. [URL]
17. Machado MV, Vieira AB, da Conceição FG, Nascimento AR, da Nóbrega ACL, Tibirica E. Exercise training dose differentially alters muscle and heart capillary density and metabolic functions in an obese rat with metabolic syndrome. Exp Physiol 2017;102(12):1716-28. [DOI:10.1113/EP086416] [PMID]
18. Hengstmann J, Konen W, Konen C, Eichelbaum M, Dengler H. The physiological disposition of p-octopamine in man. Naunyn Schmiedebergs Arch Pharmacol 1974;283(1):93-106. [DOI:10.1007/BF00500148] [PMID]
19. Beaumont RE, Cordery P, James LJ, Watson P. Supplementation with a low-dose of octopamine does not influence endurance cycling performance in recreationally active men. J Sci Med Sport 2017;20(10):952-6. [DOI:10.1016/j.jsams.2017.03.007] [PMID]
20. Kianmehr P, Azarbayjani MA, Peeri M, Farzanegi P. Synergic effects of exercise training and octopamine on peroxisome proliferator-activated receptor-gamma coactivator-1a and uncoupling protein 1 mRNA in heart tissue of rat treated with deep frying oil. Biochemistry and biophysics reports 2020;22:100735. [DOI:10.1016/j.bbrep.2020.100735] [PMID] [PMCID]
21. Sujkowski A, Ramesh D, Brockmann A, Wessells R. Octopamine drives endurance exercise adaptations in Drosophila. Cell Rep 2017;21(7):1809-23. [DOI:10.1016/j.celrep.2017.10.065] [PMID] [PMCID]
22. Zhou Z, Wang Y, Jiang Y, Diao Y, Strappe P, Prenzler P, et al. Deep-fried oil consumption in rats impairs glycerolipid metabolism, gut histology and microbiota structure. Lipids Health Dis 2016;15(1):86. [DOI:10.1186/s12944-016-0252-1] [PMID] [PMCID]
23. Bour S, Visentin V, Prévot D, Carpéné C. Moderate weight-lowering effect of octopamine treatment in obese Zucker rats. Cell Physiol Biochem 2003;59(3):175-82. [DOI:10.1007/BF03179913] [PMID]
24. Fillion L, Henry C. Nutrient losses and gains during frying: a review. Int J Food Sci Nutr 1998;49(2):157-68. [DOI:10.3109/09637489809089395] [PMID]
25. Soriguer F, Rojo-Martínez G, Dobarganes MC, García Almeida JM, Esteva I, Beltrán M, et al. Hypertension is related to the degradation of dietary frying oils. Am J Clin Nutr 2003;78(6):1092-7. [DOI:10.1093/ajcn/78.6.1092] [PMID]
26. Che Z, Liu Y, Chen Y, Cao J, Liang C, Wang L, et al. The apoptotic pathways effect of fine particulate from cooking oil fumes in primary fetal alveolar type II epithelial cells. Mutat Res Genet Toxicol Environ Mutagen 2014;761:35-43. [DOI:10.1016/j.mrgentox.2014.01.004] [PMID]
27. Wang W, Wang C, Gong Y, Zhang X. Inhibition of NLRP1 inflammasome might be a novel therapeutic target in the treatment of peripheral arterial disease. International journal of cardiology 2018;256:29. https://doi.org/10.1016/j.ijcard.2018.01.023 https://doi.org/10.1016/j.ijcard.2017.08.021 [DOI:10.1016/j.ijcard.2017.10.125]
28. Yi Y-S. Role of inflammasomes in inflammatory autoimmune rheumatic diseases. Korean J Physiol Pharmacol 2018;22(1):1-15. [DOI:10.4196/kjpp.2018.22.1.1] [PMID] [PMCID]
29. Fann DY-W, Lee S, Manzanero S, Tang S-C, Gelderblom M, Chunduri P, et al. Intravenous immunoglobulin suppresses NLRP1 and NLRP3 inflammasome-mediated neuronal death in ischemic stroke. Cell Death Dis 2013;4(9):e790-e. [DOI:10.1038/cddis.2013.326] [PMID] [PMCID]
30. Thevis M, Koch A, Sigmund G, Thomas A, Schänzer W. Analysis of octopamine in human doping control samples. Biomed Chromatogr 2012;26(5):610-5. [DOI:10.1002/bmc.1705] [PMID]
31. De Oliveira AL, De Paula MN, Comar JF, Vilela VR, Peralta RM, Bracht A. Adrenergic metabolic and hemodynamic effects of octopamine in the liver. Int J Mol Sci 2013;14(11):21858-72. [DOI:10.3390/ijms141121858] [PMID] [PMCID]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Studies in Medical Sciences

Designed & Developed by : Yektaweb