Volume 28, Issue 8 (Monthly_Nov 2017)                   J Urmia Univ Med Sci 2017, 28(8): 33-41 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

BOROUMAND AZAD F, TADAYON K, NOOFELI M, GHADERI R, SEKHAVATI M, SAEDI S, et al . VNTR9 and VNTR10, two newly-found variable-number tandem repeat loci useful in MLVA genotyping of Bordetella pertussis. J Urmia Univ Med Sci. 2017; 28 (8) :33-41
URL: http://umj.umsu.ac.ir/article-1-3908-en.html
Associate Professor of Microbiology, Razi Vaccine and Serum Research Institute, AREEO, Karaj, Iran , mmb093@gmail.com
Abstract:   (1735 Views)
Background & Aims: Bordetella pertussis, the causative agent of whooping cough, continues to infect human hosts even in those populations where infants and children are routinely vaccinated. Causes of pertussis epidemiology are not fully identified unless strains of the pathogen are characterized by molecular means. Golbally, Multi Locus Variable Number of Tandem Repeats analysis (MLVA) has proved very useful in inter-laboratory surveillance of majoriy of world most important bacterial diseases. This work was conducted to improve the current MLVA typing method developed by Schouls in 2004.
Materials & Methods: An in silico search was comparatively conducted on the whole genomes of 5 laboratory/vaccine strains of B. pertussis deposited in the NCBI genome database by Tandem Repeat Finder software. PCR protocols were then adopted to enable simultaneous amplification found loci. A further comparative genomic analysis of 20 world-known B. pertussis strains from diverse spatial and temporal origins was performed using the detected new VNTR loci.
Results: Two polymorphic loci carrying tandem repeats (TRs) with 6 (AAGCCC) and 9 (GGCTGGCCG) nucleotides were detected and designated as VNTR9 and VNTR10, respectively. Application of these on genomic templates from B. pertussis 107 and B. pertussis 509 vaccine strains used by Razi institute in manufacturing the pertussis vaccine resulted in succesful production of PCR amplicons from both strains. Nei's diversity indices of 0.38 and 0.1 were achieved by these loci, respectively in comparative genomic analysis of B. pertussis strains from across the world.
Conclusion: We assume inclusion of VNTR9 and VNTR10 in MLVA analysis of clinical isolates of B. pertussis is useful in improving current understanding of pertussis in Iran.
Full-Text [PDF 402 kb]   (351 Downloads)    
Type of Study: Research | Subject: میکروبیولوژی

References
1. Sedighi I, Karimi A, Amanati A. Old Disease and New Challenges: Major Obstacles of Current Strategies in the Prevention of Pertussis. Iran J Pediatr 2016; 26 (4): e5514. [PubMed]
2. Ghorbani G.R. Comparing Seasonal Pattern of Laboratory Confirmed Cases of Pertussis with Clinically Suspected Cases. Osong Public Health Res Perspect 2016; 7 (2): 131-7. [PubMed]
3. Sabbe M, Vandermeulen C. The resurgence of mumps and pertussis. Hum Vaccin Immunother 2016; 12 (4): 955-9. [PubMed]
4. Wensley A. Risk factors for pertussis in adults and teenagers in England. Epidemiol Infect 2017; 145 (5): 1025-36. [PubMed]
5. Wagner B. Genetic Variation of Bordetella pertussis in Austria. PLoS One 2015; 10 (7): e0132623. [PubMed]
6. Gangarosa E.J. Impact of anti-vaccine movements on pertussis control: the untold story. Lancet 1998; 351 (9099): 356-61. [PubMed]
7. Mahieu L. Epidemiology of pertussis in children of Flanders Belgium: can healthcare professionals be involved in the infection? Acta Clin Belg 2014; 69 (2): 104-10. [PubMed]
8. Bart M.J. Global population structure and evolution of Bordetella pertussis and their relationship with vaccination. MBio 2014; 5 (2): e01074. [PubMed]
9. Mooi, F.R., N.A. Van Der Maas, and H.E. De Melker. Pertussis resurgence: waning immunity and pathogen adaptation - two sides of the same coin. Epidemiol Infect 2014; 142 (4): 685-94. [PubMed]
10. Barkoff A.M, Grondahl-Yli-Hannuksela K, He Q. Seroprevalence studies of pertussis: what have we learned from different immunized populations. Pathog Dis 2015; 73 (7) [PubMed]
11. Barkoff A.M. Appearance of Bordetella pertussis strains not expressing the vaccine antigen pertactin in Finland. Clin Vaccine Immunol 2012; 19 (10): 1703-4. [PubMed]
12. Mirzaei B. Isolation of High Level Macrolide Resistant Bordetella pertussis Without Transition Mutation at Domain V in Iran. Jundishapur J Microbiol 2015; 8 (7): e18190. [PubMed]
13. Van der Zee A. Dynamics of the population structure of Bordetella pertussis as measured by IS1002-associated RFLP: comparison of pre- and post-vaccination strains and global distribution. Microbiology 1996; 142 ( Pt 12) 3479-85. [PubMed]
14. Cassiday P.K. Changes in Predominance of Pulsed-Field Gel Electrophoresis Profiles of Bordetella pertussis Isolates, United States, 2000-20. Emerg Infect Dis 2016; 22 (3): 442-8. [PubMed]
15. Borisova O. Characteristics of Bordetelia pertussis strains isolated from pertussis patients in Moscow by using multilocus sequencing. Zh Mikrobiol Epidemiol Immunobiol 2012; (2): 28-34. [PubMed]
16. Jung S.O. Multilocus Sequence Analysis of Housekeeping Genes and Antigenic Determinant Genes in Bordetella pertussis Strains Isolated in Korea. Osong Public Health Res Perspect 2011; 2 (2): 115-26. [PubMed]
17. Du Q. Direct molecular typing of Bordetella pertussis from nasopharyngeal specimens in China in 2012-2013. Eur J Clin Microbiol Infect Dis 2016; 35 (7): 1211-4. [PubMed]
18. Schouls L.M. Multiple-locus variable-number tandem repeat analysis of Dutch Bordetella pertussis strains reveals rapid genetic changes with clonal expansion during the late 1990s. J Bacteriol 2004; 186 (16): 5496-505. [PubMed]
19. Seyed-Mohamadi S. Genetic Characterization of Bacillus anthracis 17 JB strain. Iran J Microbiol 2015; 7 (3): 168-72. [PubMed]
20. Carver T. Artemis: an integrated platform for visualization and analysis of high-throughput sequence-based experimental data. Bioinformatics 2012; 28 (4): 464-9. [PubMed]
21. Untergasser A. Primer3--new capabilities and interfaces. Nucleic Acids Res 2012; 40 (15): e115. [Google Scholar]
22. Najafi Olya Z, Tadayon K, Ghaderi R. A Simplified Van Erth Single Nucleotide Polymorphism (SNP) Typing Method of Bacillus Anthracis Applicable by Traditional Thermocycler Machines. Med Laboratory J 2015; 9 (1): 7. [Google Scholar]
23. Sekhavati M. "In-house" production of DNA size marker from a vaccinal Bacillus anthracis strain. Iran J Microbiol 2015; 7 (1): 45-9. [PubMed]
24. Stucky B.J. SeqTrace: a graphical tool for rapidly processing DNA sequencing chromatograms. J Biomol Tech 2012; 23 (3): 90-3. [PubMed]
25. Li W. The EMBL-EBI bioinformatics web and programmatic tools framework. Nucleic Acids Res 2015; 43 (W1): W580-4. [PubMed]
26. Cho S, Whittam TS, Boxrud DJ, Bartkus JM, Saeed AM. Allele distribution and genetic diversity of VNTR loci in Salmonella enterica serotype Enteritidis isolates from different sources. BMC Microbiol 2008;8:146. [PubMed]
27. Rume F.I., Genotype Analysis of Bacillus anthracis Strains Circulating in Bangladesh. PLoS One 2016; 11 (4): e0153548. [PubMed]
28. Vluggen C. Molecular epidemiology of Mycobacterium tuberculosis complex in Brussels, 2010-2013. PLoS One 2017; 12 (2): e0172554. [PubMed]
29. Jang Y. Isolation of Mycobacterium bovis from Free-Ranging Wildlife in South Korea. J Wildl Dis 2017; 53 (1): 181-5. [PubMed]
30. Hornstra H. Molecular epidemiology of glanders, Pakistan. Emerg Infect Dis 2009; 15 (12): 2036-9. [PubMed]
31. Vogler AJ, Nottingham R, Busch JD, Sahl JW, Shuey MM, Foster JT, et al. VNTR diversity in Yersinia pestis isolates from an animal challenge study reveals the potential for in vitro mutations during laboratory cultivation. Infect Genet Evol 2016;45:297–302. [PubMed]
32. Guigon G, Cheval J, Cahuzac R, Brisse S. MLVA-NET--a standardised web database for bacterial genotyping and surveillance. Euro Surveill 2008;13(19). [PubMed]
33. Kurniawan J, Maharjan RP, Chan WF, Reeves PR, Sintchenko V, Gilbert GL, et al. Bordetella pertussis clones identified by multilocus variable-number tandem-repeat analysis. Emerging Infect Dis 2010;16(2):297–300. [PubMed]

Add your comments about this article : Your username or Email:
CAPTCHA code

Send email to the article author


© 2018 All Rights Reserved | URMIA MEDICAL JOURNAL

Designed & Developed by : Yektaweb