Volume 24, Issue 9 (Monthly_Nov 2013)                   J Urmia Univ Med Sci 2013, 24(9): 711-718 | Back to browse issues page

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Hashemnia S M R, Kheradmand F, Noori F, Roshan-Milani S. INHIBITION OF GROWTH FACTOR SIGNALING PATHWAYS BY IMATINIB MESYLATE IN MOUSE NORMAL LEYDIG CELLS. J Urmia Univ Med Sci. 2013; 24 (9) :711-718
URL: http://umj.umsu.ac.ir/article-1-1927-en.html
Assistant Professor of Clinical Biochemistry Cellular and molocular research center of Urmia University of Medical Sciences , fkheradmand@yahoo.com
Abstract:   (5457 Views)

  Background & Aims : Cancer cells proliferation may be mediated by abnormal phosphorylation of signaling pathways downstream of tyrosine kinase receptors such as Platelet derived growth factor receptor α (PDGFR-α) and β (PDGFR-β). We aimed to study the phosphorylation level of PDGFR-α and PDGFR-β and apoptosis in mouse normal leydig cells being exposed to Imatinib.

  Materials & Methods : The mouse TM3 leydig cells were treated with 0, 2.5,5,10 and 20 μM Imatinib for 2, 4, and 6 days. The apoptosis and phosphorylation level of PDGFRs were assessed by caspase-3 activities colorimetric and fluorescence immunoassay methods, respectively. For statistical analysis, one-way ANOVA and T-test were performed.

  Results : Phosphorylation level of PDGFR-α in the treated (0.21±0.001) and control cells (0.35±0.13) was significantly different (P<0.05), and its level decreased with increasing drug dosage (P<0.05). PDGFR-β level and apoptosis had no significant differences between groups, although PDGFR-β level decreased significantly with increasing exposure duration (P<0.05).

  Conclusion : By inhibition of signaling pathways downstream of growth factors specifically PDGFR-α phosphorylation blockage in normal leydig cells, Imatinib may interfere with cellular growth. It seems that this drug has no effect on apoptotic pathways.

  SOURCE: URMIA MED J 2013: 24(9): 718 ISSN: 1027-3727

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Type of Study: Research | Subject: آناتومی

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