Volume 34, Issue 5 (August 2023)
Studies in Medical Sciences 2023, 34(5): 235-246 |
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Research code: 4023، 4018
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Forouhandeh H, Nejadali A, Abdi P, Mehdizadeh Aghdam E, Eliasifar B, Dilmaghani A. ISOLATION OF THE BACTERIA PRODUCING PHYTASE, BETA-GLUCANASE, CELLULASE AND GLUTAMINASE FROM SALINE SOIL OF SEMNAN PROVINCE, IRAN. Studies in Medical Sciences 2023; 34 (5) :235-246
URL: http://umj.umsu.ac.ir/article-1-5973-en.html
URL: http://umj.umsu.ac.ir/article-1-5973-en.html
Haleh Forouhandeh 
, Amin Nejadali , Payman Abdi , Elnaz Mehdizadeh Aghdam , Babak Eliasifar , Azita Dilmaghani * 
, Amin Nejadali , Payman Abdi , Elnaz Mehdizadeh Aghdam , Babak Eliasifar , Azita Dilmaghani *
Associate Professor of Pharmaceutical Biotechnology, Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran (Corresponding Author) , dilmaghania@tbzmed.ac.ir
Abstract: (1537 Views)
Background & Aim: Today, attention has been paid to the enzymes of halophilic microorganisms and their biotechnological applications. One of the most important applications of halophilic microorganisms is their use in the production of hydrolytic enzymes that can perform reactions in harsh conditions. This study was conducted to isolate halophilic bacteria producing phytase, glucanase, cellulase and glutaminase from the saline soil of Semnan province, Iran.
Materials & Methods: In this research study, samples were taken from the saline soil of Haj Aligholi and Biyarajmand in Semnan province, Iran. First, isolated halophilic bacteria were cultured and screened for producing hydrolytic enzymes including phytase, cellulose, glutaminase, and beta-glucanases using enzyme-specific media. The enzymatic activities of the bacteria were then determined based on the formation of a clear halo or the formation of sediment around the colonies after adding the relevant reagents. Afterward, enzyme-positive isolates were identified using 16S rRNA gene sequencing analysis. Finally, the best activity and stability of the enzymes produced at different pH and temperatures were investigated.
Results: Results showed that the isolated halophilic bacteria were able to produce hydrolytic enzymes. The halophilic bacteria producing cellulose, glutaminase, and beta-glucanase enzymes belong to the Bacillus genus. The optimum pH and temperature for the highest activity of cellulase were 8 and 40 °C, respectively, and the enzyme stability occurred in pH= 8 at 60 °C. In addition, the optimal activity of glutaminase occurred at pH= 8 and 50 °C, and the best pH and temperature for glutaminase stability were 8 and 60 °C, respectively. Halophilic bacteria that produce beta-glucanase showed favorable growth at pH=9 and 50°C.
Conclusion: The results of the study showed that the bacteria isolated from the soil are of the genus Bacillus which can produce all three enzymes, beta-glucanase, cellulose, and glutaminase, and can be good sources for the production of enzymes necessary for research and industrial work.
Materials & Methods: In this research study, samples were taken from the saline soil of Haj Aligholi and Biyarajmand in Semnan province, Iran. First, isolated halophilic bacteria were cultured and screened for producing hydrolytic enzymes including phytase, cellulose, glutaminase, and beta-glucanases using enzyme-specific media. The enzymatic activities of the bacteria were then determined based on the formation of a clear halo or the formation of sediment around the colonies after adding the relevant reagents. Afterward, enzyme-positive isolates were identified using 16S rRNA gene sequencing analysis. Finally, the best activity and stability of the enzymes produced at different pH and temperatures were investigated.
Results: Results showed that the isolated halophilic bacteria were able to produce hydrolytic enzymes. The halophilic bacteria producing cellulose, glutaminase, and beta-glucanase enzymes belong to the Bacillus genus. The optimum pH and temperature for the highest activity of cellulase were 8 and 40 °C, respectively, and the enzyme stability occurred in pH= 8 at 60 °C. In addition, the optimal activity of glutaminase occurred at pH= 8 and 50 °C, and the best pH and temperature for glutaminase stability were 8 and 60 °C, respectively. Halophilic bacteria that produce beta-glucanase showed favorable growth at pH=9 and 50°C.
Conclusion: The results of the study showed that the bacteria isolated from the soil are of the genus Bacillus which can produce all three enzymes, beta-glucanase, cellulose, and glutaminase, and can be good sources for the production of enzymes necessary for research and industrial work.
Type of Study: Research |
Subject:
میکروبیولوژی
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