Volume 33, Issue 12 (March 2023)
Studies in Medical Sciences 2023, 33(12): 868-875 |
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Ethics code: IR.IAU.PIAU.REC.1401.002
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Moayeri M, Nezhadali M, Mohamadi M. ASSOCIATION BETWEEN FAT MASS AND OBESITY ASSOCIATED (FTO) RS17817449 GENE POLYMORPHISM WITH INSULIN RESISTANCE AND OBESITY IN THE PATIENTS WITH NON-ALCOHOLIC FATTY LIVER DISEASE. Studies in Medical Sciences 2023; 33 (12) :868-875
URL: http://umj.umsu.ac.ir/article-1-5903-en.html
URL: http://umj.umsu.ac.ir/article-1-5903-en.html
Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran , moayeri110@gmail.com
Abstract: (758 Views)
Background & Aims: Non-alcoholic fatty liver disease (NAFLD) is now the most common liver disease that affects a quarter of global population. NAFLD is associated with metabolic disorders such as type 2 diabetes, high blood pressure, obesity, and cardiovascular diseases. The aim of this study was to investigate the association of FTO (fat mass and obesity associated) rs17817449 gene polymorphism with insulin resistance and obesity in the people with NAFLD.
Materials & Methods: This case-control study was conducted on 80 patients with non-alcoholic fatty liver disease and 80 participants as controls. Insulin concentration was measured by ELISA kit and the other variables were determined by standard methods. Genotyping was done by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. Data were analyzed using SPSS version 20 software at significance level below 0.05.
Results: The results showed that all the variables except LDL, fasting plasma glucose (FPG) and cholesterol had significant association between control and NAFLD group (P<0.05). However, no associations were found between rs17817449 polymorphism with Body Mass Index and HOMA, and genotype frequencies of rs17817449 was not significantly different between obese and normal subjects, as well as between insulin-resistant and insulin-sensitive groups (P>0.05).
Conclusion: Nonalcoholic fatty liver disease is associated with Body Mass Index and insulin resistance. The FTO rs17817449 polymorphism is not associated with obesity and insulin resistance.
Materials & Methods: This case-control study was conducted on 80 patients with non-alcoholic fatty liver disease and 80 participants as controls. Insulin concentration was measured by ELISA kit and the other variables were determined by standard methods. Genotyping was done by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. Data were analyzed using SPSS version 20 software at significance level below 0.05.
Results: The results showed that all the variables except LDL, fasting plasma glucose (FPG) and cholesterol had significant association between control and NAFLD group (P<0.05). However, no associations were found between rs17817449 polymorphism with Body Mass Index and HOMA, and genotype frequencies of rs17817449 was not significantly different between obese and normal subjects, as well as between insulin-resistant and insulin-sensitive groups (P>0.05).
Conclusion: Nonalcoholic fatty liver disease is associated with Body Mass Index and insulin resistance. The FTO rs17817449 polymorphism is not associated with obesity and insulin resistance.
Keywords: Fat Mass and Obesity Associated Gene, Insulin Resistance, Non-Alcoholic Fatty Liver, Obesity, Rs17817449
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