Volume 32, Issue 6 (September 2021)
Studies in Medical Sciences 2021, 32(6): 468-476 |
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Shabestarian H, Homayouni Tabrizi M, Eshaghi A. Evaluation of Antioxidant and Antibacterial Effects of PLGA Nanoparticles Loaded with Rapeseed Pollen Extract. Studies in Medical Sciences 2021; 32 (6) :468-476
URL: http://umj.umsu.ac.ir/article-1-5501-en.html
URL: http://umj.umsu.ac.ir/article-1-5501-en.html
Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran (Corresponding author)
Abstract: (2194 Views)
Background & Aims: In this study, the antioxidant and antibacterial capacity of PLGA nanoparticles containing rapeseed extract (RE-PNP) was investigated.
Materials & Methods: Three various methods including Disk Diffusion (DD), Minimal Inhibitory Concentration (MIC), and Minimum Bactericidal Concentration (MBC) were used to evaluate the antibacterial effects of synthesized nanoparticles against different strains of bacteria. The inhibition capacity of ABTS and DPPH free radicals was measured to evaluate the antioxidant power of RE-PNP.
Results: The results showed that the RE-PNP have the potential to inhibit DPPH radicals (IC50 = 500μg / ml) and ABTS (IC50 = 1000μg / ml). The inhibitory effect of RE-PNP on the growth of Staphylococcus aurous and Micrococcus luteus was confirmed by the growth inhibition zone 8 and 15 in the disk diffusion model.
Conclusion: According to the results, RPE-PNPs can be used as a safe, natural, and effective antibiotic for bacterial infections caused by Staphylococcus aureus and Micrococcus luteus and also, this formulation can be used due to its antioxidant effects in treatment of oxidative stress-related diseases.
Materials & Methods: Three various methods including Disk Diffusion (DD), Minimal Inhibitory Concentration (MIC), and Minimum Bactericidal Concentration (MBC) were used to evaluate the antibacterial effects of synthesized nanoparticles against different strains of bacteria. The inhibition capacity of ABTS and DPPH free radicals was measured to evaluate the antioxidant power of RE-PNP.
Results: The results showed that the RE-PNP have the potential to inhibit DPPH radicals (IC50 = 500μg / ml) and ABTS (IC50 = 1000μg / ml). The inhibitory effect of RE-PNP on the growth of Staphylococcus aurous and Micrococcus luteus was confirmed by the growth inhibition zone 8 and 15 in the disk diffusion model.
Conclusion: According to the results, RPE-PNPs can be used as a safe, natural, and effective antibiotic for bacterial infections caused by Staphylococcus aureus and Micrococcus luteus and also, this formulation can be used due to its antioxidant effects in treatment of oxidative stress-related diseases.
Keywords: PLGA nanoparticle, Rapeseed pollen, ABTS, DPPH, Disk Diffusion test, Minimal inhibitory concentration (MIC), Minimum Bactericidal concentration (MBC)
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