Volume 33, Issue 6 (September 2022)                   Studies in Medical Sciences 2022, 33(6): 404-412 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

URL: http://umj.umsu.ac.ir/article-1-5484-en.html
Department of Chemistry, Payame Noor University, 4697-19395, Tehran, Iran , zareanalyst@gmail.com
Abstract:   (1252 Views)
Background & Aims: Silver nanoparticles are among the most important and widely used nanoparticles used in biological researchs. Synthesis of silver nanoparticles by green method has various advantages that make these nanoparticles useful for interdisciplinary studies. Leukemias are one of the most common malignancies among children, and various methods have been introduced to treat them. The aim of this study was to investigate the cytotoxic effect of silver nanoparticles synthesized with Thymus vulgaris plant extract on Molt-4 leukemic cell line, which belongs to acute lymphoid leukemias and is used for in vitro studies.
Materials & Methods: In this study, we first synthesized silver nanoparticles with Thymus vulgaris extract using green synthesis method. Then, the nanoparticles were tested and approved by D.L.S technology. The synthesized nanoparticles along with the extract of Thymus vulgaris plant were exposed to Molt-4 cells at 48 and 72 hours. The amount of cytotoxicity was analyzed by MTT test, and the amount of necrosis and apoptosis in the cells was checked by acridine orange/ethidium bromide staining method.
Results: The obtained information indicated the successful synthesis of silver nanoparticles by the extract of Thymus vulgaris plant. The size of the synthesized nanoparticles was between 20 and 35 nanometers. The IC50 value for Molt-4 cells also decreased with increasing time. Also, Thymus vulgaris extract and nanoparticles induced primary and secondary apoptosis as well as necrosis in Molt-4 cells.
Conclusion: This study confirms the effect of nanoparticles and Thymus vulgaris plant on induction of cell death in the studied cells and introduces a potential factor for the treatment of these diseases.
Full-Text [PDF 823 kb]   (907 Downloads)    
Type of Study: Research | Subject: General

1. Khan I, Saeed K, Khan I. Nanoparticles: Properties, applications and toxicities. Arab J Chem 2019;12(7):908-31. [DOI:10.1016/j.arabjc.2017.05.011]
2. Sahayaraj K, Rajesh S. Bionanoparticles: synthesis and antimicrobial applications. Science against microbial pathogens: communicating current research and technological advances. 2011;23:228-44. [Google Scholar]
3. Yuan Y-G, Zhang S, Hwang J-Y, Kong I-K. Silver nanoparticles potentiates cytotoxicity and apoptotic potential of camptothecin in human cervical cancer cells. Oxid Med Cell Longev 2018;2018. [DOI:10.1155/2018/6121328] [PMID] [PMCID]
4. Siddiqi KS, Husen A, Rao RA. A review on biosynthesis of silver nanoparticles and their biocidal properties. J Nanobiotechnology 2018;16(1):1-28. [DOI:10.1186/s12951-018-0334-5] [PMID] [PMCID]
5. Deshmukh S, Patil S, Mullani S, Delekar S. Silver nanoparticles as an effective disinfectant: A review.Mater Sci Eng C 2019;97:954-65. [Google Scholar]
7. Yadi M, Mostafavi E, Saleh B, Davaran S, Aliyeva I, Khalilov R, et al. Current developments in green synthesis of metallic nanoparticles using plant extracts: a review. Artif Cells Nanomed Biotechnoly 2018;46(sup3):S336-S43. [DOI:10.1080/21691401.2018.1492931] [PMID]
8. Andrivon D, Bardin M, Bertrand C, Brun L, Daire X, Fabre F, et al. Can organic agriculture give up copper as a crop protection product? Synthesis of the scientific assessment report: Institut National de la Recherche Agronomique (INRA); 2018. [URL]
9. Adamczyk M, Grabarczyk M, Leszko W. A voltammetric approach to the quantification of tungsten in environmental waters using a solid bismuth microelectrode. Measurement (Lond) 2022;194(111089):111089. Available from: http://dx.doi.org/10.1016/j.measurement.2022.111089. [DOI:10.1016/j.measurement.2022.111089]
10. Seyfried TN, Huysentruyt LC. On the origin of cancer metastasis. Crit Rev Oncog 2013;18(1-2). [DOI:10.1615/CritRevOncog.v18.i1-2.40] [PMID] [PMCID]
11. Guan X. Cancer metastases: challenges and opportunities. Acta Pharm Sin B. 2015;5(5):402-18. [DOI:10.1016/j.apsb.2015.07.005] [PMID] [PMCID]
12. Behrmann L, Wellbrock J, Fiedler W. Acute myeloid leukemia and the bone marrow niche-take a closer look. Front Oncol 2018;8:444. [DOI:10.3389/fonc.2018.00444] [PMID] [PMCID]
13. Ratan ZA, Haidere MF, Nurunnabi M, Shahriar SM, Ahammad AJS, Shim YY, et al. Green chemistry synthesis of silver nanoparticles and their potential anticancer effects. Cancers (Basel) 2020;12(4):855. Available from: http://dx.doi.org/10.3390/cancers12040855. [DOI:10.3390/cancers12040855] [PMID] [PMCID]
14. Liu D, Yang Y, Liu Q, Wang J. Inhibition of autophagy by 3-MA potentiates cisplatin-induced apoptosis in esophageal squamous cell carcinoma cells. Med Oncol Med 2011;28(1):105-11. [DOI:10.1007/s12032-009-9397-3] [PMID]
15. Cavalieri E, Frenkel K, Liehr JG, Rogan E, Roy D. Chapter 4: estrogens as endogenous genotoxic agents-DNA adducts and mutations. JNCI Monographs 2000;2000(27):75-94. [DOI:10.1093/oxfordjournals.jncimonographs.a024247] [PMID]
16. Teoh F, Pavelka N. How chemotherapy increases the risk of systemic candidiasis in cancer patients: current paradigm and future directions. Pathogens 2016;5(1):6. [DOI:10.3390/pathogens5010006] [PMID] [PMCID]
17. Elshafie H, Armentano M, Carmosino M, Bufo S, De Feo V, Camele I. Cytotoxic activity of Origanum vulgare L. on hepatocellular carcinoma cell line HepG2 and evaluation of its biological activity. Molecules 2017;22(9):1435. Available from: http://dx.doi.org/10.3390/molecules22091435. [DOI:10.3390/molecules22091435] [PMID] [PMCID]
18. Ahmad, H, Safiyeh G. The study of Thymus vulgaris Cytotoxicity effects on breast cancer cell's line. J Sabzevar Univ Med Sci 2014; 21 (1): 122-130. [Google Scholar]
19. Hemmati S, Joshani Z, Zangeneh A, Zangeneh MM. Green synthesis and chemical characterization of Thymus vulgaris leaf aqueous extract conjugated gold nanoparticles for the treatment of acute myeloid leukemia in comparison to doxorubicin in a leukemic mouse model. Appl Organomet Chem 2020;34(2):e5267. [DOI:10.1002/aoc.5267]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Studies in Medical Sciences

Designed & Developed by : Yektaweb