Volume 32, Issue 6 (September 2021)                   Studies in Medical Sciences 2021, 32(6): 399-407 | Back to browse issues page


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Mota A, Aboutalebi Vand Beilankouhi E, Mohammad Alizadeh fard H, khodabandeh Z, Mirzamohammadi F, Valilo M. INVESTIGATION OF PARAOXONASE1 ACTIVITY RATIO TO ARYL ESTERASE OF PARAOXONASE 1 ENZYME AND FREQUENCY OF N-ACETHYLTRANSFERASE-2 ENZYME POLYMORPHISMS IN PATIENTS WITH PARKINSON'S DISEASE: A CASE-CONTROL STUDY. Studies in Medical Sciences 2021; 32 (6) :399-407
URL: http://umj.umsu.ac.ir/article-1-4941-en.html
MSc of Clinical Biochemistry, Tabriz University of Medical Science, Tabriz, Iran (Corresponding Author) , valilo.biomed@gmail.com
Abstract:   (2093 Views)
Background & Aims: Parkinson's disease is the second most commonly diagnosed neurological disease after Alzheimer's disease. Paraoxonase 1 enzyme is an antioxidant enzyme with two paraoxonase and aryl esterase activity, and the change in activity and concentration of this enzyme can contribute to Parkinson's disease. The N-acetyltransferase enzyme plays an important role in the detoxification of organophosphates, in which the N-acetyltransferase 2 enzyme plays an important role. The aim of this study was to evaluate the paraoxonase activity ratio to aryl esterase activity of paraoxonase enzyme and the frequency of N-acetyltransferase 2 enzyme polymorphisms in patients with Parkinson's disease.
Materials & Methods: In this case-control study, 80 cases (40 patients with Parkinson's disease and 40 control subjects) were studied. N-acetyltransferase-2 polymorphisms by the PCR-RFLP method were investigated using the relevant bound restriction enzymes. The serum level of paraoxonase 1 was determined by ELISA and enzyme activity of paraoxonase 1 was measured with spectrophotometric.
Results: The ratio of paraoxonase to Aryl esterase did not show any significant difference between the two groups (p >0.05). Significant differences were observed in the frequency of m1 polymorphism genotypes between control and patient groups (p <0.05). However, the prevalence of polymorphism genotypes m1 and m2 between the two groups did not show a significant difference (p> 0.05).
Conclusion: The results of this study showed that three N-acetyltransferase 2 polymorphisms, m1 polymorphism, as well as a decrease in serum levels and paraoxonase 1 activity, may contribute to the pathogenesis and etiology of Parkinson's disease.
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Type of Study: case report | Subject: Neuroscience

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