دوره 27، شماره 12 - ( ماهنامه اسفند 1395 )                   جلد 27 شماره 12 صفحات 1058-1067 | برگشت به فهرست نسخه ها



DOI: 10.18869/acadpub.umj.27.12.1058

XML English Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Amirsasan R, Esmaeili A, Dabbagh Nikokheslat S, Karimi P. The effect of Aerobic Training on Serotonin and Tryptophan Hydroxylase of Prefrontal Cortex in type 2 Diabetic Rats. J Urmia Univ Med Sci. 2017; 27 (12) :1058-1067
URL: http://umj.umsu.ac.ir/article-1-3433-fa.html
Amirsasan Ramin، Esmaeili Ameneh، Dabbagh Nikokheslat Saeed، Karimi Pouran. The effect of Aerobic Training on Serotonin and Tryptophan Hydroxylase of Prefrontal Cortex in type 2 Diabetic Rats. مجله پزشکی ارومیه. 1395; 27 (12) :1058-1067

URL: http://umj.umsu.ac.ir/article-1-3433-fa.html


دانشجوی دکتری فیزیولوژی ورزشی دانشگاه تبریز، تبریز ایران ، ameneh.esmaeili@yahoo.com
چکیده:   (1515 مشاهده)

Background & Aims: Type 2 diabetes (T2D) is a self-management disease and depression is a common problem related to it. One of the causes of depression is serotonin (5-HT) depleted. The enzyme tryptophan hydroxylase (TPH) is known as limiting enzyme in the production of 5-HT in the brain. Aerobic exercise also has proven benefits in treating and reducing the incidence of chronic diseases such as diabetes. Thus, in this study, we examine the effect of aerobic training on 5-HT and TPHof prefrontal cortex in type 2 diabetic rats.

Materials & Methods: This study is experimental and post-test. 30 rats were randomly divided into 3 groups: 1- healthy control  2-diabetic control and 3- exercise diabetic. Groups 2 and 3 received streptozotocin (37mg/kg) by intraperitoneal injection two weeks after the high-fat diet Diabetic training 5 times a week for 8 weeks run on a treadmill with duration and intensity that in the final weeks were 55 min / d  and 26 m / min  , respectively. 24 hours after the last exercise the prefrontal cortex of mice tissue samples of all groups were extracted and 5-HT (µg/g) and TPHconcentration was measured respectively by Elisa and Western Blotting from prefrontal cortex tissue samples. To evaluate the differences between the group of design, analysis of variance (ANOVA) and Tukey post hoc test at the significance level was less than 0.05 were used.

Results: Statistical analysis showed that 5-HT levels in the diabetic group were significantly lower than the healthy control group (P=0.001) and exercise diabetic (P=0.009) and average 5-HT between control group and exercise diabetic has no significant difference. TPH results show that the average diabetic groups were significantly lower than the healthy control group (P=0.000). The results showed that the amount of TPH in the exercise diabetic group was significantly higher than the diabetic control group (P=0.000).

Conclusion: In this study, diabetes reduces 5-HT in the prefrontal cortex. Some studies have shown that inflammation in type 2 increases the cytokines IL-6 and TNF-α, and these cytokines by increasing the activity of  indolamine 2, 3 dioxygenase in the brain alters the metabolism of tryptophan and reduces the production of 5-HT. Chronic activity reduces systemic and tissue inflammation, thus increasing 5-HT in the brain. The reduction of TPH due to diabetes can also be the factors that affect in the decrease of prefrontal 5-HT.

متن کامل [PDF 320 kb]   (535 دریافت)    
نوع مطالعه: پژوهشي(توصیفی- تحلیلی) | موضوع مقاله: فیزیولوژی ورزشی
دریافت: ۱۳۹۵/۳/۲۶ | پذیرش: ۱۳۹۵/۵/۴ | انتشار: ۱۳۹۵/۱۲/۲۲

ارسال نظر درباره این مقاله : نام کاربری یا پست الکترونیک شما:
کد امنیتی را در کادر بنویسید

ارسال پیام به نویسنده مسئول


کلیه حقوق این وب سایت متعلق به مجله پزشکی ارومیه می باشد.

طراحی و برنامه نویسی : یکتاوب افزار شرق

© 2015 All Rights Reserved | URMIA MEDICAL JOURNAL

Designed & Developed by : Yektaweb