Volume 26, Issue 2 (Monthly_April 2015)                   Stud Med Sci 2015, 26(2): 156-165 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Toudehrousta M, Gheibi N, Ilghari D, Sirati-Sabet M. THE EFFECT OF NICKEL ON THE STRUCTURE OF KINASE REGION OF TYPE 2 FIBROBLAST GROWTH FACTOR RECEPTOR. Stud Med Sci. 2015; 26 (2) :156-165
URL: http://umj.umsu.ac.ir/article-1-2782-en.html
Qazvin University of Medical Sciences, Qazvin, Iran , gheibi.n@yahoo.com
Abstract:   (6632 Views)

Background & Aims: Fibroblast growth factor receptor type II (FGFR2b) is the essential factor of cellular signal transduction that regulates important biological processes including cell proliferation and differentiation. The inpairment in the signaling of these receptors is associated with several human pathology. Various factors including toxic metals can change the signaling pathways. This study was aimed to investigate the effects of Nickel as the toxic metal on the structure of FGFR2b.

Materials & Methods: In this study, by using plasmid pLEICS-01, BL21 bacterial, induction of IPTG, electrophoresis and column containing Ni2+ -NTA the recombinant FGFR2b was expressed and purified. The effects of Ni concentrations under 100 mM on the structure of the protein kinase domain by Spectrofluorometer and Circular dichroism (CD) was studied.

Resultas: The nickel concentrations (under 100 mM) do not have a significant impact on the regular secondary structure of the protein. But they show a minor change in natural emission of tryptophan in the tertiary structure.

Conclusion: The main structure and stability kinase domain of the fibroblast growth factor receptor type II does not change by nickel.


SOURCE: URMIA MED J 2015: 26(2): 165 ISSN: 1027-3727

Full-Text [PDF 719 kb]   (1381 Downloads)    
Type of Study: Research | Subject: آناتومی

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2022 CC BY-NC 4.0 | Studies in Medical Sciences

Designed & Developed by : Yektaweb