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EFFECT OF FIBROBLAST AND ENDOTHELIAL CELLS CONDITIONED MEDIA ON PHENOTYPE AND FUNCTION OF DENDRITIC CELLS AND POLARIZED T LYMPHOCYTES RESPONSE
Meysam Ganji Bakhsh, Dr Vahid Nejati, Masoumeh Asadi, Dr Norouz Delirezh, Dr Farah Farokhi ,
Volume 22, Issue 6 (Biomonthly Feb-Mar 2012)
Abstract

  

 Background & Aims: Dendritic cells (DCs) have high ability in antigen presentation. Their functional importance is in treatment of some diseases such as cancer and infection and autoimmune diseases, and prevention of allograft rejection. The aim of this study was to investigate maturation of Dendritic cells for tumor immunotherapy.

 Materials & Methods: DCs was produced in two stages. In the first stage monocyte cells were converted to immature dendritic cells in presence of GM-CSF and IL-4. In the second stage DCs became mature in presence of maturation factor of skin fibroblast cells (HSFPI3) and human umbilical vein endothelial cells (HUVEC) conditioned medium and Poly I-C, MCM. Extract of cancer cells (k562) was added as the antigen to the immature DCS.

 Results: The generated DCs had appropriate phenotype, phagocytosis ability, and stimulate proliferation of T lymphocytes and were able to secrete high levels of IL-12 cytokine.

 Conclusion: The DCs generated in presence of endothelial and fibroblast cells had appropriate had the appropriate phenotype and functional and polarizated T lymphocytes type 1 (Th1) immuny response.   

  SOURCE : URMIA MED J 2012: 22(6): 584 ISSN: 1027-3727


INVESTIGATION OF THE EFFECT OF COPPER OXIDE NANOPARTICLES ON THE EFFICACY OF METHOTREXATE AND PACLITAXEL IN MCF-7 BREAST CANCER CELLS
Sara Khezri, Farah Farokhi, Yaghub Pazhang,
Volume 35, Issue 8 (November 2024)
Abstract
Background & Aims: Breast cancer is one of the most common types of cancer in women, resulting from the uncontrolled growth of cells in breast tissue. This study aims to investigate the impact of copper oxide nanoparticles on the efficacy of the chemotherapeutic agents methotrexate and paclitaxel in reducing the growth of MCF-7 breast cancer cells.
Materials & Methods: In this study, MCF-7 cell lines were treated with copper oxide nanoparticles and chemotherapeutic drugs in RPMI-1640 culture medium supplemented with 10% FBS. Cells were exposed to various concentrations of copper oxide nanoparticles (1, 2, 3, 4 µmol/mL), methotrexate (10, 20, 30, 40 µmol/mL), and paclitaxel (2, 4, 6, 8 µmol/mL) for durations of 24, 48, and 72 hours. Cell viability was assessed using the MTT assay, while apoptosis was measured through Hoechst staining and flow cytometry.
Results: The results indicated that the combination of copper oxide nanoparticles with methotrexate and paclitaxel significantly reduced cell growth compared to each drug used alone. The combination of copper oxide nanoparticles with a concentration of 40 μM methotrexate and a concentration of 8 μM paclitaxel showed the greatest reduction in cell growth, equivalent to a 70% and 75% reduction, respectively. Hoechst staining and flow cytometry indicated that the combined treatment increased cell apoptosis by 56.7%.
Conclusion: These findings suggest that copper oxide nanoparticles have potential as an effective agent in breast cancer treatment. The combination of these nanoparticles with methotrexate and paclitaxel may enhance therapeutic efficacy, providing new strategies to address existing challenges in cancer treatment.
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