Volume 33, Issue 2 (May 2022)                   Studies in Medical Sciences 2022, 33(2): 116-130 | Back to browse issues page

Ethics code: IR.UMSU.REC.1399.068

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PHD student of exercise physiology, Department of Exercise Physiology, Faculty of Sports Sciences, Urmia University, Urmia, Iran (Corresponding author) , mehdi.jafarlu@gmail.com
Abstract:   (611 Views)
Background & Aims: Formation of free radicals in the kidney cells of diabetics leads to apoptosis of them and eventually nephropathy. The aim of this study was to investigate the effects of intervals and aerobic exercise along with astaxanthin supplementation on the expression levels of BAD, BAX and Bcl2 genes in the kidney tissue of rats with type 2 diabetes.
Materials & Methods: 35 male Wistar rats with an average weight of 197.46±15.55 grams were randomly divided into two non-diabetic (5 healthy controls) and diabetic (30) groups, and after induction of diabetes, they were randomly divided again in 6 groups (with 5 rats in each group) including diabetic control, diabetes+supplement, diabetes+interval, diabetes+supplement+interval, diabetes+aerobic, diabetes+aerobic+supplement. The exercise intervention groups performed the program of aerobic and interval exercises for eight weeks as five days per week. Supplement groups received 3 mg of astaxanthin supplement per kilogram of body weight dissolved in 0.3 ml of olive oil by gavage every day. The expression levels of BAD, BAX, and Bcl2 genes were measured in the kidney tissue of rats with type 2 diabetes. One-way analysis of variance and Tukey's post hoc test were used for data analysis.
Results: All exercise intervention protocols and astaxanthin supplementation had significant effects on the BAD index in the kidney tissue of the type 2 diabetic rats (p=0.001). All groups, except endurance training (p=0.890), had significant effects on BAX index in the kidney tissue of type 2 diabetic rats (p=0.001). Also, the use of astaxanthin supplement along with endurance training caused significant changes in Bcl2 index compared to single endurance training (p=0.001).
Conclusion: The intervention of aerobic and interval exercises with or without astaxanthin supplementation in the eight-week intervention period has positive effects on the apoptotic biomarkers in the kidney tissue of rats with type 2 diabetes.
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Type of Study: Clinical trials | Subject: Exercise physiology

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