Dr Gholamhossein Javanmard,
Volume 22, Issue 3 (Biomonthly July-August 2011)
Abstract
Elmira Javanmardi, Dr Ehsan Saboory , Dr Mohamad Hasan Khadem Ansari ,
Volume 22, Issue 3 (Biomonthly July-August 2011)
Abstract
Background &Aims: Several studies indicate that feeding with breast milk is associated with reduction in risk of obesity later in life. Children fed with breast milk have better regulation of nutrient intake in adulthood. The aim of this study was to investigate the impact of offspring's gender and age on maternal Leptin blood levels and effects of mothers' physical activity and contraceptive methods on specific gravity of breast milk.
Materials & Methods: In this study, 115 mothers referring to health centers of Urmia were selected randomly. A questioner was filled out for each mother asking about sex, age, weight of offspring's as well as information about method of delivery and contraception of mother. Five ml of breast milk and blood sample were taken from each mother and were used to measure the Specific Gravity (SG) and blood Leptin levels (BLL), respectively.
Results: Though changes in BLL significantly differed according to offspring's age, differences in BLL were not significant according to offspring's gender. Physical activity of mother significantly increased SG of mother's milk. Differences in SG of maternal milk were significant based on contraceptive technique. There was no significant correlation between BLL and SG of milk in mothers.
Conclusion: Maternal BLL can change with offspring's age. Since Leptin is a major regulator of energy intake, children fed with breast milk might grow up and gain weight better than babies fed with formula. Also, that daily maternal activity in normal life affects quantity and quality of breast milk.
Source: Urmia Med J 2011: 22(3): 279 ISSN: 1027-3727
Dr Sara Javanmardi , Dr Farshid Sarrafzadeh-Rezaei, Dr Noruoz Delirezh, Dr Rahim Hobbenaghi,
Volume 22, Issue 6 (Biomonthly Feb-Mar 2012)
Abstract
Background & Aims : Injuries to tendons are among the most common injuries to body. A repaired tendon needs to be immobilized for weeks until it has accrued enough strength to handle physiological loads. Tissue-engineered cell therapies offer many new treatment options for repair of diseased and damaged tissues. The present study aimed to investigate the effect of intratendinous injection of bMSCs on the rate and extent of early stages of tendon healing after primary repair in a rabbit model.
Materials & Methods : Twenty seven skeletally mature New Zealand white rabbits weighing 1.8- 2.5 kg were used. Twenty rabbits were used as the experimental animals, and seven others were used as a source of allogenic bMSCs. Under general anesthesia an experimental tenotomy was made through the midsubstance of the DDF tendon. The transected tendon was immediately repaired with use of a locking-loop suture. No treatment was given to the control group. In the treatment group, bMSCs injected intartendinously at the repair site as well as externally around the repair site. The operated limbs were immobilized for two weeks post operatively. Samples from operated tendons were harvested at weeks of three of operation for biomechanical (tensile strength, yield point and stiffness) and immunohistochemical (type and extent of produced collagens) evaluations.
Results : Biomechanical findings revealed that there were significant improvements in biomechanical properties such as tensile strength and yield point (P<0.05). Immunohistochemical findings showed that three weeks after surgery production of collagen type Ι around the repair site in the bMSCs treated group was significantly denser and more regular than in the control group.
Conclusion : Intratendinous application of bone marrow-derived mesenchymal stem cells following primary tendon repair can significantly improve the biomechanical and immunohistochemical parameters in the early stage of tendon healing. Early time period during tendon healing is crucial in the treatment of tendon injuries.
SOURCE: URMIA MED J 2012: 22(6): 588 ISSN: 1027-3727
Dr Fariba Abbasi, Dr Zahra Yekta, Dr Farahnaz Noroozinia , Dr Morteza Motazakker, Dr Farzaneh Javanmard ,
Volume 24, Issue 1 (Apr-May 2013)
Abstract
Abstract
Background & Aims : Frozen section is a high- risk procedure with important consequences. As the histopathologic diagnosis of the frozen section may have serious consequences for the treatment of the patient, a high degree of accuracy is mandatory and quality control is important. The aim of this study was the evaluation of accuracy of frozen section by revision of slides in teaching hospitals of Urmia University of Medical Sciences including Imam Khomeini and Shahid Motahari hospitals.
Methods & Materials : In this cross sectional study, the slides of all frozen section samples in teaching hospitals of Urmia University of Medical Sciences during 2003 to 2009 revised by two pathologists and compared with their final permanent section diagnoses.
Results : A total of 155 neoplastic specimens in Imam Khomeini hospital and 83 neoplastic specimens in Shahid Motahari hospital were studied. Sensitivity, Specificity, positive and negative predictive values and accuracy in Iman Khomeini hospital were 95.6%, 100%, 100%, 96.5% and 96.7% and in Shahid Motahari hospital 100%, 98%, 96.2% , 100% and 98.9% , respectively. We had 8 discordant diagnoses, 3 cases (37.5%) were due to macroscopic discordance, 1 case (12.5%) of microscopic discordance and 5 cases (62.5%)of both macroscopic and microscopic discordance.
Conclusion : As different studies showed accuracy of 91.5% to 97.4% for frozen section so we concluded that frozen sections are interpreted with acceptable accuracy in Urmia University Of Medical Sciences.
SOURCE: URMIA MED J 2013: 24(1): 76 ISSN: 1027-3727
Alireza Vali, Saleh Rasras, Fateme Javanmardi Ghahdrijani ,
Volume 25, Issue 3 (Monthly_June 2014)
Abstract
Background & Aims: Different hormonal responses may appear in severe head injured patients. These changes can also be seen in recovery and rehabilitation periods, so influencing the patients, outcome. This study compared changes in thyroid hormones in patients undergoing craniotomy have been due to a severe head injury with craniotomy patients have not been done.
Materials & Methods: In this clinical trial, 59 patients following severe brain injury (GCS ≤ 8) were admitted to Ahwaz Golestan Hospital neurosurgery service and were studied regarding inclusion and exclusion criteria. The patients were divided into two groups of 27 patients. The first group patients who had focal lesions underwent brain surgery craniotomy. First test was taken 24 hours and second sample was taken after day 5. The serum sample was tested using ELISA and human fully automatic machines. The data were analyzed with SPSS software and using independent t-test, Pearson correlation coefficient (P <0.05)
Results: The mean serum T3 and TSH levels in patients with severe head trauma craniotomy showed statistically no significant differences compared with patients who had no craniotomy. And there was no connection between relief and serum levels of these variables. There were relationships between healing and T4 levels in both groups of patients.
Conclusion: The results of this study showed that craniotomy surgery was effective in improving patients with severe head trauma and hormone thyroxine (T4) was used as a prognostic factor in these patients.
SOURCE: URMIA MED J 2014: 25(3): 198 ISSN: 1027-3727
Bagher Pourheydar , Maryam Shahi, Gholam Hossein Farjah, Masoome Javanmard, Mojtaba Karimipour , Fatemeh Atabaki,
Volume 25, Issue 7 (Monthly_sep 2014)
Abstract
Background & Aims: Cerebral ischemia is known as a major worldwide problem and subsequent reperfusion leads to apoptosis or programmed cell death. Specific regions of the brain and specific types of neurons, including hippocampal CA1 pyramidal neurons are more sensitive in cerebral ischemia. Today cell therapy is one of the common treatments that spread among the researchers. In this study, we evaluated apoptosis in hippocampal cells of rat following intravenous injection of bone marrow stromal cells (BMSCs) in ischemia-reperfusion model.
Materials & Methods: In this study, adult male wistar rats (n=40) weighting (250-300g) were used. The rats were divided into the five groups of 8 animals including: control, sham, ischemia, vehicle, and treatment. In the sham group surgery was performed without blocking common carotid arteries. In ischemia group common carotid arteries blocked for twenty minutes and then allowed to reperfusion. In the vehicle group 7 days after ischemia, 30µl PBS was injected via tail vein. In the treatment group BMSC cells (800000/ 30 µl suspension) were injected into the tail vein 7 days after ischemia. And 72 hours before transplantation, the cells were labeled with Brdu. 12 days after ischemia, rats were fixed with 4% Paraformaldehyde through transcardial perfusion and their brains were removed. After histological processing, 5 micron sections were prepared for staining. For immunohistochemistry study anti-Brdu anti-body was used and BMSC cells were identified. Apoptotic cells were detected by TUNEL staining.
Results: Sever apoptosis was observed in ischemia group. The mortality rate in the group which was treated with BMSC was lower.
Conclusion: Ischemia-reperfusion for 20 minutes causes damage and extensive neural death in the hippocampus, especially in CA1 region and injection of bone marrow stromal stem cells significantly decreased the number of apoptotic neurons.
SOURCE: URMIA MED J 2014: 25(7): 597 ISSN: 1027-3727
Elnaz Hosseinalipour, Mojtaba Karimipour, Masoumeh Zirak Javanmard,
Volume 28, Issue 7 (Monthly_Oct 2017)
Abstract
Background & Aims: Cyclophosphamide is an anticancer drug that, despite its many clinical applications, has toxic effects through production of free radicals on body. The aim of this study was to evaluate the protective effects of N-acetylcysteine in the liver tissue of rats treated with cyclophosphamide
Materials & Methods: Twenty-one adult female Wistar rats were randomly divided into 3 groups. The control group received normal saline for 15 days intraperitoneally. Experimental groups1 and 2 received a single dose of cyclophosphamide (150 mg / kg intraperitoneally). 7 days before and 7 days after administration of cyclophosphamide, the mice in these two groups received normal saline and n-acetylcysteine (150 mg / kg). At the end of the study, serum levels of aminotransferase and alanine aminotransferase aspartate were measured and liver tissue was obtained for histopathologic evaluation.
Results: In the treatment group, n-acetylcysteine significantly decreased the amount of elevated aminotransferase and alanine aminotransferase catabolism induced by cyclophosphamide (P <0.05). Administration of cyclophosphamide caused pathological changes in the liver structure, including dilatation of portal veins, infiltration of leukocytes and bulking hepatocytes with fatty vacuoles, and the supplementation of n-acetylcysteine greatly prevented these structural changes in the liver.
Conclusion: The results of this study indicated that the use of n-acetylcysteine can reduce the harmful effects of cyclophosphamide on liver tissue.
Sara Javanmardi, Samira Golmohammadi, Ramin Mazaheri-Khameneh,
Volume 28, Issue 8 (Monthly_Nov 2017)
Abstract
Background & aims: Adhesion and fibrotic bands formation is one of the most important post-surgical complication in pelvic and abdominal surgeries. Major studies in prevention of adhesion formation have based on antioxidant and anti-inflammatory agents. Silymarin, Silybum marianum extract contains compounds with antioxidant, anti-inflammatory and fibrinolytic activities thus could reduce adhesion formation. The aim of the present study was to examine silymarin effects on post –operative peritoneal adhesion prevention in rats.
Materials & Methods: In this study eighteen 8-week-old male Sprague-Dawley rats were used. Under general anesthesia a 2-3 cm ventral midline incision was aseptically made. To induction of adhesions the cecal abrasion model was used. Rats were randomly divided into three groups silymarin, vehicle and the control, each containing 6 rats. Rats in silymarin group received 50 mg/kg silymarin, while rats in the control group were given 0.9% sodium chloride and the vehicle group were treated with equal volume of poly ethylene glycol, intraperitonealy once daily for five days. All animals were sacrificed on the 15th postoperative day. The adhesions were scored as 0, 1, 2 and 3. The cecum and adherent bands were prepared for histopathological evaluation. A semi-quantitive scoring system of the hitopathological grading scale was used for the evaluation of the inflammation grade.
Results: There were no incisional hernias or wound dehiscences in any animals of the three groups. Both macroscopic adhesion scoring and the histopathological grading showed significantly low adhesion scores in the silymarin group in comparision to the control group. (p<0.05)
Conclusion: Based on results, it was concluded that silymarin is effective in preventing postoperative peritoneal adhesions in rats.
Sara Javanmardi, Masoumeh Moradi Arzlou, Seyed Hosein Jarolmasjed,
Volume 28, Issue 9 (Monthly_Dec 2017)
Abstract
Background & Aims: Zeolite has skin wound healing capacity. However, its efficacy on burn wound healing has not yet been examined. In this study, we investigated the effects of Zeolite-Ag+/Gelatin nanocomposite on the process of third-degree burn wound healing in mice model.
Materials & Methods: Ag+- zeolite/gelatin nanocomposite was fabricated by sol-gel method; MTT assay and antimicrobial activity evaluation of the nanocomposite were performed. Third degree burn wound were created on 40 Balb/c mice (n=10) wound per group and treatment administered daily starting on the day of wounding. Treatment groups included untreated control, SSD, gelatin and nanocomposite. Daily photographs were taken and change in wound area relative to initial area was calculated. At 7 and 14 days, animals euthanized and skin samples were taken to histopathologicl evaluation (H&E staining). Then, the study groups were evaluated for wound contraction percent and histopathological parameters.
Results: According to the results of the present study, nano composite-treated wounds revealed significant wound contraction on days 14 compared to control and gelatin treated groups (P<0.0001). Considering investigated histopathological parameters including re-epithelialization angiogenesis and fibroplasia in mice treated with nanocomposite the rate healing of was significantly accelerated when compared to the other groups.
Conclusion: In conclusion, based on the results of the present study, it could be concluded that zeolite-Ag/gelatin nanocomposite offered potential advantages in burn wound healing acceleration and improvement. However, clinical use of this nanocomposite needs more supportive studies.
Masoumeh Zirakjavanmard, Mojtaba Karimipour, Fatemeh . Atabaki Pasdar,
Volume 29, Issue 7 (Monthly_Oct 2018)
Abstract
Background & Aims: One of cell- based technical issues associated with cartilage repair assay is delivering cells to the site of the parts where damage is created. Mesenchymal stem cells (MSCs) with their chondrogenic potential are ideal candidates for cartilage regeneration. High expression of cartilage hypertrophy markers by MSCs would result in apoptosis and ossification. This investigation is attempted to find out if paracrine factors secreted from MSCs can regenerate damaged articular in a rat model.
Materials & Methods: Osteoarthritis (OA) was induced in eighteen adult rats by intra- articular (IA) injection of monosodium iodoacetate (MIA) into right knee. Then the animals were randomly divided into three groups (n=6). Control group received IA injection of vehicle; MSCs group received a single IA injection of MSCs (2.5×105/50µL DMEM) and conditioned medium(CM) group received a single IA injection of CM, meanwhile six left intact knee joints were taken as the normal group. After four weeks, the animals were scarified by overdose injection of ketamine and then, the distal end of femur removed and fixed in formalin. After decalcification using formic acid, histological slides obtained. In this study number of chondrocytes and morphology of articular surface were evaluated.
Results: Four weeks after treatment, gross and microscopic evidence of articular cartilage of distal end of femur cartilage demonstrated the significant (p<0.05) healing of OA joints in group treated with MSCs. Also, in CM group significant increase in number of chondrocyte (p<0.05) and decrease of degenerated holes width was observed compared to the control group.
Conclusion: Given the deficiencies of cell-therapy in OA, a potential for repair of degenerated articular cartilage is thought possible to be represented by cell free CM.
Farzaneh Javanmard, Sonia Sadeghpour, Seyede Zohre Banihashemi,
Volume 32, Issue 2 (May 2021)
Abstract
Heterotopic mesenteric ossification is a rare bone-like lesion located inside the abdominal cavity. Its etiology is unclear but most of the patients had a history of abdominal trauma or surgical operation.
In this study, we present the case of a 29-year old woman admitted with acute abdominal pain and fever that underwent emergency laparotomy. She had a history of recurrent pelvic inflammatory disease and surgical appendectomy. During a laparotomy, a massive left tubo-ovarian abscess was seen and drained out. There was also a hard mass-like lesion measuring 2×1cm attached to the mesentery that was excised and sent for pathologic evaluation. The patient was discharged in good general condition.
Microscopic evaluation of the mass in hematoxylin and eosin stained section revealed a structure consisting of bony trabeculae and fibroblastic proliferation.
This case represents a rare simultaneous occurrence of mesenteric ossification with a ruptured tubo-ovarian abscess that due to a history of recurrent inflammatory disease introduce inflammatory prosess as a possible cause of heterotopic mesenteric ossification.
Fariba Abbasi, Masumeh Pourjabali, Farzaneh Javanmard, Hania Fattahi,
Volume 32, Issue 6 (September 2021)
Abstract
Background & Aims: Histologic tumor grading is a well
-documented useful, inexpensive and easily done prognostic factor but it shows many differences when viewed by multiple pathologists. The aim of the present study was to determine the interobserver differences in tumor grading among pathologists in Urmia, Imam Khomeini Hospital
.
Materials & Methods: In this descriptive, analytical retrospective study, 50 cases of breast carcinoma, urothelial papillary tumor and soft tissue sarcoma were evaluated. Three pathologists reviewed all slides and graded the tumors after codifying and without any knowledge of the opinion of each other. The interobserver concordance was determined by K statistics. A p< 0.05 is considered significant
.
Results: The interobserver concordance was fair in grading of breast, and bladder carcinoma and moderate in soft tissue sarcoma (Kappa was 0.3, 0.13 and 0.603, respectively). Based on the evaluation of factors included in histologic grading, the agreement was higher for pleomorphism in breast cancer, cellular thickness layer in bladder tumor, and cellular differentiation in soft tissue sarcoma (Kappa was 0.8, 0.28 and 0.65, respectively
(.
Conclusion: According to our results, interobserver concordance in the grading of different tumors was fair. To solve this problem, it is suggested to do grading more than one time and in different microscopic fields. Simultaneous grading by two pathologists with multi-head microscope or considering the average of what two pathologists achieve separately can be useful
.
ّfariba Abbasi, Farzaneh Javanmard, Mahsa Esmaiel Fam,
Volume 32, Issue 7 (October 2021)
Abstract
Background & Aims: Cancer is the most common cause of death in developing and developed countries, accounting for nearly 13% of world death. In Iran, gastrointestinal cancers are common. Therefore, this study was conducted to evaluate the gastrointestinal tract tumors in Urmia Imam Khomeini hospital.
Material & Methods: In this cross-sectional study, pathology reports of 741 patients with gastrointestinal tumor were evaluated.
Results: Of 741 reported tumor, 705 cases (95.14%) were malignant, 36 cases (4.85%) were benign. 61.8% of patients with malignant tumors were men and 38.1% were women. The mean age of patients with malignant tumors was 60.2±13.35 and the mean age of patients with benign tumors was 47.7±10.49. The most abundant malignant tumor was adenocarcinoma with 481 cases (68.22%) followed by squamous cell carcinoma with 137 cases (19.43%) and the most frequent benign tumor was lipoma(52.7% of cases). The malignant tumors were observed mostly in the ages of 71 or higher (232 cases (32.9%)). In the evaluation of anatomical location of the malignant tumors, in esophagus, the precise location was not reported in most cases (63.7%). In the stomach, the cardia with 29.27%, in the small intestine, the duodenum with 66.6%, and in the colorectal region, the rectum with 41.9% were the most involved locations.
Conclusion: The results of our study indicated that the most abundant malignant tumor of the gastrointestinal tract was adenocarcinoma and the most abundant benign tumor was lipoma. Malignant tumors were observed mostly in men while benign tumors were mostly observed in women. The high frequency of malignant tumors in the ages of 71 and higher indicates the importance of early-onset screening for the prevention of malignant tumors in older ages.
Farzaneh Javanmard, Sardar Demokri,
Volume 32, Issue 12 (March 2022)
Abstract
Background & Aims: The use of curettage in the diagnosis of endometrial disorders is very widespread, and is the most common diagnostic method before performing hysterectomy. Various studies have been performed to compare the pathological results obtained from curettage with the final pathological results of hysterectomy. The results of some of these studies indicated high efficiency of curettage but the others indicated its inefficiency in the diagnosis of endometrial disorders, especially endometrial carcinoma. The aim of this study was to evaluate the diagnostic accuracy of curettage before hysterectomy in determining tumour type.
Materials & Method: Pathological specimens obtained from curettage and hysterectomy in 89 patients with endometrial cancer who underwent hysterectomy in Shahid Motahari Hospital in Urmia from 2015 to 2018, were reviewed and the data of these two methods were compared. Demographic information of the patients was also extracted from their files and their relationship with the diagnostic accuracy of curettage before hysterectomy in determination of the type of tumor in the patients with endometrial cancer was examined.
Results: Curettage had a high ability to distinguish between malignant and benign forms of the tumor. Due to the positive predictive value and high sensitivity (95.95% and 80.68%, respectively) and high diagnostic accuracy in determination of the tumor type (78.3%), if the curettage result is also positive, curettage indicates malignancy of the tumor with a high confidence level. The diagnostic accuracy of curettage has no significant relationship with age, clinical signs, and tumor type, but is associated with the tumor grade and this association is higher in the tumors with higher FIGO grade than the lower-grade tumors.
Conclusion: Due to its high diagnostic accuracy, curettage is a reliable diagnostic method before performing a hysterectomy.
Farzaneh Javanmard, Javad Rasouli, Fatemeh Azizi,
Volume 34, Issue 1 (April 2023)
Abstract
Background & Aims: Cervical cancer is the fourth most frequently diagnosed cancer in women. Colposcopy is a worldwide accepted method for detection of early carcinoma of cervix. This study was conducted to investigate the correlation between colposcopic examination results with histopathological findings and its diagnostic value in cervical biopsy.
Materials & Methods: This study was a cross-sectional comparative study performed on 436 women with abnormal Pap smear test results, referring to Urmia Motahari Hospital during March 2021 to March 2022. Colposcopy and histopathologic findings of individuals with abnormal Pap smear results were extracted from the patient records. The data were analyzed using descriptive and analytic statistics (Chi-square test) using SPSS 21 (SPSS Inc., Chicago, IL.,USA). The sensitivity, specificity, and positive and negative predictive values of various diagnostic methods were calculated. A probability of P<0.05 was accepted as significant.
Results: The mean age of the participants was 37.3 ± 9.60. The most common finding in colposcopy and histopathology results were acetowhite lesions and cervicitis, respectively. According to the comparison, the agreement percentage of colposcopy and histopathology findings in noninvasive malignant neoplasia in the case of CINIII, CINII, CINI were 4%, 57.1%, and 37%, respectively. Sensitivity, specificity, and positive and negative predictive values for colposcopy in cervical intraepithelial neoplasia were reported as 57.3%, 51.8%, 23.3%, and 82.5%, respectively.
Conclusion: This study showed an acceptable correlation between colposcopic examination and histopathologic findings. Thus colposcopy can be used as a tool for early diagnosis of precancerous cervical lesions as a screening method alongside the pap smear. The correlation between the experience of examiner and accuracy of colposcopy results were not meaningful.
Farzaneh Javanmard, Rahman Khosravi,
Volume 35, Issue 11 (February 2025)
Abstract
Ovarian cysts are one of the most common abdominal masses in female fetuses and infants. Among the ovarian cysts in infants, serous cystadenoma is extremely rare, with only a few reported cases in the literature. Here, we report a case of a neonatal ovarian serous cystadenoma treated with surgical cystectomy and confirmed by histopathological examination. The antenatal ultrasonography (USG) in the third trimester had shown an abdominal cyst on the right side of the abdomen. After birth, the entire cyst was removed during laparotomy. On gross examination, an unilocular cyst measuring 4×3.5×3.5 cm with a wall thickness of 0.3 cm was noted. Microscopic examination revealed epithelial cells with a papillary pattern, regular cell layers, and ischemic changes due to hemorrhagic infarction. This study emphasizes the significance of prenatal evaluation, timely diagnosis, and considering the potential risk of complications.
