Volume 25, Number 12 (Monthly-Feb 2015)                   J Urmia Univ Med Sci 2015, 25(12): 1128-1133 | Back to browse issues page


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Mehrabi M R, Soleimani M, Mirzaee M, Mirshahi M. DIRECT SANDWICH ELISA METHOD FOR DIAGNOSIS OF STOMACH ADENOCARCINOMA. J Urmia Univ Med Sci. 2015; 25 (12) :1128-1133
URL: http://umj.umsu.ac.ir/article-1-2686-en.html

Assistant Professor of Hematology and Blood Banking Department of Laboratory Sciences, Borujerd Branch, Islamic Azad University, Broujerd, Iran , mehrabi.mehr@gmail.com
Abstract:   (3314 Views)

 

Background & Aims: Solid tumors in their course of development have the stages of dysplasia, carcinoma insitu, angiogenesis, invasion and metastasis. In this process, peptide components having the ability of angiogenesis inhibition (angiostatin) are produced and due to their low molecular weight, they are filtrated. The purpose of this study is to study the probability of primary diagnosis of stomach adenocarcinoma in patients by the application of the filtered peptides in urine to introduce a non-invasive and safe method.

Materials & Methods: The randomized urine samples of 20 patients with stomach adenocarcinoma were compared with normal urine samples of 20 healthy people by means of the optimized direct sandwich ELISA in order to diagnose the peptide components.

 Results: The results by means of T-test (P value< 0.05) showed a significant correlation between stomach adenocarcinoma and finding of these peptides in urine and they were completely in accordance with the results of pathologic tests.

Conclusion: The comparison between results obtained in this study showed that by finding the peptides in patients' urine, a non-invasive and safe method was used for primary detection of stomach adenocarcinoma in suspicious patients which was performable before invasive methods (pathology) nevertheless, it is needed to confirm it with complementary tests similar to each screening method.

 

SOURCE: URMIA MED J 2015: 25(12): 1133 ISSN: 1027-3727

Full-Text [PDF 375 kb]   (577 Downloads)    
Type of Study: Research | Subject: آناتومی
Received: 2015/03/3 | Accepted: 2015/03/3 | Published: 2015/03/3

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