Urmia University of Medical Sciences
Studies in Medical Sciences
2717-008X
29
8
2018
11
1
Predictive value of serum cystatin C in kidney recipients with poor early graft ā€ˇfunction
549
556
FA
homayoun
ansari
Department of Clinical Biochemistry, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran
farid
Javandoust Gharehbagh
Department of Clinical Biochemistry, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran
ali
taghizadeh afshari
Center for Nephrology & Kidney Transplantation, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran
hamidreza
khakhali
Department of Epidemiology, School of Medicine,Urmia University of Medical Sciences, Urmia, Iran
jaffar
nouroozzadeh
School of Medicine, Department of Clinical Biochemistry, Urmia University of Medical Sciences, Urmia, Iran
Background & Aims: Despite improvements in surgical techniques and immunosuppression regimen, poor early graft function (PEGF) is a common problem among kidney recipient. PEGF has an effect on the short and long-term graft outcome. Its timely diagnosis, therefore, is of the main priorities. To date, an array of biochemical methods including classic routine- as well as innovative biomarkers has been utilized for the early diagnosis of PEGF. Of these, serum Cystatin C (SCys) has received the most attention. This study was undertaken to evaluate the diagnostic performance of SCys for the segregation between kidney recipients (KRs) with PEGF and GEGF (Good early graft function) using a Latex Enhanced Immunoturbidimetric (LETI) assay.
Materials & Methods: In this prospective study, kidney recipients (n=39) were enrolled. Blood samples were collected at 15 -time points post kidney transplantation (post-KT). Demographic and clinical variables were collected. KRs segregated into: a) PEGF defined as dialysis requirement within the first week after operation and/or SCr≥1.70 mg/dl on 5th post -KT and b) GEGF defined as a SCr<1.70 mg/dl on 5th day Post-KT. SCys was measured on BT-1500 auto-analyzer using LEIT kit. Mann-Whitney U and independent T-test were used for evaluation of any difference between two studied groups. Fisher’s exact test was employed for the analysis of categorical variables. Evaluation of the diagnostic and predictive values were performed using receiver operating characteristic (ROC) analysis.
Results: The mean age of the KRs was 41.6±13.2 years. Distribution of male and female participants were 21 (53.8%) and 18 (46.1%). According to clinical criteria, 27 participants (69.2%) were classified as GEGF, whilst remaining 12 individuals (30.8%) were presented PEGF. Divergence between PEGF and GEGF was detected at 56 and 92 hrs post-surgery (p<0.05). Area under curve (AUC), cut-off value, sensitivity, specificity, positive predictive value and negative predictive value for SCys 56 hrs post-KT were 0.75, 2.72 mg/l, 66.7%, 74.1%, 53.3% and 83.3%a. The respective values for 92 hrs post-KT were 0.7, 2.64 mg/l, 75%, 70%, 52.9% and 86.4%, respectively.
Conclusion: This study has revealed that differentiation between PEGF and GEGF with relatively high sensitivity and specificity was achieved at 54 and 92 hrs post-KT. Further investigations are needed to evaluate the relevance of these finding in clinical terms.
Urmia University of Medical Sciences
Studies in Medical Sciences
2717-008X
29
8
2018
11
1
Frequency of Gastric Lesions in Endoscopic Samples in Urmia Imam Khomeini Hospital
557
563
FA
Fariba
Abbasi
Urmia University of Medical Sciences,
Farzaneh
Sefidgar
Urmia University of Medical Sciences,
Background & Aims: Gastric diseases are common clinical conditions mostly diagnosed by endoscopy. Due to the high prevalence of stomach diseases in Iran and the world, this study was performed to evaluate the frequency of gastric diseases in Urmia Imam Khomeini Hospital.
Materials & Methods: In this analytical-descriptive retrospective study all 2754 gastric pathology reports of endoscopy, derived from samples referring to Urmia Imam Khomeini Hospital during March 2016-March 2017, were studied and histopathologic type of gastric lesions were categorized into four major groups (neoplastic, non-neoplastic, polyps and dysplasia) according to age and gender.
Results: The mean patients’ age was 52.65±17.85 years. The frequency of gastric lesions was as follows: non-neoplastic (89.3%), neoplastic (6.9%), polyps (2.4%) and dysplasia (1.4%). All of them were more frequent above the age of 50. The neoplastic lesions were more frequent in men and non-neoplastic lesions in women (p<0.001). The most frequent non-neoplastic lesion was chronic gastritis (87.4%), mostly without accompanying H.pylori (53.2%). The frequency of gastric ulcer was 6.1%, mostly (79.3%) without accompanying H. pylori. Of neoplastic lesions, 99.5% were malignant (frequently adenocarcinoma) and only one case of benign neoplasm (leiomyoma) was reported.
Conclusion: Regarding the known role of H. pylori in occurrence of gastric ulcers and cancer and the conflicting results obtained in this study, we suggest to use other diagnostic tools such as respiratory test in addition to histopathology in evaluation of H.pylori. Also, the high frequency of gastric lesions over the age of fifty indicates the importance of screening for early diagnosis of neoplasms, especially adenocarcinoma in this age group.
Urmia University of Medical Sciences
Studies in Medical Sciences
2717-008X
29
8
2018
11
1
Primary Squamous Cell Carcinoma with Staghorn Stone: A Case Report
564
568
FA
khadijeh
abdal
Ilam University of Medical Sciences
mohammadreza
hafezi ahmadi
Ilam University of Medical Sciences
Primary squamous cell carcinoma of the kidney is very rare. Due to the lack of clinical signs, diagnosis and treatment is often delayed. Because of the nature of invasive squamous cell carcinoma, most patients experience metastasis and recurrence. Prognosis of the disease is poor and survival of patients has been reported less than 5 years. We report a squamous cell carcinoma with a staghorn stone in the left kidney of a 63-year-old woman.
Urmia University of Medical Sciences
Studies in Medical Sciences
2717-008X
29
8
2018
11
1
PREPARATION AND CHARACTERIZATION OF TUMOR CELL LYSATE AND POLY-IC LOADED POLY ( LACTIC-CO-GLYCOLIC ACID) NANOPARTICLES AND EVALUATION OF THEIR ANTI-TUMOR EFFECTS IN MOUSE MODEL OF BREAST CANCER
584
569
FA
sanaz
Sheikhzadeh
Urmia University
Nowruz
Delirezh
Urmia University
Rahim
Hobbenaghi
Urmia University
Background & Aims: Cancer immunotherapy, despite its many benefits, faces major challenges. Nanoparticles are drug delivery systems that may address these challenges. The goal of this study was to produce the tumor cell lysate and Poly-IC loaded nanoparticles with desirable properties and evaluation of their therapeutic effects in mouse model of breast cancer.
Materials & Methods: Nanoparticles were prepared by the double emulsion solvent-evaporation method, using poly (vinyl alcohol) (PVA) as a surfactant. Effects of molecular weight variation and the degree of hydrolyzation of PVA were investigated for the particle size, polydispersity index, encapsulation efficiency and the residual surfactant percentage of nanoparticles. 4T1 Cell line was used to induce the breast tumor in mice. To evaluate the effectiveness of the vaccine, tumor growth rate, proliferation of splenic cells by MTT assay and Delayed Type Hypersensitivity (DTH) reaction were evaluated.
Results: These results showed that the use of polyvinyl alcohol with a molecular weight of 13-23 kDa and 87-89% hydrolysis, produced nanoparticles with desirable properties. Although injection of nanoparticles containing tumor cell lysate with or without poly-IC to mice, caused a significant decrease in tumor growth, increased splenic lymphocyte proliferation, and delayed type hypersensitivity response in these two groups. But these changes, in the group that received nanoparticles containing the tumor cell lysate with Poly-IC were more significant than other groups.
Conclusion: The results showed that the co-encapsulation of tumor cell lysate and poly-IC in one nanoparticle, due to the effects of Poly-IC on the maturation and activation of dendritic cells, enhance anti-tumor immune responses and can be considered as an effective therapeutic strategy for the treatment of breast cancer.
Urmia University of Medical Sciences
Studies in Medical Sciences
2717-008X
29
8
2018
11
1
STUDY THE EFFECT OF FINASTERIDE MICROEMULSION GEL ON THE TREATMENT OF MEN WITH MILD TO MODERATE ACNE
585
592
FA
Efat
Khodayani
Tabriz University of Medical Science, Tabriz, Iran
Elham
Nah chami
Tabriz University of Medical Science, Tabriz, Iran
Mahdi
Amirnia
Tabriz University of Medical Science, Tabriz, Iran
Javad
Shokri
Tabriz University of Medical Science, Tabriz, Iran
Amirreza
Jahanshahi
Tabriz University of Medical Science, Tabriz, Iran
Azin
Nah chami
, Islamic Azad of Tehran University of Medical Science, Tehran, Iran
Mohammad Javad
Naghizadeh
Ardabil University of Medical Science
Background & Aims: Acne vulgaris is a common disorder of adolescence which may lead to significant cosmetic and psychological problems. About 70% of adolescents suffer from acne. One of the most common abnormalities that patients seek for skin care. Acne is a chronic and inflammatory disease that affects the sebaceous glands of the follicles and has a significant outbreak, especially among adolescents and young people. The objective of this randomized, double-blind clinical trial is evaluation of the effect of Finasteride microemulsion gel in the treatment of men with mild to moderate acne.
Materials & Methods: In this clinical trial, 48 men with mild to moderate acne assigned into the study randomly and the subjects divided into two groups A and B randomly. Patients evaluated by a physician in terms of clinical manifestations of acne (number of lesions, acne severity, patient satisfaction). The two groups will be followed up for 3 months. Finasteride microemulsion gel twice in a day and 1% Clindamycin phosphate topical gel twice in a day will be administered for three months for group (A) and 1% Clindamycin phosphate topical gel twice in a day will be administered for three months for group (B). If any complications are detected, the patient will be excluded from the study. Then two weeks, one month, and three months after the intervention will be followed up respectively.
Results: The mean age of the patients in the case group was 22.24 ± 0.7 years with a range of 15-28 years old and in the control group 21.7 ± 0.63 years with a range of age from 30 to 16 years. The mean of total papules at the onset of treatment was 3.5 ± 3.5 in the case group and 4.1 ± 4 in the control group and there was no significant difference between the two groups. At the end of the third month, the treatment group had a mean of 0.9 ± 0.3 in the case group and 1.1 ± 0.6 in the control group and the mean difference between the two groups was statistically significant.
Conclusion: According to the results of this study, Finasteride microemulsion gel had a good effect on the therapeutic results and it is recommended to doctors and physicians.
Urmia University of Medical Sciences
Studies in Medical Sciences
2717-008X
29
8
2018
11
1
ASSESSMENT EFFECTIVE FACTORS OF QUALITY PLANAR NUCLEAR MEDICINE IMAGES USING MODULATION TRANSFER FUNCTION
593
602
FA
Hassan
Moladoust
Medical Physicist (PhD), Healthy Heart Research Center, Guilan University of Medical Sciences, Rasht, Iran
Ahmad
Bitarafan Rajabi
Medical Physicist (PhD), Department of Nuclear Medicine and Cardiovascular Interventional Research Center, Rajaie Cardiovascular Medical & Research Center, Iran University of Medical Sciences, Tehran, Iran (Corresponding Author)
Vahid
Nikseresht
Cardiologist (MD), Healthy Heart Research Center, Guilan University of Medical Sciences, Rasht, Iran
Background & Aims: Spatial resolution is one of the important features of evaluating the function of nuclear medicine imaging systems. The aim of this study was evaluating the capability of the spatial resolution in images of nuclear medicine planar by calculating the modulation transfer function (MTF).
Materials & Methods: Nuclear medicine imaging of capillary tube with activity 1 mCi was performed by planar method in different distances and different matrix sizes, in two different environments as follows: without scattered radiation (under ideal condition); with scattered radiation (in real condition by filling water phantom). Then the line spread function for each of them was obtained by drawing a linear profile on the images and MTF and the error of sum of squares were calculated by using Gaussian curve fitting and Fourier transform.
Results: The results of calculating MTF in the planar images showed that under ideal condition, as well as in real condition, MTF is a function of the distance that exists between the source and the detector and the matrix size. The error sum of squares in different imaging conditions, changed and it increased by increasing the distance and matrix size. (0.84 to 61.29 in real conditions and from 0.10 to 57.02 in ideal conditions).
Conclusion: The presence of scattered radiation had a significant effect on the spatial resolution of imaging system. We can achieve more suitable spatial resolution by using larger matrix size and also reducing the distance of source and the detector. Having accurate awareness about spatial resolution in the imaging systems in different matrix sizes and different distances can be useful in nuclear medicine imaging.
Urmia University of Medical Sciences
Studies in Medical Sciences
2717-008X
29
8
2018
11
1
ASSOCIATION OF TCF7L2 rs11196205(C/G) and CAPN10 rs3792267(G/A) polymorphisms with type II diabetes in Arab population of Khuzestan province
603
613
FA
abdollah
gravand
Shahid Chamran University
Ali Mohammad
foroughmand
Shahid Chamran University
Mehdi
Pourmehdi Boroujeni
Shahid Chamran University
Background & Aims: Type II diabetes is a multifactorial and heterogenic disease that is characterized by a defect in the production or function of insulin. In recent years, through Genome-wide association studies (GWAS) has been shown the association of several genes with Type II diabetes. The aim of this study was to investigate the relationship of polymorphisms TCF7L2 rs11196205 and CAPN10 rs3792267 with type II diabetes in the Arab population of Khuzestan province.
Materials & Methods: A case-control study was performed using 98 T2DM patients and 96 Controls. Genotyping for rs11196205 was done by TETRA-Primer ARMS-PCR and for rs3792267 was done by PCR-RFLP Technique.
Results: In examining rs11196205 based on the genotype GG, results for CG genotype were, OR = 1.29, 95%CI = (0.58–2.86), P -value = 0.54 and for genotype CC were, OR = 2.62, 95%CI = (1.06–6.44), P -value = 0.037. Also, the frequencies of C and G alleles in p atients with diabetes were 60.71% and 39.28%, resp ectively, and in the healthy subjects was 50% for each of the alleles (OR = 1.55, 95%CI = (1.03–2.31), P -value = 0.04). There was no significant association with type II diabetes for rs3792267 in the studied population. Conclusion: Our results for TCF7L2 rs11196205 polymorphism showed that CC genotype and C allele increase the risk of developing type II diabetes in the Arab population of Khuzestan province.
Urmia University of Medical Sciences
Studies in Medical Sciences
2717-008X
29
8
2018
11
1
Evaluation of the prevalence of oral pigmentation in patients referred to Urmia dental faculty in 2017
614
620
FA
zahra
mirzaei
School of Dentistry, Urmia University of Medical Sciences, Urmia, Iran
ehsan
khashabi
School of Dentistry, Urmia University of Medical Sciences, Urmia, Iran
Negar
Sarrafan
School of Dentistry, Urmia University of Medical Sciences, Urmia, Iran
Background & Aims: Oral mucosa has a pink color in healthy people and can vary from light to dark. Gingiva may get discolored due to internal and external causes. One of the causative factors is melanin pigmentation. Melanin is brownish pigment that is not derived from hemoglobin, and accounts for some of the natural discoloration and pigmentation of gingiva and other mucosal layers of oral cavity.
Materials & Methods: This descriptive cross-sectional study was carried out in Oral Diseases Ward of Urmia Dentistry School since May 2016 to May 2017. In this study, 476 patients underwent procedures and were evaluated in a randomized manner.
Results: In this study, 476 patients were studied and among them, 133 ones (27.94%) had mucosal pigmentation. Considering the severity of pigmentation; 73 patients (54.9%) had mild pigmentation, 22 (16.5%) had moderate pigmentation, and 38 (28.6%) had severe pigmentation. There was a significant correlation between oral pigmentation and gender (p=0.036) and the age of patients (p=0.001).
Conclusion: Mucosal pigmentation in Azerbaijan and exclusively Urmia city has concordance with the patterns across the country and has a significant association with patients’ gender and age. In this way pigmentation is seen more frequently in male patients with lower age.