Studies in Medical Sciences
مجله مطالعات علوم پزشکی
Studies in Medical Sciences
Medical Sciences
http://umj.umsu.ac.ir
37
journal37
2717-008X
2717-008X
10.61186/umj
fa
jalali
1397
7
1
gregorian
2018
10
1
29
7
online
1
fulltext
en
فاقدکاراکترفارسی است
Role of PA3574 (nalD) gene in development of ciprofloxacin resistance in Pseudomonas aeruginosa isolates
میکروبیولوژی
میکروبیولوژی
پژوهشي(توصیفی- تحلیلی)
Research
فاقدکاراکترفارسی است
<strong><em>Background &</em></strong> <strong><em>Aims</em></strong>: Pseudomonas aeruginosa is a gram-negative opportunistic pathogen and one of the mortality causes of nosocomial infections. One of the drug resistance mechanisms in P.aeruginosa is mutation in negative regulator genes of mexAB-oprM efflux pump system such as nalD. The aim of this study was to investigate the role of nalD mutations in P. aeruginosa isolates of Guilan province in ciprofloxacin resistant development.<br>
<strong><em>Materials & Methods</em></strong><strong>:</strong> In this study, 45 P. aeruginosa isolates were obtained from different clinical samples of Rasht and Lahijan hospitals and laboratories between 2014 to 2016 and identified by biochemical tests. Ciprofloxacin resistance and susceptibility of the strains was determined by disc difusion and MIC methods. PCR-sequencing was performed to assess nalD gene mutations in ciprofloxacin resistant strains.<br>
<strong><em>Results</em></strong><strong>:</strong> Seventeen from 45 isolates of P.aeruginosa were ciprofloxacin resistant (MIC≥1024 µg/ml). PCR-sequencing analysis showed that one resistant isolate had one- C nucleotide deletion in codon 193 (p.Leu193CysfsX, in gene level<span dir="RTL">:</span> c.577delC)) (c.577delC) and three resistant isolates had L153Q missense mutations in nalD gene.<br>
<strong><em>Conclusion</em></strong><strong>:</strong> It appears that mutations of nalD gene lead to overexpression of mexAB-oprM and subsequently ciprofloxacin resistance in P.aeruginosa isolates in Guilan province.
Ciprofloxacin, mutation, nalD, Pseudomonas aeruginosa, Sequencing.
Ciprofloxacin, mutation , nalD, Pseudomonas aeruginosa, Sequencing.
502
510
http://umj.umsu.ac.ir/browse.php?a_code=A-10-2705-2&slc_lang=en&sid=1
Saeid
Rahbar Takrami
Saeid
Rahbar Takrami
rahbar_saeid@yahoo.com
3700319475328460022150
3700319475328460022150
No
rasht branch , islamic azad university
فاقدکاراکترفارسی است
najmeh
ranji
najmeh
ranji
najmehranji@gmail.com
3700319475328460022151
3700319475328460022151
Yes
rasht branch , islamic azad university
فاقدکاراکترفارسی است